SPCS2 (NM_014752) Human Tagged ORF Clone

CAT#: RC210745

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SPCS2 (Myc-DDK-tagged)-Human signal peptidase complex subunit 2 homolog (S. cerevisiae) (SPCS2)



  "NM_014752" in other vectors (5)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 300.00

USD 450.00


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Size
    • 10 ug


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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol SPCS2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC210745 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCGGCAGCTGTACAGGGCGGGAGAAGCGGTGGTAGCGGAGGCTGTAGTGGGGCTGGTGGTGCTT
CCAACTGCGGGACAGGAAGTGGCCGTAGCGGCTTGTTGGATAAGTGGAAGATAGATGATAAGCCTGTAAA
AATTGACAAGTGGGATGGATCAGCTGTGAAAAACTCTTTGGATGATTCTGCCAAAAAGGTACTTCTGGAA
AAATACAAATATGTGGAGAATTTTGGTCTAATTGATGGTCGCCTCACCATCTGTACAATCTCCTGTTTCT
TTGCCATAGTGGCTTTGATTTGGGATTATATGCACCCCTTTCCAGAGTCCAAACCCGTTTTGGCTTTGTG
TGTCATATCCTATTTTGTGATGATGGGGATTCTGACCATTTATACCTCATATAAGGAGAAGAGCATCTTT
CTCGTGGCCCACAGGAAAGATCCTACAGGAATGGATCCTGATGATATTTGGCAGCTGTCCTCCAGTCTTA
AAAGGTTTGATGACAAATACACCTTGAAGCTGACCTTCATCAGTGGGAGAACAAAGCAGCAGCGGGAAGC
CGAGTTCACAAAGTCCATTGCTAAGTTTTTTGACCACAGTGGGACACTGGTCATGGATGCATATGAGCCT
GAAATATCCAGGCTCCATGACAGTCTTGCCATAGAAAGAAAAATAAAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC210745 protein sequence
Red=Cloning site Green=Tags(s)

MAAAAVQGGRSGGSGGCSGAGGASNCGTGSGRSGLLDKWKIDDKPVKIDKWDGSAVKNSLDDSAKKVLLE
KYKYVENFGLIDGRLTICTISCFFAIVALIWDYMHPFPESKPVLALCVISYFVMMGILTIYTSYKEKSIF
LVAHRKDPTGMDPDDIWQLSSSLKRFDDKYTLKLTFISGRTKQQREAEFTKSIAKFFDHSGTLVMDAYEP
EISRLHDSLAIERKIK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_014752
ORF Size 678 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_014752.3
RefSeq Size 2731 bp
RefSeq ORF 681 bp
Locus ID 9789
UniProt ID Q15005
Cytogenetics 11q13.4
Protein Families Protease, Transmembrane
MW 25 kDa
Gene Summary Component of the microsomal signal peptidase complex which removes signal peptides from nascent proteins as they are translocated into the lumen of the endoplasmic reticulum.[UniProtKB/Swiss-Prot Function]

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