IL1RAPL2 (NM_017416) Human Tagged ORF Clone

CAT#: RC211149

IL1RAPL2 (Myc-DDK-tagged)-Human interleukin 1 receptor accessory protein-like 2 (IL1RAPL2)

Product Images

Circular map for RC211149

• 

  Western blot validation of overexpression lysate (Cat# LY413774) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC211149 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: IL1RAPL2
• Synonyms: IL-1R9; IL1R9; IL1RAPL-2; TIGIRR-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211149 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGCCACCATTTCTTTTGGCCCTTGTGGTCTGTTCTGTAGTCAGCACAAATCTGAAGATGGTGTCAA
  AGAGAAATTCTGTGGATGGCTGCATTGACTGGTCAGTGGATCTCAAGACATACATGGCTTTGGCAGGTGA
  ACCAGTCCGAGTGAAATGTGCCCTTTTCTACAGTTATATTCGTACCAACTATAGCACGGCCCAGAGCACT
  GGGCTCAGGCTTATGTGGTACAAAAACAAAGGTGATTTGGAAGAGCCCATCATCTTTTCAGAGGTCAGGA
  TGAGCAAAGAGGAAGATTCAATATGGTTTCACTCAGCTGAGGCACAAGACAGTGGATTCTACACTTGTGT
  TTTAAGAAACTCAACATATTGCATGAAGGTGTCAATGTCCTTGACTGTTGCAGAGAATGAATCAGGCCTG
  TGCTACAACAGCAGGATCCGCTATTTAGAAAAATCTGAAGTCACTAAAAGAAAGGAGATCTCCTGTCCAG
  ACATGGATGACTTTAAAAAGTCCGATCAGGAGCCTGATGTTGTGTGGTATAAGGAATGCAAGCCAAAAAT
  GTGGAGAAGCATAATAATACAGAAAGGAAATGCTCTTCTGATCCAAGAAGTTCAAGAAGAAGATGGAGGA
  AATTACACATGTGAACTTAAATATGAAGGAAAACTTGTAAGACGAACAACTGAATTGAAAGTTACAGCTT
  TACTCACAGACAAGCCTCCCAAGCCATTGTTCCCCATGGAGAATCAGCCAAGTGTTATAGATGTCCAGCT
  GGGTAAGCCTCTGAACATCCCCTGCAAAGCATTCTTCGGATTCAGTGGAGAGTCTGGGCCAATGATCTAC
  TGGATGAAAGGAGAAAAGTTTATTGAAGAACTGGCAGGTCACATTAGAGAAGGTGAAATAAGGCTTCTCA
  AAGAGCATCTTGGAGAAAAAGAAGTTGAATTGGCACTCATCTTTGACTCAGTTGTGGAAGCTGACCTGGC
  GAATTATACCTGCCATGTTGAAAACCGAAATGGACGGAAACATGCCAGTGTTTTGCTGCGTAAAAAGGAT
  TTAATCTATAAAATTGAGCTTGCAGGGGGCCTGGGAGCAATCTTCCTCCTCCTTGTACTGCTGGTGGTCA
  TTTACAAATGCTACAACATTGAATTGATGCTCTTCTACAGGCAGCACTTTGGAGCTGATGAAACTAATGA
  TGACAACAAGGAATATGATGCCTATCTCTCTTACACAAAAGTGGACCAAGATACTTTAGACTGTGACAAT
  CCTGAAGAAGAGCAGTTTGCTCTTGAAGTACTGCCAGATGTCCTGGAAAAACACTATGGATATAAACTCT
  TCATCCCAGAAAGAGACCTGATTCCAAGTGGAACATACATGGAAGATCTCACAAGATATGTTGAACAAAG
  CAGAAGACTTATTATCGTGCTAACTCCAGACTATATTCTCAGACGGGGATGGAGTATTTTCGAACTGGAA
  AGCAGACTCCATAACATGCTAGTCAGTGGAGAAATCAAAGTGATTTTGATTGAGTGTACAGAATTAAAAG
  GGAAAGTGAATTGCCAGGAAGTGGAATCACTAAAGCGTAGCATCAAACTTCTGTCCCTGATCAAGTGGAA
  GGGATCCAAAAGCAGCAAATTAAATTCTAAGTTTTGGAAGCACTTAGTATATGAAATGCCCATCAAGAAA
  AAAGAAATGCTACCTCGGTGCCATGTTCTGGACTCCGCAGAACAAGGACTTTTTGGAGAACTCCAGCCTA
  TACCCTCTATTGCCATGACCAGTACTTCAGCCACTCTGGTGTCATCTCAGGCTGATCTCCCTGAATTCCA
  CCCTTCAGATTCAATGCAAATCAGGCACTGTTGCAGAGGTTATAAACATGAGATACCAGCCACGACCTTG
  CCAGTACCTTCCTTAGGCAACCACCATACTTATTGTAACCTGCCTCTGACGCTACTCAACGGACAGCTAC
  CCCTTAATAACACCCTGAAAGATACCCAGGAATTTCACAGGAACAGTTCTTTGCTGCCTTTATCCTCCAA
  AGAGCTTAGCTTTACCAGTGATATTTGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211149 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MKPPFLLALVVCSVVSTNLKMVSKRNSVDGCIDWSVDLKTYMALAGEPVRVKCALFYSYIRTNYSTAQST
  GLRLMWYKNKGDLEEPIIFSEVRMSKEEDSIWFHSAEAQDSGFYTCVLRNSTYCMKVSMSLTVAENESGL
  CYNSRIRYLEKSEVTKRKEISCPDMDDFKKSDQEPDVVWYKECKPKMWRSIIIQKGNALLIQEVQEEDGG
  NYTCELKYEGKLVRRTTELKVTALLTDKPPKPLFPMENQPSVIDVQLGKPLNIPCKAFFGFSGESGPMIY
  WMKGEKFIEELAGHIREGEIRLLKEHLGEKEVELALIFDSVVEADLANYTCHVENRNGRKHASVLLRKKD
  LIYKIELAGGLGAIFLLLVLLVVIYKCYNIELMLFYRQHFGADETNDDNKEYDAYLSYTKVDQDTLDCDN
  PEEEQFALEVLPDVLEKHYGYKLFIPERDLIPSGTYMEDLTRYVEQSRRLIIVLTPDYILRRGWSIFELE
  SRLHNMLVSGEIKVILIECTELKGKVNCQEVESLKRSIKLLSLIKWKGSKSSKLNSKFWKHLVYEMPIKK
  KEMLPRCHVLDSAEQGLFGELQPIPSIAMTSTSATLVSSQADLPEFHPSDSMQIRHCCRGYKHEIPATTL
  PVPSLGNHHTYCNLPLTLLNGQLPLNNTLKDTQEFHRNSSLLPLSSKELSFTSDIW
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017416
• ORF Size: 2058 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017416.2
• RefSeq Size: 2985 bp
• RefSeq ORF: 2061 bp
• Locus ID: 26280
• UniProt ID: Q9NP60
• Cytogenetics: Xq22.3
• Protein Families: Druggable Genome, Transmembrane
• MW: 78.7 kDa
• Gene Summary: The protein encoded by this gene is a member of the interleukin 1 receptor family. This protein is similar to the interleukin 1 accessory proteins, and is most closely related to interleukin 1 receptor accessory protein-like 1 (IL1RAPL1). This gene and IL1RAPL1 are located at a region on chromosome X that is associated with X-linked non-syndromic cognitive disability. [provided by RefSeq, Jul 2008]