SNAPC3 (NM_001039697) Human Tagged ORF Clone

CAT#: RC211133

SNAPC3 (Myc-DDK-tagged)-Human small nuclear RNA activating complex, polypeptide 3, 50kDa (SNAPC3)

Product Images

Circular map for RC211133

• 

  Western blot validation of overexpression lysate (Cat# LY421891) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC211133 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SNAPC3
• Synonyms: PTFbeta; SNAP50
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211133 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGAAGGAAGCCGAGGTGGCCCTACGTGTAGCGGGGTGGGTGGCAGGCAGGACCCAGTCTCCGGCA
  GTGGCGGCTGCAACTTTCCAGAGTATGAGCTTCCCGAGCTAAATACGCGCGCTTTCCATGTGGGCGCCTT
  TGGGGAGCTGTGGCGGGGCCGTCTGCGCGGGGCCGGGGACTTGTCGCTGAGGGAGCCGCCGGCATCCGCT
  CTGCCTGGGAGCCAGGCAGCTGACTCCGACCGGGAGGATGCCGCGGTGGCCAGGGATCTGGACTGCAGCC
  TGGAGGCGGCGGCTGAGCTGAGGGCGGTGTGCGGCCTTGATAAACTGAAATGCCTTGAGGACGGTGAGGA
  TCCAGAAGTCATTCCGGAGAATACTGACCTGGTGACTTTGGGGGTTAGAAAAAGGTTCTTGGAACATCGG
  GAAGAAACCATTACAATAGATCGAGCCTGCAGACAAGAAACATTCGTTTATGAGATGGAGTCACATGCCA
  TAGGAAAAAAGCCTGAAAATTCAGCAGACATGATTGAAGAAGGGGAGCTTATCCTATCTGTGAATATCTT
  GTACCCTGTTATATTTCATAAGCACAAAGAACACAAACCATACCAAACAATGCTGGTGTTGGGCAGTCAA
  AAACTCACACAACTGAGGGATTCAATTCGATGTGTCAGTGACCTCCAGATTGGTGGTGAATTCAGCAACA
  CTCCTGACCAAGCCCCTGAGCACATCAGCAAAGACCTATACAAATCAGCCTTCTTTTATTTTGAAGGAAC
  ATTTTATAATGATAAAAGATACCCAGAATGCAGAGATTTGAGCAGAACTATCATTGAGTGGTCAGAGTCC
  CATGATAGAGGCTATGGAAAGTTTCAGACTGCTAGAATGGAAGATTTCACCTTCAATGACTTGTGTATTA
  AACTGGGTTTTCCTTACTTATACTGTCATCAGGGAGACTGTGAACATGTCATTGTCATTACTGACATAAG
  GCTTGTGCATCATGATGACTGCTTGGATAGGACATTGTATCCCCTCCTTATCAAGAAGCATTGGCTATGG
  ACCAGAAAATGTTTTGTTTGTAAAATGTATACAGCCAGATGGGTGACGAACAATGACAGTTTTGCACCAG
  AGGACCCATGCTTCTTTTGTGATGTTTGCTTCCGAATGCTGCACTATGATTCAGAAGGCAACAAACTGGG
  GGAATTCCTTGCTTATCCTTATGTTGATCCTGGAACCTTTAAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211133 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAEGSRGGPTCSGVGGRQDPVSGSGGCNFPEYELPELNTRAFHVGAFGELWRGRLRGAGDLSLREPPASA
  LPGSQAADSDREDAAVARDLDCSLEAAAELRAVCGLDKLKCLEDGEDPEVIPENTDLVTLGVRKRFLEHR
  EETITIDRACRQETFVYEMESHAIGKKPENSADMIEEGELILSVNILYPVIFHKHKEHKPYQTMLVLGSQ
  KLTQLRDSIRCVSDLQIGGEFSNTPDQAPEHISKDLYKSAFFYFEGTFYNDKRYPECRDLSRTIIEWSES
  HDRGYGKFQTARMEDFTFNDLCIKLGFPYLYCHQGDCEHVIVITDIRLVHHDDCLDRTLYPLLIKKHWLW
  TRKCFVCKMYTARWVTNNDSFAPEDPCFFCDVCFRMLHYDSEGNKLGEFLAYPYVDPGTFN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001039697
• ORF Size: 1233 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001039697.1, NP_001034786.1
• RefSeq Size: 3095 bp
• RefSeq ORF: 1236 bp
• Locus ID: 6619
• UniProt ID: Q92966
• Cytogenetics: 9p22.3
• Protein Families: Transcription Factors
• MW: 46.8 kDa
• Gene Summary: Part of the SNAPc complex required for the transcription of both RNA polymerase II and III small-nuclear RNA genes. Binds to the proximal sequence element (PSE), a non-TATA-box basal promoter element common to these 2 types of genes. Recruits TBP and BRF2 to the U6 snRNA TATA box.[UniProtKB/Swiss-Prot Function]