galectin 9 (LGALS9) (NM_002308) Human Tagged ORF Clone
LGALS9 (Myc-DDK-tagged)-Human lectin, galactoside-binding, soluble, 9 (LGALS9), transcript variant 2
|Type||Human Tagged ORF Clone|
|E. coli Selection||Kanamycin (25 ug/mL)|
|Mammalian Cell Selection||Neomycin|
>RC210750 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)
>RC210750 protein sequence
Red=Cloning site Green=Tags(s)
Sequencher program is needed, download here.
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||969 bp|
|OTI Disclaimer||Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at email@example.com or by calling 301.340.3188 option 3 for pricing and delivery.
The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq Size||1739 bp|
|RefSeq ORF||972 bp|
|Gene Summary||The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. The protein encoded by this gene is an S-type lectin. It is overexpressed in Hodgkin's disease tissue and might participate in the interaction between the H&RS cells with their surrounding cells and might thus play a role in the pathogenesis of this disease and/or its associated immunodeficiency. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2008]|
|cDNA Clone Resources|
|RC210750L1||Lenti ORF clone of Human lectin, galactoside-binding, soluble, 9 (LGALS9), transcript variant 2, Myc-DDK-tagged||
|RC210750L2||Lenti ORF clone of Human lectin, galactoside-binding, soluble, 9 (LGALS9), transcript variant 2, mGFP tagged||
|RC210750L3||Lenti ORF clone of Human lectin, galactoside-binding, soluble, 9 (LGALS9), transcript variant 2, Myc-DDK-tagged||
|RC210750L4||Lenti ORF clone of Human lectin, galactoside-binding, soluble, 9 (LGALS9), transcript variant 2, mGFP tagged||
|RG210750||LGALS9 (GFP-tagged) - Human lectin, galactoside-binding, soluble, 9 (LGALS9), transcript variant 2||
|SC118707||LGALS9 (untagged)-Human lectin, galactoside-binding, soluble, 9 (LGALS9), transcript variant 2||