PUS10 (NM_144709) Human Tagged ORF Clone

CAT#: RC210979

PUS10 (Myc-DDK-tagged)-Human pseudouridylate synthase 10 (PUS10)

Product Images

Circular map for RC210979

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  Western blot validation of overexpression lysate (Cat# LY408160) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC210979 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified PUS10 protein (Cat# TP310979). The protein was produced from HEK293T cells transfected with PUS10 cDNA clone (Cat# RC210979) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PUS10
• Synonyms: CCDC139; DOBI; Hup10
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210979 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCCCACTGACTGAGGAAAACAAGCATGTGGCCCAGTTGTTGCTCAATACTGGTACTTGTCCAAGAT
  GTATCTTCAGATTCTGTGGTGTGGATTTTCATGCACCTTACAAACTTCCATACAAGGAGTTGCTCAATGA
  ACTACAGAAATTTCTGGAAACTGAAAAAGATGAATTAATTTTGGAAGTTATGAACCCACCTCCCAAGAAA
  ATTCGACTGCAAGAACTGGAAGATAGTATTGATAATCTAAGTCAAAATGGAGAGGGAAGGATCTCTGTTA
  GTCATGTTGGAAGCACTGCTTCCAAGAACTCAAATTTAAATGTATGTAATGTATGCCTAGGAATTCTTCA
  AGAATTCTGTGAGAAAGATTTCATTAAAAAGGTGTGCCAAAAGGTTGAGGCCTCTGGGTTTGAATTCACC
  AGCTTGGTATTTTCAGTCTCCTTCCCACCACAACTATCTGTAAGAGAGCATGCTGCATGGTTGCTGGTAA
  AACAGGAAATGGGAAAGCAGAGTCTGTCGCTGGGAAGAGATGATATAGTTCAGCTAAAAGAAGCCTACAA
  ATGGATAACTCACCCCCTGTTTTCAGAGGAACTGGGTGTTCCCATTGATGGAAAGAGCTTGTTTGAAGTG
  AGTGTGGTCTTTGCTCACCCAGAAACAGTTGAGGATTGCCACTTCCTAGCTGCGATATGCCCAGATTGTT
  TTAAGCCAGCCAAAAACAAACAGTCTGTATTCACTAGAATGGCAGTTATGAAAGCCTTGAATAAGATAAA
  GGAAGAGGATTTCCTTAAGCAGTTTCCTTGTCCTCCAAACTCACCAAAGGCTGTATGCGCTGTTCTTGAA
  ATTGAATGTGCTCATGGTGCTGTTTTTGTGGCTGGGAGATATAATAAATACTCCAGGAATCTACCACAAA
  CTCCTTGGATAATTGATGGAGAAAGGAAGCTGGAATCTTCAGTGGAAGAATTAATTTCAGATCATCTGTT
  GGCAGTATTTAAAGCAGAGAGTTTTAATTTTTCATCCTCTGGAAGAGAAGATGTAGATGTGAGAACATTA
  GGAAATGGAAGGCCCTTTGCAATTGAGCTGGTGAATCCTCATAGAGTACATTTCACTTCACAAGAAATTA
  AGGAACTTCAGCAGAAAATTAATAACTCATCTAACAAAATCCAAGTACGTGACTTGCAGCTTGTCACAAG
  AGAGGCAATAGGACATATGAAAGAAGGTGAAGAAGAAAAGACAAAGACCTACAGTGCCTTAATTTGGACA
  AATAAAGCGATACAGAAGAAAGACATTGAATTCCTAAATGACATAAAGGACTTAAAAATCGACCAGAAAA
  CACCTTTGCGCGTCCTTCACCGAAGGCCCCTGGCTGTGCGAGCTCGCGTCATTCACTTCATGGAGACACA
  GTACGTGGATGAGCACCACTTCCGCCTCCACTTGAAAACTCAGGCTGGCACCTACATTAAAGAGTTTGTA
  CATGGAGACTTCGGGAGAACCAAGCCAAACATTGGGTCCCTGATGAATGTGACTGCAGACATTCTGGAGC
  TGGATGTTGAGTCTGTAGATGTTGACTGGCCACCTGCTCTGGATGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210979 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MFPLTEENKHVAQLLLNTGTCPRCIFRFCGVDFHAPYKLPYKELLNELQKFLETEKDELILEVMNPPPKK
  IRLQELEDSIDNLSQNGEGRISVSHVGSTASKNSNLNVCNVCLGILQEFCEKDFIKKVCQKVEASGFEFT
  SLVFSVSFPPQLSVREHAAWLLVKQEMGKQSLSLGRDDIVQLKEAYKWITHPLFSEELGVPIDGKSLFEV
  SVVFAHPETVEDCHFLAAICPDCFKPAKNKQSVFTRMAVMKALNKIKEEDFLKQFPCPPNSPKAVCAVLE
  IECAHGAVFVAGRYNKYSRNLPQTPWIIDGERKLESSVEELISDHLLAVFKAESFNFSSSGREDVDVRTL
  GNGRPFAIELVNPHRVHFTSQEIKELQQKINNSSNKIQVRDLQLVTREAIGHMKEGEEEKTKTYSALIWT
  NKAIQKKDIEFLNDIKDLKIDQKTPLRVLHRRPLAVRARVIHFMETQYVDEHHFRLHLKTQAGTYIKEFV
  HGDFGRTKPNIGSLMNVTADILELDVESVDVDWPPALDD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_144709
• ORF Size: 1587 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_144709.4
• RefSeq Size: 3820 bp
• RefSeq ORF: 1590 bp
• Locus ID: 150962
• UniProt ID: Q3MIT2
• Cytogenetics: 2p16.1-p15
• MW: 60.2 kDa
• Gene Summary: Pseudouridination, the isomerization of uridine to pseudouridine, is the most common posttranscriptional nucleotide modification found in RNA and is essential for biologic functions such as spliceosome biogenesis. Pseudouridylate synthases, such as PUS10, catalyze pseudouridination of structural RNAs, including transfer, ribosomal, and splicing RNAs. These enzymes also act as RNA chaperones, facilitating the correct folding and assembly of tRNAs (McCleverty et al., 2007 [PubMed 17900615]).[supplied by OMIM, May 2009]