FIP1L1 (NM_030917) Human Tagged ORF Clone

CAT#: RC210617

FIP1L1 (Myc-DDK-tagged)-Human FIP1 like 1 (S. cerevisiae) (FIP1L1), transcript variant 1

Product Images

Circular map for RC210617

• 

  Western blot validation of overexpression lysate (Cat# LY410662) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC210617 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FIP1L1
• Synonyms: FIP1; hFip1; Rhe
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210617 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGGCCGGCGAGGTCGAGCGCCTAGTGTCGGAGCTGAGCGGCGGGACCGGAGGGGATGAGGAGGAAG
  AGTGGCTCTATGGCGGCCCATGGGACGTGCATGTGCACAGTGATTTGGCAAAGGACCTAGATGAAAATGA
  AGTTGAAAGGCCAGAAGAAGAAAATGCCAGTGCTAATCCTCCATCTGGAATTGAAGATGAAACTGCTGAA
  AATGGTGTACCAAAACCGAAAGTGACTGAGACCGAAGATGATAGTGATAGTGACAGCGATGATGATGAAG
  ATGATGTTCATGTCACTATAGGAGACATTAAAACGGGAGCACCACAGTATGGGAGTTATGGTACAGCACC
  TGTAAATCTTAACATCAAGACAGGGGGAAGAGTTTATGGAACTACAGGGACAAAAGTCAAAGGAGTAGAC
  CTTGATGCACCTGGAAGCATTAATGGAGTTCCACTCTTAGAGGTAGATTTGGATTCTTTTGAAGATAAAC
  CATGGCGTAAACCTGGTGCTGATCTTTCTGATTATTTTAATTATGGGTTTAATGAAGATACCTGGAAAGC
  TTACTGTGAAAAACAAAAGAGGATACGAATGGGACTTGAAGTTATACCAGTAACCTCTACTACAAATAAA
  ATTACGGCCGAAGACTGTACTATGGAAGTTACACCAGGTGCAGAGATCCAAGATGGCAGATTCAATCTTT
  TTAAGGTACAGCAGGGAAGAACTGGAAACTCAGAGAAAGAAACTGCCCTTCCATCTACAAAAGCTGAGTT
  TACTTCTCCTCCTTCTTTGTTCAAGACTGGGCTTCCACCGAGCAGAAACAGCACTTCTTCTCAGTCTCAG
  ACAAGTACTGCCTCCAGAAAAGCCAATTCAAGCGTTGGGAAGTGGCAGGATCGATATGGGAGGGCCGAAT
  CACCTGATCTAAGGAGATTACCTGGGGCAATTGATGTTATCGGTCAGACTATAACTATCAGCCGAGTAGA
  AGGCAGGCGACGGGCAAATGAGAACAGCAACATACAGGTCCTTTCTGAAAGATCTGCTACTGAAGTAGAC
  AACAATTTTAGCAAACCACCTCCGTTTTTCCCTCCAGGAGCTCCTCCCACTCACCTTCCACCTCCTCCAT
  TTCTTCCACCTCCTCCGACTGTCAGCACTGCTCCACCTCTGATTCCACCACCGGGTTTTCCTCCTCCACC
  AGGCGCTCCACCTCCATCTCTTATACCAACAATAGAAAGTGGACATTCCTCTGGTTATGATAGTCGTTCT
  GCACGTGCATTTCCATATGGCAATGTTGCCTTTCCCCATCTTCCTGGTTCTGCTCCTTCGTGGCCTAGTC
  TTGTGGACACCAGCAAGCAGTGGGACTATTATGCCAGAAGAGAGAAAGACCGAGATAGAGAGAGAGACAG
  AGACAGAGAGCGAGACCGTGATCGGGACAGAGAAAGAGAACGCACCAGAGAGAGAGAGAGGGAGCGTGAT
  CACAGTCCTACACCAAGTGTTTTCAACAGCGATGAAGAACGATACAGATACAGGGAATATGCAGAAAGAG
  GTTATGAGCGTCACAGAGCAAGTCGAGAAAAAGAAGAACGACATAGAGAAAGACGACACAGGGAGAAAGA
  GGAAACCAGACATAAGTCTTCTCGAAGTAATAGTAGACGTCGCCATGAAAGTGAAGAAGGAGATAGTCAC
  AGGAGACACAAACACAAAAAATCTAAAAGAAGCAAAGAAGGAAAAGAAGCGGGCAGTGAGCCTGCCCCTG
  AACAGGAGAGCACCGAAGCTACACCTGCAGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210617 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSAGEVERLVSELSGGTGGDEEEEWLYGGPWDVHVHSDLAKDLDENEVERPEEENASANPPSGIEDETAE
  NGVPKPKVTETEDDSDSDSDDDEDDVHVTIGDIKTGAPQYGSYGTAPVNLNIKTGGRVYGTTGTKVKGVD
  LDAPGSINGVPLLEVDLDSFEDKPWRKPGADLSDYFNYGFNEDTWKAYCEKQKRIRMGLEVIPVTSTTNK
  ITAEDCTMEVTPGAEIQDGRFNLFKVQQGRTGNSEKETALPSTKAEFTSPPSLFKTGLPPSRNSTSSQSQ
  TSTASRKANSSVGKWQDRYGRAESPDLRRLPGAIDVIGQTITISRVEGRRRANENSNIQVLSERSATEVD
  NNFSKPPPFFPPGAPPTHLPPPPFLPPPPTVSTAPPLIPPPGFPPPPGAPPPSLIPTIESGHSSGYDSRS
  ARAFPYGNVAFPHLPGSAPSWPSLVDTSKQWDYYARREKDRDRERDRDRERDRDRDRERERTRERERERD
  HSPTPSVFNSDEERYRYREYAERGYERHRASREKEERHRERRHREKEETRHKSSRSNSRRRHESEEGDSH
  RRHKHKKSKRSKEGKEAGSEPAPEQESTEATPAE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_030917
• ORF Size: 1782 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_030917.4
• RefSeq Size: 2458 bp
• RefSeq ORF: 1785 bp
• Locus ID: 81608
• UniProt ID: Q6UN15
• Cytogenetics: 4q12
• Domains: Fip1
• MW: 66.5 kDa
• Gene Summary: This gene encodes a subunit of the CPSF (cleavage and polyadenylation specificity factor) complex that polyadenylates the 3' end of mRNA precursors. This gene, the homolog of yeast Fip1 (factor interacting with PAP), binds to U-rich sequences of pre-mRNA and stimulates poly(A) polymerase activity. Its N-terminus contains a PAP-binding site and its C-terminus an RNA-binding domain. An interstitial chromosomal deletion on 4q12 creates an in-frame fusion of human genes FIP1L1 and PDGFRA (platelet-derived growth factor receptor, alpha). The FIP1L1-PDGFRA fusion gene encodes a constitutively activated tyrosine kinase that joins the first 233 amino acids of FIP1L1 to the last 523 amino acids of PDGFRA. This gene fusion and chromosomal deletion is the cause of some forms of idiopathic hypereosinophilic syndrome (HES). This syndrome, recently reclassified as chronic eosinophilic leukemia (CEL), is responsive to treatment with tyrosine kinase inhibitors. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]