RNF130 (NM_018434) Human Tagged ORF Clone

CAT#: RC210706

RNF130 (Myc-DDK-tagged)-Human ring finger protein 130 (RNF130)

Product Images

Circular map for RC210706

• 

  Western blot validation of overexpression lysate (Cat# LY413021) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC210706 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RNF130
• Synonyms: G1RP; G1RZFP; GOLIATH; GP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210706 representing NM_018434
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCTGCGCGGGGCGGGCGGGCCCTGCCCGGCTCGCCGCGCTCGCCCTGCTGACCTGCAGCCTGTGGC
  CGGCACGGGCAGACAACGCGAGCCAGGAGTACTACACAGCGCTCATCAACGTGACGGTGCAGGAGCCCGG
  CCGCGGCGCCCCGCTCACGTTTCGCATCGACCGCGGGCGCTACGGGCTTGACTCCCCCAAGGCCGAGGTC
  CGCGGCCAGGTGCTGGCGCCGCTGCCCCTCCACGGAGTTGCTGATCATCTGGGCTGTGATCCACAAACCC
  GGTTCTTTGTCCCTCCTAATATCAAACAGTGGATTGCCTTGCTGCAGAGGGGAAACTGCACGTTTAAAGA
  GAAAATATCACGGGCCGCTTTCCACAATGCAGTTGCTGTAGTCATCTACAATAATAAATCCAAAGAGGAG
  CCAGTTACCATGACTCATCCAGGCACTGGAGATATTATTGCTGTCATGATAACAGAATTGAGGGGTAAGG
  ATATTTTGAGTTATCTGGAGAAAAACATCTCTGTACAAATGACAATAGCTGTTGGAACTCGAATGCCACC
  GAAGAACTTCAGCCGTGGCTCTCTAGTCTTCGTGTCAATATCCTTTATTGTTTTGATGATTATTTCTTCA
  GCATGGCTCATATTCTACTTCATTCAGAAGATCAGGTACACAAATGCACGCGACAGGAACCAGCGTCGTC
  TCGGAGATGCAGCCAAGAAAGCCATCAGTAAATTGACAACCAGGACAGTAAAGAAGGGTGACAAGGAAAC
  TGACCCAGACTTTGATCATTGTGCAGTCTGCATAGAGAGCTATAAGCAGAATGATGTCGTCCGAATTCTC
  CCCTGCAAGCATGTTTTCCACAAATCCTGCGTGGATCCCTGGCTTAGTGAACATTGTACCTGTCCTATGT
  GCAAACTTAATATATTGAAGGCCCTGGGAATTGTGCCGAATTTGCCATGTACTGATAACGTAGCATTCGA
  TATGGAAAGGCTCACCAGAACCCAAGCTGTTAACCGAAGATCAGCCCTCGGCGACCTCGCCGGCGACAAC
  TCCCTTGGCCTTGAGCCACTTCGAACTTCGGGGATCTCACCTCTTCCTCAGGATGGGGAGCTCACTCCGA
  GAACAGGAGAAATCAACATTGCAGTAACAAAAGAATGGTTTATTATTGCCAGTTTTGGCCTCCTCAGTGC
  CCTCACACTCTGCTACATGATCATCAGAGCCACAGCTAGCTTGAATGCTAATGAGGTAGAATGGTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210706 representing NM_018434
  Red=Cloning site Green=Tags(s)
  
  MSCAGRAGPARLAALALLTCSLWPARADNASQEYYTALINVTVQEPGRGAPLTFRIDRGRYGLDSPKAEV
  RGQVLAPLPLHGVADHLGCDPQTRFFVPPNIKQWIALLQRGNCTFKEKISRAAFHNAVAVVIYNNKSKEE
  PVTMTHPGTGDIIAVMITELRGKDILSYLEKNISVQMTIAVGTRMPPKNFSRGSLVFVSISFIVLMIISS
  AWLIFYFIQKIRYTNARDRNQRRLGDAAKKAISKLTTRTVKKGDKETDPDFDHCAVCIESYKQNDVVRIL
  PCKHVFHKSCVDPWLSEHCTCPMCKLNILKALGIVPNLPCTDNVAFDMERLTRTQAVNRRSALGDLAGDN
  SLGLEPLRTSGISPLPQDGELTPRTGEINIAVTKEWFIIASFGLLSALTLCYMIIRATASLNANEVEWF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018434
• ORF Size: 1257 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018434.6
• RefSeq Size: 1865 bp
• RefSeq ORF: 1260 bp
• Locus ID: 55819
• UniProt ID: Q86XS8
• Cytogenetics: 5q35.3
• Domains: RING
• Protein Families: Druggable Genome, Transmembrane
• MW: 46.2 kDa
• Gene Summary: The protein encoded by this gene contains a RING finger motif and is similar to g1, a Drosophila zinc-finger protein that is expressed in mesoderm and involved in embryonic development. The expression of the mouse counterpart was found to be upregulated in myeloblastic cells following IL3 deprivation, suggesting that this gene may regulate growth factor withdrawal-induced apoptosis of myeloid precursor cells. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]