BAPX1 (NKX3-2) (NM_001189) Human Tagged ORF Clone

CAT#: RC211285

NKX3 (Myc-DDK-tagged)-Human NK3 homeobox 2 (NKX3-2)

ORF Plasmid: DDK tGFP

Lentiviral Particles: DDK DDK w/ Puro mGFP mGFP w/ Puro


  "NM_001189" in other vectors (6)

Reconstitution Protocol

USD 450.00

In Stock*

Size
    • 10 ug

Product Images

Frequently bought together (3)
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DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol BAPX1
Synonyms BAPX1; NKX3.2; NKX3B; SMMD
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC211285 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGTGCGCGGCGCCAACACCTTGACGTCCTTCTCCATCCAGGCGATCCTCAACAAGAAAGAGGAGC
GCGGCGGGCTGGCCGCGCCAGAGGGGCGCCCGGCGCCCGGGGGCACAGCGGCATCGGTGGCCGCGGCTCC
CGCTGTCTGCTGTTGGCGGCTCTTTGGGGAGAGGGACGCGGGCGCGTTGGGGGGCGCCGAGGACTCTCTG
CTGGCGTCTCCTGCCGGTACCAGAACAGCTGCGGGGCGGACTGCGGAGAGCCCGGAAGGCTGGGACTCGG
ACTCCGCGCTCAGCGAGGAGAACGAGAGCAGGCGGCGCTGCGCGGACGCGCGGGGGGCCAGCGGGGCCGG
CCTTGCGGGGGGATCCTTGAGCCTCGGCCAGCCGGTCTGTGAGCTGGCCGCTTCCAAAGACCTAGAGGAG
GAAGCCGCGGGCCGGAGCGACAGCGAGATGTCCGCCAGCGTCTCAGGCGACCGCAGCCCAAGGACCGAGG
ACGACGGTGTTGGCCCCAGAGGTGCACACGTGTCCGCGCTGTGCAGCGGGGCCGGCGGCGGGGGCGGCAG
CGGGCCGGCAGGCGTCGCGGAGGAGGAGGAGGAGCCGGCGGCGCCCAAGCCACGCAAGAAGCGCTCGCGG
GCCGCTTTCTCCCACGCGCAGGTCTTCGAGCTGGAGCGCCGCTTTAACCACCAGCGCTACCTGTCCGGGC
CCGAGCGCGCAGACCTGGCCGCGTCGCTGAAGCTCACCGAGACGCAGGTGAAAATCTGGTTCCAGAACCG
TCGCTACAAGACAAAGCGCCGGCAGATGGCAGCCGACCTGCTGGCCTCGGCGCCCGCCGCCAAGAAGGTG
GCCGTAAAGGTGCTGGTGCGCGACGACCAGAGACAATACCTGCCCGGCGAAGTGCTGCGGCCACCCTCGC
TTCTGCCACTGCAGCCCTCCTACTATTACCCGTACTACTGCCTCCCAGGCTGGGCGCTCTCCACCTGCGC
AGCTGCCGCAGGCACCCAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC211285 protein sequence
Red=Cloning site Green=Tags(s)

MAVRGANTLTSFSIQAILNKKEERGGLAAPEGRPAPGGTAASVAAAPAVCCWRLFGERDAGALGGAEDSL
LASPAGTRTAAGRTAESPEGWDSDSALSEENESRRRCADARGASGAGLAGGSLSLGQPVCELAASKDLEE
EAAGRSDSEMSASVSGDRSPRTEDDGVGPRGAHVSALCSGAGGGGGSGPAGVAEEEEEPAAPKPRKKRSR
AAFSHAQVFELERRFNHQRYLSGPERADLAASLKLTETQVKIWFQNRRYKTKRRQMAADLLASAPAAKKV
AVKVLVRDDQRQYLPGEVLRPPSLLPLQPSYYYPYYCLPGWALSTCAAAAGTQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001189
ORF Size 999 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001189.4
RefSeq Size 2241 bp
RefSeq ORF 1002 bp
Locus ID 579
UniProt ID P78367
Cytogenetics 4p15.33
MW 34.8 kDa
Gene Summary This gene encodes a member of the NK family of homeobox-containing proteins. The encoded protein may play a role in skeletal development. [provided by RefSeq, Jul 2008]

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