ABCD2 (NM_005164) Human Tagged ORF Clone

CAT#: RC211199

ABCD2 (Myc-DDK-tagged)-Human ATP-binding cassette, sub-family D (ALD), member 2 (ABCD2)

Product Images

Circular map for RC211199

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY ABCD2 (Cat# RC211199, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ABCD2 (Cat# TA810066)(1:500). Positive lysates LY417474 (100ug) and LC417474 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY417474) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC211199 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ABCD2
• Synonyms: ABC39; ALDL1; ALDR; ALDRP; hALDR
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211199 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACACATATGCTAAATGCAGCAGCTGATCGAGTGAAATGGACCAGATCGAGTGCTGCTAAGAGGGCTG
  CCTGCCTGGTGGCTGCGGCATATGCTCTGAAAACCCTCTATCCCATCATTGGCAAGCGTTTAAAGCAATC
  TGGCCACGGGAAGAAAAAAGCAGCAGCTTACCCTGCTGCAGAGAACACAGAAATACTGCATTGCACCGAG
  ACCATTTGTGAAAAACCTTCGCCTGGAGTGAATGCAGATTTCTTCAAACAGCTACTAGAACTTCGGAAAA
  TTTTGTTTCCAAAACTTGTGACCACTGAAACAGGGTGGCTCTGCCTGCACTCAGTGGCTCTAATCTCAAG
  AACCTTTCTTTCTATCTATGTGGCTGGTCTGGATGGAAAAATCGTGAAAAGCATTGTGGAAAAGAAGCCT
  CGGACTTTCATCATCAAATTAATCAAGTGGCTTATGATTGCCATCCCTGCTACCTTCGTCAACAGTGCAA
  TAAGGTACCTGGAATGCAAATTGGCTTTGGCCTTCAGAACTCGCCTAGTAGACCACGCCTATGAAACCTA
  TTTTACAAATCAGACTTATTATAAAGTGATCAATATGGATGGGAGGCTGGCAAACCCTGACCAATCTCTT
  ACGGAGGATATTATGATGTTCTCCCAATCTGTGGCTCACTTGTATTCCAATCTGACCAAACCTATTTTAG
  ATGTAATGCTGACCTCCTATACACTCATTCAAACTGCTACATCCAGAGGAGCAAGCCCAATTGGGCCCAC
  CCTACTAGCAGGACTTGTGGTGTATGCCACTGCTAAAGTGTTAAAAGCCTGTTCTCCCAAATTTGGCAAA
  CTGGTGGCAGAGGAAGCACATAGAAAAGGCTATTTGCGGTATGTGCACTCGAGAATTATAGCCAATGTAG
  AAGAAATTGCCTTTTACAGAGGACATAAGGTAGAAATGAAACAACTTCAGAAAAGTTACAAAGCTTTAGC
  AGATCAGATGAACCTCATTTTATCCAAACGTTTGTGGTACATCATGATAGAACAGTTCCTGATGAAGTAT
  GTTTGGAGCAGCAGTGGACTAATTATGGTGGCTATACCTATTATCACTGCAACTGGCTTTGCAGATGGTG
  AGGATGGCCAAAAGCAAGTTATGGTTAGTGAACGGACAGAAGCCTTTACCACTGCTCGAAATTTACTGGC
  CTCTGGAGCTGATGCTATTGAAAGGATTATGTCTTCATACAAAGAGGTCACTGAATTAGCAGGCTACACT
  GCTCGAGTGTACAATATGTTTTGGGTCTTTGATGAAGTAAAAAGAGGCATTTATAAGAGAACTGCTGTCA
  TTCAAGAATCTGAAAGCCATAGCAAGAATGGAGCTAAGGTAGAATTACCTCTCAGTGACACATTGGCAAT
  TAAAGGAAAAGTTATTGATGTGGATCACGGAATTATTTGTGAAAATGTTCCCATAATTACACCAGCAGGA
  GAAGTGGTGGCTTCCAGGCTAAACTTCAAAGTAGAAGAAGGAATGCATCTTTTGATAACTGGTCCCAATG
  GTTGTGGGAAAAGTTCTCTCTTCAGAATTCTAAGTGGGCTCTGGCCTGTGTATGAAGGAGTCCTCTATAA
  ACCACCTCCTCAACATATGTTTTATATTCCACAAAGGCCATATATGTCTCTTGGAAGTCTTCGGGATCAA
  GTCATTTACCCTGATTCAGTGGATGATATGCATGATAAAGGTTATACAGACCAAGATCTGGAACGTATCC
  TACACAATGTCCATCTCTATCACATAGTTCAAAGAGAAGGAGGATGGGATGCTGTTATGGACTGGAAAGA
  TGTCCTGTCAGGAGGGGAAAAGCAAAGAATGGGCATGGCTCGTATGTTTTATCATAAACCAAAATATGCC
  TTGCTGGATGAATGTACCAGTGCTGTCAGCATTGATGTCGAAGGAAAGATATTTCAGGCTGCAAAAGGGG
  CTGGAATTTCCTTACTGTCTATAACACACAGACCTTCTCTTTGGAAATACCACACACATTTATTACAGTT
  TGATGGTGAAGGAGGTTGGCGCTTTGAACAATTGGATACTGCTATCCGTTTGACATTGAGTGAAGAAAAA
  CAAAAGCTAGAATCTCAGCTAGCTGGAATTCCCAAAATGCAGCAGAGACTCAATGAACTATGTAAAATTT
  TGGGAGAAGACTCAGTGCTGAAAACAATTAAAAATGAAGATGAGACATCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211199 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MTHMLNAAADRVKWTRSSAAKRAACLVAAAYALKTLYPIIGKRLKQSGHGKKKAAAYPAAENTEILHCTE
  TICEKPSPGVNADFFKQLLELRKILFPKLVTTETGWLCLHSVALISRTFLSIYVAGLDGKIVKSIVEKKP
  RTFIIKLIKWLMIAIPATFVNSAIRYLECKLALAFRTRLVDHAYETYFTNQTYYKVINMDGRLANPDQSL
  TEDIMMFSQSVAHLYSNLTKPILDVMLTSYTLIQTATSRGASPIGPTLLAGLVVYATAKVLKACSPKFGK
  LVAEEAHRKGYLRYVHSRIIANVEEIAFYRGHKVEMKQLQKSYKALADQMNLILSKRLWYIMIEQFLMKY
  VWSSSGLIMVAIPIITATGFADGEDGQKQVMVSERTEAFTTARNLLASGADAIERIMSSYKEVTELAGYT
  ARVYNMFWVFDEVKRGIYKRTAVIQESESHSKNGAKVELPLSDTLAIKGKVIDVDHGIICENVPIITPAG
  EVVASRLNFKVEEGMHLLITGPNGCGKSSLFRILSGLWPVYEGVLYKPPPQHMFYIPQRPYMSLGSLRDQ
  VIYPDSVDDMHDKGYTDQDLERILHNVHLYHIVQREGGWDAVMDWKDVLSGGEKQRMGMARMFYHKPKYA
  LLDECTSAVSIDVEGKIFQAAKGAGISLLSITHRPSLWKYHTHLLQFDGEGGWRFEQLDTAIRLTLSEEK
  QKLESQLAGIPKMQQRLNELCKILGEDSVLKTIKNEDETS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005164
• ORF Size: 2220 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005164.4
• RefSeq Size: 5341 bp
• RefSeq ORF: 2223 bp
• Locus ID: 225
• UniProt ID: Q9UBJ2
• Cytogenetics: 12q12
• Domains: ABC_tran, AAA
• Protein Families: Druggable Genome
• Protein Pathways: ABC transporters
• MW: 83.2 kDa
• Gene Summary: The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ALD subfamily, which is involved in peroxisomal import of fatty acids and/or fatty acyl-CoAs in the organelle. All known peroxisomal ABC transporters are half transporters which require a partner half transporter molecule to form a functional homodimeric or heterodimeric transporter. The function of this peroxisomal membrane protein is unknown; however this protein is speculated to function as a dimerization partner of ABCD1 and/or other peroxisomal ABC transporters. Mutations in this gene have been observed in patients with adrenoleukodystrophy, a severe demyelinating disease. This gene has been identified as a candidate for a modifier gene, accounting for the extreme variation among adrenoleukodystrophy phenotypes. This gene is also a candidate for a complement group of Zellweger syndrome, a genetically heterogeneous disorder of peroxisomal biogenesis. [provided by RefSeq, Jul 2008]