MSL2L1 (MSL2) (NM_018133) Human Tagged ORF Clone

CAT#: RC210829

MSL2 (Myc-DDK-tagged)-Human male-specific lethal 2 homolog (Drosophila) (MSL2), transcript variant 1

Product Images

Circular map for RC210829

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY MSL2 (Cat# RC210829, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MSL2 (Cat# TA811181)(1:2000). Positive lysates LY413286 (100ug) and LC413286 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY413286) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC210829 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MSL2L1
• Synonyms: MSL-2; MSL2L1; RNF184
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210829 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACCCCGTGAATGCTACTGCTCTCTACATTTCCGCGAGCCGCCTAGTGCTCAACTACGACCCCGGAG
  ACCCCAAGGCGTTTACTGAGATTAACAGGCTCTTGCCTTACTTCCGACAGTCCCTTTCGTGCTGTGTTTG
  CGGACATTTGCTACAAGATCCTATTGCACCCACCAACTCCACCTGCCAACATTATGTCTGCAAAACTTGT
  AAAGGCAAGAAAATGATGATGAAACCTTCCTGTAGCTGGTGCAAAGACTATGAGCAGTTTGAGGAAAACA
  AGCAGTTAAGCATCCTAGTGAACTGCTACAAAAAACTATGCGAGTATATAACACAGACTACACTGGCACG
  GGATATAATAGAAGCAGTTGACTGTTCTTCTGATATTTTGGCTTTGCTTAATGATGGATCATTGTTTTGT
  GAGGAGACAGAAAAACCCTCAGATTCATCCTTTACTTTGTGTTTGACACATTCCCCTTTACCTTCAACCT
  CAGAACCCACAACTGATCCTCAAGCTAGTTTATCTCCAATGTCTGAAAGCACCCTCAGCATTGCTATTGG
  CAGTTCTGTTATCAATGGTTTGCCTACTTATAATGGGCTTTCAATAGATAGATTTGGTATAAATATTCCT
  TCACCTGAACATTCAAATACGATTGACGTATGTAATACTGTTGACATAAAAACTGAGGATCTGTCTGACA
  GCCTGCCACCCGTTTGTGACACAGTAGCCACTGACTTATGTTCCACAGGCATTGATATCTGCAGTTTCAG
  TGAAGATATAAAACCTGGAGACTCTCTGTTACTGAGTGTTGAGGAAGTACTCCGCAGCTTAGAAACTGTT
  TCAAATACAGAGGTCTGTTGCCCTAATTTGCAGCCGAACTTGGAAGCCACTGTATCCAATGGACCTTTTC
  TGCAGCTTTCTTCCCAGTCTCTTAGCCATAATGTTTTTATGTCCACCAGTCCTGCACTTCATGGGTTATC
  ATGTACAGCAGCAACTCCGAAGATAGCAAAATTGAATAGAAAACGATCCAGATCAGAGAGTGACAGTGAG
  AAAGTTCAGCCACTTCCAATTTCTACCATTATCCGAGGCCCAACACTGGGGGCATCTGCTCCTGTGACAG
  TGAAACGGGAGAGCAAAATTTCTCTTCAACCTATAGCAACTGTTCCCAATGGAGGCACAACACCTAAAAT
  CAGCAAAACTGTACTTTTATCTACTAAAAGCATGAAAAAGAGTCATGAACATGGATCCAAGAAATCTCAC
  TCTAAAACCAAGCCAGGTATTCTTAAAAAAGACAAAGCAGTAAAGGAAAAGATTCCTAGTCATCATTTTA
  TGCCAGGAAGTCCTACCAAGACTGTGTACAAAAAACCCCAGGAAAAGAAAGGGTGTAAATGTGGGCGTGC
  TACTCAAAATCCAAGTGTTCTTACATGCCGAGGCCAACGCTGCCCTTGCTACTCTAACCGCAAAGCCTGC
  TTAGATTGTATATGTCGTGGCTGCCAAAACTCCTATATGGCCAATGGGGAGAAGAAGCTGGAGGCATTTG
  CCGTGCCAGAAAAGGCCTTGGAGCAGACCAGGCTCACTTTGGGCATTAACGTGACTAGCATTGCTGTGCG
  TAACGCTAGTACCAGCACCAGTGTAATAAATGTCACAGGGTCCCCAGTAACGACGTTTTTAGCTGCCAGT
  ACACATGATGATAAAAGTTTGGATGAAGCTATAGACATGAGATTCGACTGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210829 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MNPVNATALYISASRLVLNYDPGDPKAFTEINRLLPYFRQSLSCCVCGHLLQDPIAPTNSTCQHYVCKTC
  KGKKMMMKPSCSWCKDYEQFEENKQLSILVNCYKKLCEYITQTTLARDIIEAVDCSSDILALLNDGSLFC
  EETEKPSDSSFTLCLTHSPLPSTSEPTTDPQASLSPMSESTLSIAIGSSVINGLPTYNGLSIDRFGINIP
  SPEHSNTIDVCNTVDIKTEDLSDSLPPVCDTVATDLCSTGIDICSFSEDIKPGDSLLLSVEEVLRSLETV
  SNTEVCCPNLQPNLEATVSNGPFLQLSSQSLSHNVFMSTSPALHGLSCTAATPKIAKLNRKRSRSESDSE
  KVQPLPISTIIRGPTLGASAPVTVKRESKISLQPIATVPNGGTTPKISKTVLLSTKSMKKSHEHGSKKSH
  SKTKPGILKKDKAVKEKIPSHHFMPGSPTKTVYKKPQEKKGCKCGRATQNPSVLTCRGQRCPCYSNRKAC
  LDCICRGCQNSYMANGEKKLEAFAVPEKALEQTRLTLGINVTSIAVRNASTSTSVINVTGSPVTTFLAAS
  THDDKSLDEAIDMRFDC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018133
• ORF Size: 1731 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018133.4
• RefSeq Size: 4696 bp
• RefSeq ORF: 1734 bp
• Locus ID: 55167
• UniProt ID: Q9HCI7
• Cytogenetics: 3q22.3
• Protein Families: Druggable Genome
• MW: 62.5 kDa
• Gene Summary: Component of histone acetyltransferase complex responsible for the majority of histone H4 acetylation at lysine 16 which is implicated in the formation of higher-order chromatin structure. Acts as an E3 ubiquitin ligase that promotes monoubiquitination of histone H2B at 'Lys-35' (H2BK34Ub), but not that of H2A. This activity is greatly enhanced by heterodimerization with MSL1. H2B ubiquitination in turn stimulates histine H3 methylation at 'Lys-4' (H3K4me) and 'Lys-79' (H3K79me) and leads to gene activation, including that of HOXA9 and MEIS1.[UniProtKB/Swiss-Prot Function]