Apolipoprotein A V (APOA5) (NM_052968) Human Tagged ORF Clone

CAT#: RC211023

APOA5 (Myc-DDK-tagged)-Human apolipoprotein A-V (APOA5), transcript variant 1

Product Images

Circular map for RC211023

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY APOA5 (Cat# RC211023, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-APOA5(Cat# TA507102). Positive lysates LY403279 (100ug) and LC403279 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY403279) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC211023 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified APOA5 protein (Cat# TP311023). The protein was produced from HEK293T cells transfected with APOA5 cDNA clone (Cat# RC211023) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Apolipoprotein A V
• Synonyms: APOAV; RAP3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211023 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAAGCATGGCTGCCGTGCTCACCTGGGCTCTGGCTCTTCTTTCAGCGTTTTCGGCCACCCAGGCAC
  GGAAAGGCTTCTGGGACTACTTCAGCCAGACCAGCGGGGACAAAGGCAGGGTGGAGCAGATCCATCAGCA
  GAAGATGGCTCGCGAGCCCGCGACCCTGAAAGACAGCCTTGAGCAAGACCTCAACAATATGAACAAGTTC
  CTGGAAAAGCTGAGGCCTCTGAGTGGGAGCGAGGCTCCTCGGCTCCCACAGGACCCGGTGGGCATGCGGC
  GGCAGCTGCAGGAGGAGTTGGAGGAGGTGAAGGCTCGCCTCCAGCCCTACATGGCAGAGGCGCACGAGCT
  GGTGGGCTGGAATTTGGAGGGCTTGCGGCAGCAACTGAAGCCCTACACGATGGATCTGATGGAGCAGGTG
  GCCCTGCGCGTGCAGGAGCTGCAGGAGCAGTTGCGCGTGGTGGGGGAAGACACCAAGGCCCAGTTGCTGG
  GGGGCGTGGACGAGGCTTGGGCTTTGCTGCAGGGACTGCAGAGCCGCGTGGTGCACCACACCGGCCGCTT
  CAAAGAGCTCTTCCACCCATACGCCGAGAGCCTGGTGAGCGGCATCGGGCGCCACGTGCAGGAGCTGCAC
  CGCAGTGTGGCTCCGCACGCCCCCGCCAGCCCCGCGCGCCTCAGTCGCTGCGTGCAGGTGCTCTCCCGGA
  AGCTCACGCTCAAGGCCAAGGCCCTGCACGCACGCATCCAGCAGAACCTGGACCAGCTGCGCGAAGAGCT
  CAGCAGAGCCTTTGCAGGCACTGGGACTGAGGAAGGGGCCGGCCCGGACCCCCAGATGCTCTCCGAGGAG
  GTGCGCCAGCGACTTCAGGCTTTCCGCCAGGACACCTACCTGCAGATAGCTGCCTTCACTCGCGCCATCG
  ACCAGGAGACTGAGGAGGTCCAGCAGCAGCTGGCGCCACCTCCACCAGGCCACAGTGCCTTCGCCCCAGA
  GTTTCAACAAACAGACAGTGGCAAGGTTCTGAGCAAGCTGCAGGCCCGTCTGGATGACCTGTGGGAAGAC
  ATCACTCACAGCCTTCATGACCAGGGCCACAGCCATCTGGGGGACCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211023 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MASMAAVLTWALALLSAFSATQARKGFWDYFSQTSGDKGRVEQIHQQKMAREPATLKDSLEQDLNNMNKF
  LEKLRPLSGSEAPRLPQDPVGMRRQLQEELEEVKARLQPYMAEAHELVGWNLEGLRQQLKPYTMDLMEQV
  ALRVQELQEQLRVVGEDTKAQLLGGVDEAWALLQGLQSRVVHHTGRFKELFHPYAESLVSGIGRHVQELH
  RSVAPHAPASPARLSRCVQVLSRKLTLKAKALHARIQQNLDQLREELSRAFAGTGTEEGAGPDPQMLSEE
  VRQRLQAFRQDTYLQIAAFTRAIDQETEEVQQQLAPPPPGHSAFAPEFQQTDSGKVLSKLQARLDDLWED
  ITHSLHDQGHSHLGDP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_052968
• ORF Size: 1098 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_052968.5
• RefSeq Size: 1954 bp
• RefSeq ORF: 1101 bp
• Locus ID: 116519
• UniProt ID: Q6Q788
• Cytogenetics: 11q23.3
• Domains: Apolipoprotein
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: PPAR signaling pathway
• MW: 41.2 kDa
• Gene Summary: The protein encoded by this gene is an apolipoprotein that plays an important role in regulating the plasma triglyceride levels, a major risk factor for coronary artery disease. It is a component of high density lipoprotein and is highly similar to a rat protein that is upregulated in response to liver injury. Mutations in this gene have been associated with hypertriglyceridemia and hyperlipoproteinemia type 5. This gene is located proximal to the apolipoprotein gene cluster on chromosome 11q23. Alternatively spliced transcript variants encoding the same protein have been identified. [provided by RefSeq, Oct 2009]