LONRF2 (NM_198461) Human Tagged ORF Clone

CAT#: RC210975

LONRF2 (Myc-DDK-tagged)-Human LON peptidase N-terminal domain and ring finger 2 (LONRF2)

Product Images

Circular map for RC210975

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: LONRF2
• Synonyms: RNF192
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210975 representing NM_198461
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCCCCGAGCCGGTCCCGCCGCCGCCGCCGCCCCAGTGTCCTGGCTGCGACCGCGCGGAGCCGATCG
  CCCAGCGCTTAGAGGAGGGCGACGAGGCCTTCCGCGCGGGCGACTACGAGATGGCAGCCGAGCTCTTTCG
  CTCCATGCTAGCCGGGCTGGCGCAGCCGGACCGCGGCCTGTGCCTGAGGCTGGGGGACGCGCTGGCCCGC
  GCCGGCCGCCTCCCCGAAGCCCTGGGCGCGTTCCGCGGCGCCGCGCGGCTCGGGGCGCTGCGGCCGGAGG
  AGCTGGAAGAGCTGGCGGGCGGCCTGGTGCGCGCCGTGGGCCTGCGCGACCGGCCGCTGTCCGCGGAGAA
  CCCGGGCGGCGAGCCCGAGGCGCCCGGCGAGGGAGGGCCGGCCCCGGAGCCCCGCGCGCCCCGCGACCTG
  CTCGGCTGCCCGCGCTGCCGGCGGCTGCTGCATAAGCCGGTGACGCTGCCCTGCGGGCTCACAGTCTGCA
  AGCGCTGCGTGGAGCCAGGGCCCGCGCGGCCGCAGGTGCGGCGCGTGAACGTGGTGCTGAGCGGCCTGCT
  GGAGAAGTGCTTCCCGGCCGAGTGCCGGCTGCGCAGGCTGGCAGGCCAGGCGCGGAGCCTGCAGCGCCAG
  CAGCAGCCGGAGGCCGCGCTGCTCAGGTGCGACCAGGCCCTGGAGCTGGCTCCTGATGATAATTCATTAT
  TGCTGCTGCGGGCGGAGTTATATTTGACCATGAAGAACTATGAGCAAGCTCTCCAAGATGCCAGTGCAGC
  TTGTCAGAATGAACCTCTCTTGATTAAGGGACATCAAGTAAAAGCTCAGGCTCTTTCTGGATTGGGAAGA
  AGTAAGGAAGTGTTAAAGGAATTTCTCTACTGCCTTGCTCTGAATCCTGAATGTAACTCTGTGAAGAAAG
  AAGCACAGAAGGTAATGTGTGAAGTGCTGTTTTCAGCTACAGCAAATGTGCATGAAAATTTAACATCTTC
  CATCCAAAGCAGATTAAAGGCTCAGGGTCACAGCCACATGAATGCCCAGGCTCTGCTGGAAGAAGGTGAT
  GCAGGGAGCTCGGAGAATTCATCTGAGAAATCTGATATGCTTGGAAATACCAATTCTTCAGTTTTGTATT
  TTATACTGGGTCTACACTTTGAAGAGGATAAAAAGGCGTTAGAAAGCATCCTTCCAACAGCACCCAGCGC
  TGGCTTAAAGAGACAGTTTCCGGATGACGTGGAGGATGCACCTGACCTGAACGCCCCTGGAAAAATTCCC
  AAGAAAGATCTCTCACTTCAAAGGAGCCCAAACTCTGAGACAGAAGAAAGTCAGGGGCTCTCGCTTGATG
  TAACTGACTTTGAGTGTGCCCTCTGCATGAGATTGCTCTTTGAACCTGTCACTACGCCCTGTGGACACAC
  ATTTTGCCTAAAATGCCTTGAGCGCTGCCTTGACCACGCCCCACACTGTCCTTTGTGCAAAGACAAACTT
  TCGGAATTATTGGCAAGCAGAAACTTTAACATAACTGTTCTGGCCGAAGAATTAATATTTCGATATTTGC
  CGGATGAATTGTCTGATAGGAAGAGAATTTATGATGAAGAAATGTCAGAACTGTCAAATCTGACCAGAGA
  CGTCCCCATCTTTGTGTGTGCCATGGCCTTCCCCACGGTCCCATGTCCACTCCACGTTTTTGAGCCCCGC
  TATCGGCTTATGATAAGAAGATGCATGGAAACTGGCACCAAGCGGTTTGGCATGTGTTTATCTGCTGAGC
  ACGCGGGGCTTTCAGAGTATGGATGCATGCTGGAGATTAAGGACGTGAGAACGTTTCCTGATGGAAGTTC
  TGTTGTAGACGCGATTGGCATCAGTCGGTTCCGAGTGCTAAGCCACCGCCACAGAGATGGCTATAACACA
  GCGGACATTGAATATCTTGAAGATGAAAAGGTGGAGGGTCCAGAGTATGAAGAACTTGCCGCTCTCCACG
  ATTCTGTGCATCAACAGTCTGTTTCCTGGTTCGCGTCTCTCCAGGATCGCATGAAAGAACAAATTTTAAG
  TCATTTTGGGGTAATGCCAGACAGAGAACCTGAGCCTCAGAGTAATCCCAGCGGCCCTGCCTGGTCCTGG
  TGGATCCTGGCCGTGCTGCCCCTGGAGCGCAAGGCTCAGCTGGCCATCCTCGGCATGACCTCGCTCAAAG
  AGCGGCTCCTTGCCATCCGACGGATATTAGTCATCATCACGCGTAAGATGAATAGTCGGCAAGAGCTGGC
  TAATGCCAGGGAGAGAAATAAT
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210975 representing NM_198461
  Red=Cloning site Green=Tags(s)
  
  MSPEPVPPPPPPQCPGCDRAEPIAQRLEEGDEAFRAGDYEMAAELFRSMLAGLAQPDRGLCLRLGDALAR
  AGRLPEALGAFRGAARLGALRPEELEELAGGLVRAVGLRDRPLSAENPGGEPEAPGEGGPAPEPRAPRDL
  LGCPRCRRLLHKPVTLPCGLTVCKRCVEPGPARPQVRRVNVVLSGLLEKCFPAECRLRRLAGQARSLQRQ
  QQPEAALLRCDQALELAPDDNSLLLLRAELYLTMKNYEQALQDASAACQNEPLLIKGHQVKAQALSGLGR
  SKEVLKEFLYCLALNPECNSVKKEAQKVMCEVLFSATANVHENLTSSIQSRLKAQGHSHMNAQALLEEGD
  AGSSENSSEKSDMLGNTNSSVLYFILGLHFEEDKKALESILPTAPSAGLKRQFPDDVEDAPDLNAPGKIP
  KKDLSLQRSPNSETEESQGLSLDVTDFECALCMRLLFEPVTTPCGHTFCLKCLERCLDHAPHCPLCKDKL
  SELLASRNFNITVLAEELIFRYLPDELSDRKRIYDEEMSELSNLTRDVPIFVCAMAFPTVPCPLHVFEPR
  YRLMIRRCMETGTKRFGMCLSAEHAGLSEYGCMLEIKDVRTFPDGSSVVDAIGISRFRVLSHRHRDGYNT
  ADIEYLEDEKVEGPEYEELAALHDSVHQQSVSWFASLQDRMKEQILSHFGVMPDREPEPQSNPSGPAWSW
  WILAVLPLERKAQLAILGMTSLKERLLAIRRILVIITRKMNSRQELANARERNN
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_198461
• ORF Size: 2262 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_198461.4
• RefSeq Size: 13920 bp
• RefSeq ORF: 2265 bp
• Locus ID: 164832
• UniProt ID: Q1L5Z9
• Cytogenetics: 2q11.2
• Protein Families: Druggable Genome
• MW: 84.1 kDa