GRASP1 (GRIPAP1) (NM_020137) Human Tagged ORF Clone

CAT#: RC210936

GRIPAP1 (Myc-DDK-tagged)-Human GRIP1 associated protein 1 (GRIPAP1), transcript variant 1

Product Images

Circular map for RC210936

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY GRIPAP1 (Cat# RC210936, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GRIPAP1(Cat# TA500876). Positive lysates LY402754 (100ug) and LC402754 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY402754) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC210936 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified GRIPAP1 protein (Cat# TP310936). The protein was produced from HEK293T cells transfected with GRIPAP1 cDNA clone (Cat# RC210936) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GRASP1
• Synonyms: GRASP-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210936 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCAAGCTCTGTCTGAGGAGGAGTTTCAGCGGATGCAGGCTCAGCTCCTGGAACTCCGGACAAACA
  ACTACCAGCTTTCAGATGAACTACGCAAGAATGGTGTTGAACTCACCAGTCTTCGACAGAAGGTCGCCTA
  CTTGGATAAGGAGTTCAGCAAAGCTCAGAAGGCACTGAGCAAGAGCAAGAAAGCTCAGGAAGTCGAGGTA
  TTGCTGAGTGAAAATGAGATGCTGCAGGCAAAGCTGCACAGCCAGGAGGAGGACTTCCGTTTGCAGAACA
  GCACACTAATGGCCGAGTTCAGCAAGCTCTGCAGCCAGATGGAACAGCTGGAGCAAGAGAACCAGCAACT
  GAAGGAGGGGGCTGCAGGAGCAGGGGTTGCCCAAGCTGGGCCCCTCGTGGATGGGGAGCTGCTGAGGCTA
  CAGGCTGAAAACACAGCCTTGCAGAAGAACGTGGCAGCCCTGCAGGAACGCTATGGGAAAGAAGCCGGGA
  AGTTCTCAGCTGTCAGTGAGGGCCAGGGGGATCCCCCAGGGGGCCCGGCCCCCACCGTCCTGGCCCCCAT
  GCCGTTGGCAGAGGTGGAGCTGAAATGGGAAATGGAGAAAGAGGAGAAGAGATTGCTCTGGGAACAGCTG
  CAAGGCTTAGAGAGCTCAAAGCAGGCCGAAACATCCAGGCTGCAGGAGGAACTTGCTAAGCTCTCCGAGA
  AACTGAAAAAGAAACAAGAAAGTTTTTGCCGTCTGCAGACAGAAAAGGAGACTCTGTTTAATGACAGCAG
  GAACAAGATTGAGGAATTACAACAACGGAAGGAAGCTGATCACAAAGCCCAGTTGGCTCGAACCCAGAAG
  CTGCAGCAGGAACTTGAGGCTGCCAATCAGAGCTTGGCAGAGCTGAGAGATCAGCGGCAGGGGGAGCGCC
  TGGAACATGCAGCAGCTTTGCGGGCCCTACAAGATCAGGTATCCATCCAGAGTGCAGATGCACAGGAACA
  AGTGGAAGGGCTTTTGGCTGAGAACAATGCCTTGAGGACTAGCCTGGCTGCCCTGGAGCAGATCCAAACA
  GCAAAGACCCAAGAACTGAATATGCTCCGGGAACAGACCACTGGGCTGGCAGCTGAGTTGCAGCAGCAGC
  AGGCTGAGTACGAGGACCTTATGGGACAGAAAGATGACCTCAACTCCCAGCTCCAGGAGTCATTACGGGC
  CAATAGTCGACTGCTGGAACAACTTCAAGAAATAGGGCAGGAGAAGGAGCAGTTGACCCAGGAATTACAG
  GAGGCTCGGAAGAGTGCGGAGAAGCGGAAGGCCATGCTGGATGAGCTAGCAATGGAAACGCTGCAAGAGA
  AGTCCCAGCACAAGGAAGAGCTGGGAGCAGTTCGTCTACGGCATGAGAAGGAGGTGCTGGGGGTGCGTGC
  CCGCTATGAGCGTGAGCTCCGAGAGCTGCATGAAGACAAGAAGCGTCAGGAGGAGGAGCTCCGTGGGCAG
  ATCCGGGAGGAGAAGGCCCGGACACGGGAGCTGGAGACTCTCCAGCAGACAGTGGAAGAACTTCAAGCTC
  AGGTACATTCCATGGATGGAGCCAAGGGCTGGTTTGAACGGCGCTTGAAGGAAGCCGAGGAATCCCTGCA
  GCAGCAGCAGCAGGAACAAGAGGAAGCCCTCAAGCAGTGTCGGGAGCAGCACGCTGCCGAGCTGAAGGGC
  AAGGAGGAGGAGCTACAGGATGTACGGGATCAGCTCGAGCAGGCCCAGGAGGAGCGGGACTGCCACCTGA
  AGACCATTAGCAGCCTGAAGCAGGAGGTGAAGGACACAGTGGATGGGCAGAGGATCCTGGAGAAGAAGGG
  CAGTGCTGCGCTCAAGGACCTCAAGCGGCAGCTGCATTTGGAGCGGAAACGGGCAGATAAGCTGCAGGAG
  CGACTGCAGGACATCCTCACTAACAGCAAGAGCCGCTCAGGCCTTGAGGAGCTGGTTCTCTCAGAGATGA
  ACTCACCAAGCCGGACCCAGACAGGGGACAGCAGTAGCATCTCCTCCTTCAGCTACCGGGAGATCTTGCG
  GGAAAAGGAGAGCTCGGCTGTTCCAGCCAGGTCCTTATCCAGCAGCCCTCAAGCCCAGCCCCCTCGGCCA
  GCAGAGCTGTCAGATGAGGAAGTGGCTGAGCTCTTTCAGCGGCTGGCAGAGACACAGCAGGAGAAATGGA
  TGCTGGAGGAGAAGGTGAAGCACCTGGAAGTGAGCAGTGCTTCCATGGCAGAGGACCTCTGCCGGAAGAG
  CGCCATCATTGAGACCTACGTCATGGACAGCCGGATCGATGTGTCTGTGGCAGCAGGCCACACAGACCGC
  AGCGGGCTGGGCAGCGTCCTGAGAGACCTAGTGAAGCCAGGCGACGAGAACCTTCGGGAGATGAACAAGA
  AGCTGCAGAACATGCTGGAGGAGCAGCTCACCAAGAATATGCACTTGCACAAGGATATGGAAGTTCTGTC
  CCAGGAAATTGTGCGGCTCAGCAAGGAGTGCGTGGGGCCTCCTGACCCAGACCTAGAGCCAGGAGAAACC
  AGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210936 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAQALSEEEFQRMQAQLLELRTNNYQLSDELRKNGVELTSLRQKVAYLDKEFSKAQKALSKSKKAQEVEV
  LLSENEMLQAKLHSQEEDFRLQNSTLMAEFSKLCSQMEQLEQENQQLKEGAAGAGVAQAGPLVDGELLRL
  QAENTALQKNVAALQERYGKEAGKFSAVSEGQGDPPGGPAPTVLAPMPLAEVELKWEMEKEEKRLLWEQL
  QGLESSKQAETSRLQEELAKLSEKLKKKQESFCRLQTEKETLFNDSRNKIEELQQRKEADHKAQLARTQK
  LQQELEAANQSLAELRDQRQGERLEHAAALRALQDQVSIQSADAQEQVEGLLAENNALRTSLAALEQIQT
  AKTQELNMLREQTTGLAAELQQQQAEYEDLMGQKDDLNSQLQESLRANSRLLEQLQEIGQEKEQLTQELQ
  EARKSAEKRKAMLDELAMETLQEKSQHKEELGAVRLRHEKEVLGVRARYERELRELHEDKKRQEEELRGQ
  IREEKARTRELETLQQTVEELQAQVHSMDGAKGWFERRLKEAEESLQQQQQEQEEALKQCREQHAAELKG
  KEEELQDVRDQLEQAQEERDCHLKTISSLKQEVKDTVDGQRILEKKGSAALKDLKRQLHLERKRADKLQE
  RLQDILTNSKSRSGLEELVLSEMNSPSRTQTGDSSSISSFSYREILREKESSAVPARSLSSSPQAQPPRP
  AELSDEEVAELFQRLAETQQEKWMLEEKVKHLEVSSASMAEDLCRKSAIIETYVMDSRIDVSVAAGHTDR
  SGLGSVLRDLVKPGDENLREMNKKLQNMLEEQLTKNMHLHKDMEVLSQEIVRLSKECVGPPDPDLEPGET
  S
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020137
• ORF Size: 2523 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020137.3, NP_064522.3
• RefSeq Size: 3052 bp
• RefSeq ORF: 2526 bp
• Locus ID: 56850
• UniProt ID: Q4V328
• Cytogenetics: Xp11.23
• Protein Families: Druggable Genome
• MW: 96 kDa
• Gene Summary: This gene encodes a guanine nucleotide exchange factor for the Ras family of small G proteins (RasGEF). The encoded protein interacts in a complex with glutamate receptor interacting protein 1 (GRIP1) and plays a role in the regulation of AMPA receptor function. [provided by RefSeq, Aug 2013]