SESN1 (NM_014454) Human Tagged ORF Clone

CAT#: RC211305

SESN1 (Myc-DDK-tagged)-Human sestrin 1 (SESN1), transcript variant 1

Product Images

Circular map for RC211305

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SESN1
• Synonyms: PA26; SEST1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211305 representing NM_014454
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGAAGGAGAGAATGAAGTGAGATGGGATGGACTCTGCAGCAGAGATTCAACTACTAGGGAGACAG
  CATTGGAAAACATTAGGCAAACCATTTTGAGGAAAACCGAGTATCTTCGTTCGGTGAAAGAAACACCTCA
  TCGTCCATCAGACGGGCTTTCAAATACCGAGTCTTCGGATGGGTTGAATAAGCTACTTGCTCATCTGCTT
  ATGCTTTCTAAGAGGTGTCCCTTCAAAGATGTGAGAGAGAAAAGTGAGTTTATTCTGAAGAGCATCCAGG
  AACTTGGCATTAGAATTCCTCGACCACTAGGACAGGGACCAAGCAGATTCATCCCAGAAAAGGAGATCCT
  CCAAGTGGGGAGTGAAGACGCACAGATGCATGCTTTATTTGCAGATTCTTTTGCTGCTTTGGGCCGTTTG
  GATAACATTACGTTAGTGATGGTTTTCCACCCACAATATTTAGAAAGTTTCTTAAAAACTCAGCACTATC
  TACTGCAAATGGATGGGCCGTTACCCCTACATTATCGTCACTACATTGGAATAATGGCTGCGGCAAGACA
  TCAGTGCTCCTACTTAGTGAACCTGCATGTAAATGATTTCCTTCATGTTGGTGGGGACCCCAAGTGGCTC
  AATGGTTTAGAGAATGCTCCTCAAAAACTACAGAATTTAGGAGAACTTAACAAAGTGTTAGCCCATAGAC
  CTTGGCTTATTACCAAAGAACACATTGAGGGACTTTTAAAAGCTGAAGAGCACAGCTGGTCCCTTGCGGA
  ATTGGTACATGCAGTAGTTTTACTCACACACTATCATTCTCTTGCCTCATTCACATTCGGCTGTGGAATC
  AGTCCAGAAATTCATTGTGATGGTGGCCACACATTCAGACCTCCTTCTGTTAGCAACTACTGCATCTGTG
  ACATTACAAATGGCAATCACAGTGTGGATGAGATGCCGGTCAACTCAGCAGAAAATGTTTCTGTAAGTGA
  TTCTTTCTTTGAGGTTGAAGCCCTCATGGAAAAGATGAGGCAGTTACAGGAATGTCGAGATGAAGAAGAG
  GCAAGTCAGGAAGAGATGGCTTCACGTTTTGAAATAGAAAAAAGAGAGAGTATGTTTGTCTTCTCTTCAG
  ATGATGAAGAAGTTACACCAGCAAGAGCTGTATCTCGTCATTTTGAGGATACTAGTTATGGCTATAAAGA
  TTTCTCTAGACATGGGATGCATGTTCCAACATTTCGTGTCCAGGACTATTGCTGGGAAGATCATGGTTAT
  TCTTTGGTAAATCGCCTTTATCCAGATGTGGGACAGTTGATTGATGAAAAATTTCACATTGCTTACAATC
  TTACTTATAATACAATGGCAATGCACAAAGATGTTGATACCTCAATGCTTAGACGGGCAATTTGGAACTA
  TATTCACTGCATGTTTGGAATAAGATATGATGATTATGACTATGGTGAAATTAACCAGCTATTGGATCGT
  AGCTTTAAAGTTTATATCAAAACTGTTGTTTGCACTCCTGAAAAGGTTACCAAAAGAATGTATGATAGCT
  TCTGGAGGCAGTTCAAGCACTCTGAGAAGGTTCATGTTAATCTGCTTCTTATAGAAGCTAGGATGCAAGC
  AGAACTCCTTTATGCTCTGAGAGCCATTACCCGCTATATGACC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211305 representing NM_014454
  Red=Cloning site Green=Tags(s)
  
  MAEGENEVRWDGLCSRDSTTRETALENIRQTILRKTEYLRSVKETPHRPSDGLSNTESSDGLNKLLAHLL
  MLSKRCPFKDVREKSEFILKSIQELGIRIPRPLGQGPSRFIPEKEILQVGSEDAQMHALFADSFAALGRL
  DNITLVMVFHPQYLESFLKTQHYLLQMDGPLPLHYRHYIGIMAAARHQCSYLVNLHVNDFLHVGGDPKWL
  NGLENAPQKLQNLGELNKVLAHRPWLITKEHIEGLLKAEEHSWSLAELVHAVVLLTHYHSLASFTFGCGI
  SPEIHCDGGHTFRPPSVSNYCICDITNGNHSVDEMPVNSAENVSVSDSFFEVEALMEKMRQLQECRDEEE
  ASQEEMASRFEIEKRESMFVFSSDDEEVTPARAVSRHFEDTSYGYKDFSRHGMHVPTFRVQDYCWEDHGY
  SLVNRLYPDVGQLIDEKFHIAYNLTYNTMAMHKDVDTSMLRRAIWNYIHCMFGIRYDDYDYGEINQLLDR
  SFKVYIKTVVCTPEKVTKRMYDSFWRQFKHSEKVHVNLLLIEARMQAELLYALRAITRYMT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014454
• ORF Size: 1653 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014454.3
• RefSeq Size: 3212 bp
• RefSeq ORF: 1656 bp
• Locus ID: 27244
• UniProt ID: Q9Y6P5
• Cytogenetics: 6q21
• Domains: PA26
• Protein Pathways: p53 signaling pathway
• MW: 64.3 kDa
• Gene Summary: This gene encodes a member of the sestrin family. Sestrins are induced by the p53 tumor suppressor protein and play a role in the cellular response to DNA damage and oxidative stress. The encoded protein mediates p53 inhibition of cell growth by activating AMP-activated protein kinase, which results in the inhibition of the mammalian target of rapamycin protein. The encoded protein also plays a critical role in antioxidant defense by regenerating overoxidized peroxiredoxins, and the expression of this gene is a potential marker for exposure to radiation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2010]