KIF19 (NM_153209) Human Tagged ORF Clone

CAT#: RC211178

KIF19 (Myc-DDK-tagged)-Human kinesin family member 19 (KIF19)

Product Images

Circular map for RC211178

• 

  Western blot validation of overexpression lysate (Cat# LY407138) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC211178 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: KIF19
• Synonyms: KIF19A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211178 representing NM_153209
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGGACAGCGGGGACTCCAAGGACCAGCAACTCATGGTGGCGCTTCGGGTCCGGCCCATCAGCGTGG
  CAGAGCTGGAGGAAGGAGCTACCCTCATCGCCCATAAAGTGGATGAGCAGATGGTGGTTCTCATGGACCC
  AATGGAGGATCCCGACGACATCCTGCGGGCGCATCGCTCCCGGGAGAAGTCCTACCTGTTCGACGTGGCC
  TTTGACTTCACCGCCACCCAGGAGATGGTGTATCAGGCCACCACCAAGAGCCTCATCGAGGGCGTCATCT
  CAGGCTACAATGCCACTGTCTTTGCCTATGGCCCCACAGGCTGTGGGAAAACCTACACCATGCTGGGCAC
  AGACCAGGAGCCTGGCATCTATGTTCAGACCCTCAACGACCTCTTCCGTGCCATCGAGGAGACCAGCAAT
  GACATGGAGTATGAGGTCTCCATGTCCTACCTGGAGATCTACAATGAGATGATCCGGGACCTGCTGAACC
  CCTCCCTGGGCTACCTGGAGCTGCGGGAGGACTCTAAGGGGGTGATCCAGGTGGCCGGCATCACCGAAGT
  CTCCACCATCAATGCCAAGGAGATCATGCAGCTGCTGATGAAGGGGAACCGGCAGAGGACCCAGGAGCCC
  ACGGCCGCCAACCAGACGTCCTCCCGCTCCCACGCGGTACTGCAGGTGACCGTGCGCCAGCGCAGCCGGG
  TCAAGAACATCTTGCAGGAGGTGCGGCAGGGCCGCCTGTTCATGATCGACCTGGCTGGCTCAGAGCGCGC
  CTCGCAGACACAGAATCGTGGGCAGCGTATGAAGGAGGGGGCCCACATCAACCGCTCACTGCTGGCACTG
  GGCAACTGCATCAACGCCCTGAGCGACAAGGGTAGCAACAAGTACATCAACTATCGCGACAGCAAGCTCA
  CCCGGCTCCTGAAGGACTCTCTGGGAGGAAACAGCCGCACAGTGATGATCGCTCACATCAGTCCTGCGAG
  CAGTGCCTTCGAGGAGTCCCGGAACACCCTGACCTACGCCGGCCGGGCCAAGAACATTAAGACTAGGGTG
  AAGCAGAACCTCCTGAACGTCTCCTACCACATCGCCCAGTACACCAGCATCATCGCTGACCTGCGGGGCG
  AGATCCAGCGACTCAAGCGCAAGATTGATGAGCAGACTGGGCGGGGCCAGGCCCGGGGCCGGCAGGATCG
  GGGTGACATCCGCCACATCCAAGCTGAGGTCCAGCTGCACAGCGGGCAGGGTGAGAAGGCTGGCATGGGA
  CAGCTTCGGGAGCAGCTCGCCAGCGCCTTCCAGGAGCAGATGGATGTGCGGAGGCGCCTGCTGGAGCTGG
  AGAACCGCGCCATGGAGGTCCAGATTGACACCTCCCGACACCTGCTCACCATCGCCGGCTGGAAGCATGA
  GAAGTCCCGCCGGGCCCTCAAATGGCGGGAGGAGCAGCGAAAGGAGTGCTACGCTAAGGACGACAGCGAG
  AAGGACTCAGACACAGGTGATGACCAACCAGACATCCTGGAGCCACCCGAGGTGGCCGCAGCCCGGGAGA
  GCATTGCAGCCCTGGTGGACGAGCAGAAGCAACTGCGCAAGCAGAAGCTGGCGCTGGAGCAGCGCTGCCG
  GGAGCTGCGCGCGCGGGGCCGGCGCCTGGAGGAGACGCTGCCGCGGCGCATCGGCTCCGAGGAGCAGCGC
  GAGGTGCTCAGCCTGCTGTGCCGCGTGCACGAGCTCGAGGTGGAGAACACCGAGATGCAGTCGCACGCGC
  TGCTCCGCGACGGTGCGCTCCGCCACCGCCACGAGGCCGTGCGCCGCCTGGAGCAGCACCGCAGTCTCTG
  CGACGAGATTATCCAGGGCCAGCGGCAGATCATCGACGACTACAACCTGGCCGTCCCGCAGCGCCTGGAA
  GAGCTCTACGAAGTGTACCTGCGGGAGCTGGAGGAGGGCAGCCTGGAGCAGGCCACCATCATGGACCAAG
  TGGCCTCCAGGGCCCTGCAGGACAGCTCCTTGCCCAAAATTACCCCAGCAGGAACCTCACTGACCCCAGA
  TTCTGACCTGGAGAGTGTGAAGACATTGAGCTCTGATGCCCAGCACCTGCAGAACAGCGCCCTCCCTCCC
  CTCAGCACAGAGAGTGAAGGCCACCACGTGTTCAAGGCTGGTACTGGGGCCTGGCAGGCAAAAAGCTCCT
  CTGTGCCCACCCCACCTCCCATCCAGCTCGGCAGCCTGGTGACGCAGGAGGCCCCGGCTCAGGACAGCCT
  GGGCAGCTGGATCAACTCTTCCCCTGACAGCAGTGAGAACCTGTCGGAGATCCCCTTGTCCCACAAAGAG
  AGGAAGGAGATCCTGACTGGCACCAAGTGCATCTGGGTGAAGGCCGCCCGGCGGCGCTCGCGGGCCCTGG
  GAACCGAGGGGCGACACCTGCTGGCACCCGCGACAGAGCGCAGCAGCCTGTCCCTGCACTCACTGAGCGA
  GGGCGACGATGCGCGGCCACCAGGCCCACTGGCCTGCAAGCGGCCGCCCAGCCCCACACTACAGCATGCT
  GCCAGTGAGGACAACCTGTCCAGCAGCACGGGCGAGGCCCCGTCCCGGGCAGTCGGACATCATGGGGACG
  GCCCCAGGCCCTGGCTGCGTGGCCAGAAGAAAAGCCTGGGCAAGAAAAGGGAGGAGTCGCTGGAGGCAAA
  GAGAAGGAAGCGGAGGTCCCGATCCTTCGAGGTCACCGGGCAAGGGCTCTCCCACCCCAAGACACACCTC
  CTGGGGCCCCATCAGGCGGAGCGCATCTCGGACCACAGGATGCCAGTGTGCAGGCACCCAGCCCCTGGTA
  TCCGGCATCTGGGAAAGGTCACGCTACCTTTGGCCAAAGTCAAACTCCCTCCAAGCCAGAACACGGGCCC
  GGGGGACTCCTCACCCCTGGCTGTTCCCCCCAACCCAGGTGGTGGTTCTCGACGGGCTACCCGTGGGCCC
  CGCCTGCCCCACGGCACAAGCACCCATGGCAAAGATGGATGCTCCCGGCATAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211178 representing NM_153209
  Red=Cloning site Green=Tags(s)
  
  MKDSGDSKDQQLMVALRVRPISVAELEEGATLIAHKVDEQMVVLMDPMEDPDDILRAHRSREKSYLFDVA
  FDFTATQEMVYQATTKSLIEGVISGYNATVFAYGPTGCGKTYTMLGTDQEPGIYVQTLNDLFRAIEETSN
  DMEYEVSMSYLEIYNEMIRDLLNPSLGYLELREDSKGVIQVAGITEVSTINAKEIMQLLMKGNRQRTQEP
  TAANQTSSRSHAVLQVTVRQRSRVKNILQEVRQGRLFMIDLAGSERASQTQNRGQRMKEGAHINRSLLAL
  GNCINALSDKGSNKYINYRDSKLTRLLKDSLGGNSRTVMIAHISPASSAFEESRNTLTYAGRAKNIKTRV
  KQNLLNVSYHIAQYTSIIADLRGEIQRLKRKIDEQTGRGQARGRQDRGDIRHIQAEVQLHSGQGEKAGMG
  QLREQLASAFQEQMDVRRRLLELENRAMEVQIDTSRHLLTIAGWKHEKSRRALKWREEQRKECYAKDDSE
  KDSDTGDDQPDILEPPEVAAARESIAALVDEQKQLRKQKLALEQRCRELRARGRRLEETLPRRIGSEEQR
  EVLSLLCRVHELEVENTEMQSHALLRDGALRHRHEAVRRLEQHRSLCDEIIQGQRQIIDDYNLAVPQRLE
  ELYEVYLRELEEGSLEQATIMDQVASRALQDSSLPKITPAGTSLTPDSDLESVKTLSSDAQHLQNSALPP
  LSTESEGHHVFKAGTGAWQAKSSSVPTPPPIQLGSLVTQEAPAQDSLGSWINSSPDSSENLSEIPLSHKE
  RKEILTGTKCIWVKAARRRSRALGTEGRHLLAPATERSSLSLHSLSEGDDARPPGPLACKRPPSPTLQHA
  ASEDNLSSSTGEAPSRAVGHHGDGPRPWLRGQKKSLGKKREESLEAKRRKRRSRSFEVTGQGLSHPKTHL
  LGPHQAERISDHRMPVCRHPAPGIRHLGKVTLPLAKVKLPPSQNTGPGDSSPLAVPPNPGGGSRRATRGP
  RLPHGTSTHGKDGCSRHN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_153209
• ORF Size: 2994 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153209.4
• RefSeq Size: 3670 bp
• RefSeq ORF: 2997 bp
• Locus ID: 124602
• UniProt ID: Q2TAC6
• Cytogenetics: 17q25.1
• Protein Families: Druggable Genome
• MW: 111.2 kDa
• Gene Summary: Plus end-directed microtubule-dependent motor protein that regulates the length of motile cilia by mediating depolymerization of microtubules at ciliary tips.[UniProtKB/Swiss-Prot Function]