gamma Tubulin (TUBG2) (NM_016437) Human Tagged ORF Clone

CAT#: RC211127

TUBG2 (Myc-DDK-tagged)-Human tubulin, gamma 2 (TUBG2)

Product Images

Circular map for RC211127

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  Western blot validation of overexpression lysate (Cat# LY414021) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC211127 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified TUBG2 protein (Cat# TP311127). The protein was produced from HEK293T cells transfected with TUBG2 cDNA clone (Cat# RC211127) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: gamma Tubulin
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211127 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCCGGGAGATCATCACCCTGCAGCTGGGCCAGTGCGGCAACCAGATTGGGTTCGAGTTCTGGAAAC
  AGCTGTGCGCCGAGCATGGTATCAGCCCCGAGGGCATCGTGGAGGAATTCGCCACCGAGGGCACTGACCG
  CAAGGACGTCTTTTTCTACCAGGCAGACGATGAGCACTACATCCCCCGGGCCGTGCTGCTGGACTTGGAA
  CCCCGGGTGATCCACTCCATCCTCAACTCCCCCTATGCCAAGCTCTACAACCCAGAGAACATCTACCTGT
  CGGAACATGGAGGAGGAGCTGGCAACAACTGGGCCAGCGGATTCTCCCAGGGTGAGAAAATTCATGAAGA
  CATCTTTGACATCATAGACCGAGAAGCAGATGGAAGTGACAGTTTGGAGGGCTTCGTGCTGTGTCACTCC
  ATCGCTGGGGGTACGGGTTCTGGCCTGGGCTCCTACCTCCTGGAGCGACTGAATGACAGGTACCCCAAGA
  AGCTAGTGCAGACTTATTCAGTGTTTCCCTACCAGGACGAGATGAGCGACGTAGTGGTTCAGCCCTACAA
  TTCACTCCTGACACTCAAGAGGCTGACGCAGAACGCAGATTGTGTGGTGGTGCTGGACAACACAGCCCTG
  AACCGGATTGCCACAGACCGCCTGCACATCCAGAACCCGTCCTTCTCCCAGATCAACCAGCTGGTGTCCA
  CCATCATGTCGGCCAGCACCACCACCCTGCGCTACCCCGGCTACATGAACAATGACCTCATCGGCCTCAT
  CGCCTCGCTCATTCCCACCCCACGGCTCCACTTCCTCATGACCGGCTACACCCCGCTCACTACAGACCAG
  TCAGTGGCCAGCGTGAGGAAGACCACGGTCCTGGATGTCATGAGGCGGCTGCTGCAGCCCAAGAACGTGA
  TGGTGTCCACAGGCCGAGACCGCCAGACCAACCACTGCTACATCGCCATCCTCAACATCATCCAGGGAGA
  GGTGGACCCCACCCAGGTCCACAAGAGCCTGCAGAGGATCCGGGAACGGAAGTTGGCCAACTTCATCCCG
  TGGGGCCCCGCCAGCATCCAGGTGGCCCTGTCGAGGAAGTCTCCCTACCTGCCCTCGGCCCACCGGGTCA
  GCGGGCTCATGATGGCCAACCACACCAGCATCTCCTCGCTCTTTGAAAGTTCCTGCCAGCAGTTTGACAA
  GCTGCGGAAGCGGGATGCCTTCCTCGAGCAGTTCCGTAAGGAGGACATGTTCAAGGACAACTTTGATGAG
  ATGGACAGGTCTAGGGAGGTTGTTCAGGAGCTCATTGATGAGTACCATGCGGCCACCCAGCCAGACTACA
  TTTCCTGGGGCACCCAGGAGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211127 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MPREIITLQLGQCGNQIGFEFWKQLCAEHGISPEGIVEEFATEGTDRKDVFFYQADDEHYIPRAVLLDLE
  PRVIHSILNSPYAKLYNPENIYLSEHGGGAGNNWASGFSQGEKIHEDIFDIIDREADGSDSLEGFVLCHS
  IAGGTGSGLGSYLLERLNDRYPKKLVQTYSVFPYQDEMSDVVVQPYNSLLTLKRLTQNADCVVVLDNTAL
  NRIATDRLHIQNPSFSQINQLVSTIMSASTTTLRYPGYMNNDLIGLIASLIPTPRLHFLMTGYTPLTTDQ
  SVASVRKTTVLDVMRRLLQPKNVMVSTGRDRQTNHCYIAILNIIQGEVDPTQVHKSLQRIRERKLANFIP
  WGPASIQVALSRKSPYLPSAHRVSGLMMANHTSISSLFESSCQQFDKLRKRDAFLEQFRKEDMFKDNFDE
  MDRSREVVQELIDEYHAATQPDYISWGTQEQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016437
• ORF Size: 1353 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016437.3
• RefSeq Size: 1841 bp
• RefSeq ORF: 1356 bp
• Locus ID: 27175
• UniProt ID: Q9NRH3
• Cytogenetics: 17q21.2
• Domains: tubulin
• Protein Families: Druggable Genome
• MW: 51.1 kDa
• Gene Summary: Tubulin is the major constituent of microtubules. The gamma chain is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta chain minus-end nucleation, centrosome duplication and spindle formation (By similarity).[UniProtKB/Swiss-Prot Function]