RNF152 (NM_173557) Human Tagged ORF Clone

CAT#: RC211280

RNF152 (Myc-DDK-tagged)-Human ring finger protein 152 (RNF152)

Product Images

Circular map for RC211280

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  Western blot validation of overexpression lysate (Cat# LY406468) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC211280 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RNF152
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211280 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGACGCTGTCCCAGGACTCTCTGCTGGAATGTCAGATCTGTTTCAATTACTACAGCCCCCGGCGCA
  GGCCCAAGTTGCTGGACTGCAAGCACACCTGCTGTTCAGTGTGCCTGCAGCAGATGAGGACCAGCCAGAA
  GGATGTGCGGTGCCCCTGGTGCCGCGGTGTCACCAAGCTGCCTCCCGGCTTCTCCGTGTCGCAGCTCCCG
  GACGACCCGGAGGTCCTGGCTGTCATCGCCATTCCACACACTTCCGAACACACCCCGGTCTTCATCAAAC
  TTCCCAGCAATGGGTGCTACATGCTGCCCCTGCCCATCTCCAAGGAGCGTGCGCTGCTGCCCGGAGACAT
  GGGCTGCCGCCTGCTGCCCGGGAGCCAGCAGAAGTCCGTCACCGTGGTGACCATCCCTGCTGAACAGCAG
  CCTCTGCAAGGTGGGGCTCCCCAGGAGGCGGTGGAGGAGGAGCAGGACAGGCGGGGCGTGGTGAAAAGCT
  CCACCTGGTCGGGGGTGTGCACTGTCATCTTGGTGGCTTGCGTCTTGGTCTTCCTCCTCGGCATCGTGCT
  TCACAACATGTCTTGCATTTCTAAGCGCTTCACTGTGATATCCTGTGGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211280 protein sequence
  Red=Cloning site Green=Tags(s)
  
  METLSQDSLLECQICFNYYSPRRRPKLLDCKHTCCSVCLQQMRTSQKDVRCPWCRGVTKLPPGFSVSQLP
  DDPEVLAVIAIPHTSEHTPVFIKLPSNGCYMLPLPISKERALLPGDMGCRLLPGSQQKSVTVVTIPAEQQ
  PLQGGAPQEAVEEEQDRRGVVKSSTWSGVCTVILVACVLVFLLGIVLHNMSCISKRFTVISCG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_173557
• ORF Size: 609 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_173557.3
• RefSeq Size: 1805 bp
• RefSeq ORF: 612 bp
• Locus ID: 220441
• UniProt ID: Q8N8N0
• Cytogenetics: 18q21.33
• Protein Families: Transmembrane
• MW: 22.4 kDa
• Gene Summary: E3 ubiquitin-protein ligase mediating 'Lys-63'-linked polyubiquitination of RRAGA in response to amino acid starvation. Thereby, regulates mTORC1 signaling and plays a role in the cellular response to amino acid availability (PubMed:25936802). Also mediates 'Lys-48'-linked polyubiquitination of target proteins and their subsequent targeting to the proteasome for degradation. Induces apoptosis when overexpressed (PubMed:21203937).[UniProtKB/Swiss-Prot Function]