ZNF684 (NM_152373) Human Tagged ORF Clone

CAT#: RC210673

ZNF684 (Myc-DDK-tagged)-Human zinc finger protein 684 (ZNF684)

Product Images

Circular map for RC210673

• 

  Western blot validation of overexpression lysate (Cat# LY407620) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC210673 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZNF684
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210673 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATCAGCTTCCAGGAATCAGTGACATTCCAGGATGTGGCTGTGGATTTCACTGCAGAGGAGTGGCAGC
  TGCTTGATTGTGCTGAGAGAACCCTGTATTGGGATGTGATGTTGGAGAACTATAGAAACCTCATCTCAGT
  GGGATGTCCAATTACCAAAACAAAAGTGATCCTCAAGGTAGAGCAAGGACAAGAGCCATGGATGGTGGAG
  GGAGCGAATCCACACGAGAGCTCTCCAGAATCTGACTACCCACTTGTTGATGAACCAGGGAAGCATCGGG
  AAAGCAAAGACAATTTTTTGAAGTCAGTTTTGCTCACATTCAATAAAATTCTGACTATGGAGAGAATCCA
  CCATTATAATATGAGCACAAGTCTTAATCCAATGAGAAAAAAATCATATAAATCGTTTGAGAAGTGTTTG
  CCACCTAATTTAGACTTACTTAAATATAATAGAAGTTATACTGTAGAAAACGCTTATGAATGCAGTGAAT
  GCGGGAAAGCCTTCAAAAAGAAGTTTCATTTCATTAGACATGAAAAAAATCATACAAGGAAAAAACCTTT
  TGAATGCAATGACTGTGGAAAAGCCTATAGCAGGAAGGCACACCTTGCAACTCATCAGAAAATTCATAAT
  GGAGAGAGACCCTTTGTGTGCAATGATTGTGGGAAGGCGTTTATGCATAAAGCCCAACTCGTGGTCCACC
  AGAGACTTCACACTGGAGAGAAGCCTTATGAGTGCAGTCAATGTGGGAAAACATTCACTTGGAACTCCTC
  ATTTAATCAACACGTGAAATCTCATACACTTGAGAAGTCATTTGAATGTAAGGAATGTGGGAAAACCTTC
  AGGTATAGTTCATCCCTTTATAAACATTCCAGATTTCATACAGGAGAGAAACCCTACCAGTGTATCATAT
  GTGGCAAAGCTTTTGGCAACACATCCGTGCTTGTTACACACCAAAGAATTCATACAGGAGAGAAACCTTA
  CAGTTGTATTGAATGTGGCAAAGCCTTCATCAAGAAGTCCCATCTCCTCAGACATCAGATAACTCATACA
  GGAGAGAAGCCCTATGAATGTAACAGATGTGGGAAAGCATTTTCCCAGAAGTCAAATCTTATTGTACATC
  AGAAAATTCATACA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210673 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MISFQESVTFQDVAVDFTAEEWQLLDCAERTLYWDVMLENYRNLISVGCPITKTKVILKVEQGQEPWMVE
  GANPHESSPESDYPLVDEPGKHRESKDNFLKSVLLTFNKILTMERIHHYNMSTSLNPMRKKSYKSFEKCL
  PPNLDLLKYNRSYTVENAYECSECGKAFKKKFHFIRHEKNHTRKKPFECNDCGKAYSRKAHLATHQKIHN
  GERPFVCNDCGKAFMHKAQLVVHQRLHTGEKPYECSQCGKTFTWNSSFNQHVKSHTLEKSFECKECGKTF
  RYSSSLYKHSRFHTGEKPYQCIICGKAFGNTSVLVTHQRIHTGEKPYSCIECGKAFIKKSHLLRHQITHT
  GEKPYECNRCGKAFSQKSNLIVHQKIHT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_152373
• ORF Size: 1134 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_152373.4
• RefSeq Size: 2108 bp
• RefSeq ORF: 1137 bp
• Locus ID: 127396
• UniProt ID: Q5T5D7
• Cytogenetics: 1p34.2
• MW: 43.9 kDa
• Gene Summary: May be involved in transcriptional regulation.[UniProtKB/Swiss-Prot Function]