MYO1E (NM_004998) Human Tagged ORF Clone

CAT#: RC210513

MYO1E (Myc-DDK-tagged)-Human myosin IE (MYO1E)

Product Images

Circular map for RC210513

• 

  Western blot validation of overexpression lysate (Cat# LY417600) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC210513 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MYO1E
• Synonyms: FSGS6; HuncM-IC; MYO1C
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210513 representing NM_004998
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAAGCAAAGGTGTCTACCAGTACCACTGGCAAAGCCACAATGTCAAGCACAGTGGTGTGGACGACA
  TGGTGCTACTGTCCAAGATCACAGAGAACTCCATCGTGGAGAATCTGAAGAAGAGATACATGGATGACTA
  CATTTTTACATATATAGGATCTGTATTAATCTCAGTCAACCCTTTCAAGCAGATGCCATATTTTGGGGAA
  AAGGAAATTGAAATGTACCAAGGAGCGGCACAGTATGAAAACCCACCACATATCTATGCCCTTGCAGATA
  ATATGTACAGAAACATGATCATTGACAGAGAGAACCAGTGCGTCATTATCAGTGGTGAAAGTGGTGCTGG
  AAAAACAGTGGCTGCCAAATATATCATGAGCTACATCTCCAGAGTGTCTGGAGGAGGGACCAAAGTCCAG
  CACGTGAAGGACATTATCCTGCAGTCCAACCCGCTGCTGGAGGCCTTCGGGAACGCCAAGACCGTCCGGA
  ACAACAACTCCAGCCGATTTGGAAAATACTTTGAAATCCAGTTCAGTCCAGGTGGGGAACCAGATGGTGG
  AAAGATCTCCAACTTCCTTCTGGAAAAATCTAGGGTGGTGATGAGGAACCCAGGAGAGCGGAGTTTTCAC
  ATATTTTACCAGCTCATCGAGGGCGCCTCTGCAGAGCAGAAACACAGCCTTGGCATCACCAGCATGGACT
  ATTATTACTACCTGAGCCTCTCGGGCTCATACAAGGTTGATGACATTGACGACAGGCGGGAGTTTCAGGA
  AACTCTGCACGCCATGAATGTGATTGGGATCTTTGCAGAAGAGCAAACGCTGGTGTTGCAGATAGTGGCG
  GGTATTCTCCACCTGGGAAACATCAGCTTCAAAGAAGTTGGCAACTACGCGGCTGTGGAGAGTGAAGAGT
  TTTTAGCTTTTCCTGCATATCTGCTAGGGATAAACCAGGACCGGTTGAAAGAAAAGCTAACAAGCCGGCA
  GATGGATAGCAAGTGGGGAGGCAAATCCGAATCCATCCACGTGACCCTCAACGTAGAGCAGGCCTGTTAC
  ACCCGGGATGCGCTCGCCAAGGCCCTGCACGCCCGGGTCTTTGATTTCTTGGTAGATTCCATCAATAAAG
  CCATGGAGAAAGACCATGAAGAATACAACATTGGCGTCCTAGACATCTATGGCTTTGAAATATTCCAGAA
  AAATGGCTTTGAACAGTTTTGTATCAATTTTGTTAATGAAAAACTGCAGCAGATTTTTATTGAACTGACA
  TTAAAGGCAGAACAGGAAGAATATGTTCAAGAGGGAATAAGATGGACACCCATTGAGTACTTTAATAATA
  AAATCGTATGTGACCTCATAGAGAACAAAGTGAACCCTCCTGGCATCATGAGCATCCTGGATGACGTGTG
  CGCCACGATGCATGCGGTGGGTGAGGGGGCAGATCAGACGCTGCTCCAGAAACTTCAGATGCAGATTGGG
  AGTCATGAGCACTTCAACAGTTGGAACCAAGGCTTCATCATTCATCATTATGCTGGGAAGGTATCCTATG
  ACATGGATGGCTTTTGTGAAAGGAACCGGGATGTGCTTTTTATGGATCTCATCGAGCTTATGCAGAGCAG
  CGAGCTGCCTTTCATAAAGTCTTTATTTCCGGAAAATCTGCAGGCTGACAAGAAAGGGCGCCCAACTACT
  GCCGGAAGCAAAATAAAGAAACAAGCCAATGACCTTGTGAGCACCCTGATGAAATGTACGCCCCACTACA
  TTCGCTGCATCAAGCCAAACGAAACCAAGAAGCCCAGAGACTGGGAGGAAAGCAGGGTAAAGCATCAAGT
  CGAATATTTGGGTCTGAAAGAGAACATTCGAGTGAGAAGAGCTGGCTATGCCTATCGGCGCATCTTCCAA
  AAATTCCTACAGAGGTATGCCATTCTGACCAAAGCCACCTGGCCTTCTTGGCAGGGAGAGGAGAAGCAAG
  GCGTCCTGCACCTGCTGCAGTCGGTCAACATGGACAGCGACCAGTTCCAGCTGGGGAGGAGTAAAGTGTT
  CATCAAAGCCCCCGAGTCTCTATTTCTTTTAGAAGAGATGAGAGAGAGAAAGTATGATGGGTATGCTCGA
  GTGATACAGAAATCATGGAGGAAATTCGTGGCCCGGAAGAAATACGTTCAAATGAGAGAAGAAGCCTCAG
  ACCTCTTATTGAACAAGAAGGAGAGAAGGAGAAACAGTATTAACAGGAACTTTATAGGGGATTATATTGG
  GATGGAAGAGCACCCAGAACTCCAGCAGTTCGTGGGCAAGAGGGAGAAGATTGATTTCGCAGACACAGTC
  ACCAAGTATGACAGGAGGTTCAAGGGTGTAAAGCGAGACCTGCTCCTTACCCCAAAGTGCTTGTACTTAA
  TCGGACGAGAAAAAGTCAAACAGGGCCCAGACAAGGGCCTGGTGAAAGAAGTCCTGAAGCGGAAAATCGA
  GATAGAACGGATCTTGTCTGTGTCCCTCAGTACTATGCAGGATGACATTTTTATTCTCCATGAGCAAGAG
  TATGACAGTTTGCTTGAATCTGTCTTCAAAACTGAATTCCTAAGCCTCTTAGCAAAGCGTTACGAGGAGA
  AGACCCAGAAGCAACTACCTCTGAAATTCAGCAATACGCTTGAACTGAAGTTGAAAAAGGAAAACTGGGG
  CCCCTGGAGTGCAGGGGGCTCCCGGCAAGTGCAGTTCCACCAAGGGTTTGGGGACCTGGCTGTCCTCAAG
  CCCAGTAACAAAGTGCTGCAGGTCAGCATCGGACCTGGACTGCCCAAGAACTCCCGTCCTACCAGAAGGA
  ACACTACCCAAAATACAGGTTATTCCAGTGGGACTCAAAATGCCAACTACCCAGTGAGAGCTGCCCCTCC
  TCCCCCAGGATACCATCAGAACGGAGTCATCAGAAACCAGTATGTGCCATATCCCCATGCTCCTGGAAGC
  CAGAGGTCCAATCAGAAAAGCCTGTACACCTCCATGGCCCGCCCGCCCTTGCCTCGGCAGCAGTCTACCA
  GTTCAGACCGAGTGTCACAGACGCCAGAGAGCCTGGATTTCCTCAAGGTCCCGGACCAGGGAGCTGCAGG
  GGTCAGGAGACAAACAACCAGTCGGCCTCCCCCAGCAGGGGGCAGACCCAAGCCCCAGCCCAAGCCCAAG
  CCTCAGGTGCCACAGTGCAAGGCTTTGTATGCCTATGACGCTCAGGACACAGACGAACTCAGCTTTAATG
  CCAATGACATTATTGATATTATCAAAGAAGATCCTTCTGGCTGGTGGACGGGTCGACTACGAGGCAAGCA
  GGGCCTGTTCCCCAACAACTATGTGACCAAGATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210513 representing NM_004998
  Red=Cloning site Green=Tags(s)
  
  MGSKGVYQYHWQSHNVKHSGVDDMVLLSKITENSIVENLKKRYMDDYIFTYIGSVLISVNPFKQMPYFGE
  KEIEMYQGAAQYENPPHIYALADNMYRNMIIDRENQCVIISGESGAGKTVAAKYIMSYISRVSGGGTKVQ
  HVKDIILQSNPLLEAFGNAKTVRNNNSSRFGKYFEIQFSPGGEPDGGKISNFLLEKSRVVMRNPGERSFH
  IFYQLIEGASAEQKHSLGITSMDYYYYLSLSGSYKVDDIDDRREFQETLHAMNVIGIFAEEQTLVLQIVA
  GILHLGNISFKEVGNYAAVESEEFLAFPAYLLGINQDRLKEKLTSRQMDSKWGGKSESIHVTLNVEQACY
  TRDALAKALHARVFDFLVDSINKAMEKDHEEYNIGVLDIYGFEIFQKNGFEQFCINFVNEKLQQIFIELT
  LKAEQEEYVQEGIRWTPIEYFNNKIVCDLIENKVNPPGIMSILDDVCATMHAVGEGADQTLLQKLQMQIG
  SHEHFNSWNQGFIIHHYAGKVSYDMDGFCERNRDVLFMDLIELMQSSELPFIKSLFPENLQADKKGRPTT
  AGSKIKKQANDLVSTLMKCTPHYIRCIKPNETKKPRDWEESRVKHQVEYLGLKENIRVRRAGYAYRRIFQ
  KFLQRYAILTKATWPSWQGEEKQGVLHLLQSVNMDSDQFQLGRSKVFIKAPESLFLLEEMRERKYDGYAR
  VIQKSWRKFVARKKYVQMREEASDLLLNKKERRRNSINRNFIGDYIGMEEHPELQQFVGKREKIDFADTV
  TKYDRRFKGVKRDLLLTPKCLYLIGREKVKQGPDKGLVKEVLKRKIEIERILSVSLSTMQDDIFILHEQE
  YDSLLESVFKTEFLSLLAKRYEEKTQKQLPLKFSNTLELKLKKENWGPWSAGGSRQVQFHQGFGDLAVLK
  PSNKVLQVSIGPGLPKNSRPTRRNTTQNTGYSSGTQNANYPVRAAPPPPGYHQNGVIRNQYVPYPHAPGS
  QRSNQKSLYTSMARPPLPRQQSTSSDRVSQTPESLDFLKVPDQGAAGVRRQTTSRPPPAGGRPKPQPKPK
  PQVPQCKALYAYDAQDTDELSFNANDIIDIIKEDPSGWWTGRLRGKQGLFPNNYVTKI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004998
• ORF Size: 3324 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004998.4
• RefSeq Size: 4729 bp
• RefSeq ORF: 3327 bp
• Locus ID: 4643
• UniProt ID: Q12965
• Cytogenetics: 15q22.2
• Domains: IQ, SH3, myosin_head
• MW: 126.9 kDa
• Gene Summary: This gene encodes a member of the nonmuscle class I myosins which are a subgroup of the unconventional myosin protein family. The unconventional myosin proteins function as actin-based molecular motors. Class I myosins are characterized by a head (motor) domain, a regulatory domain and a either a short or long tail domain. Among the class I myosins, this protein is distinguished by a long tail domain that is involved in crosslinking actin filaments. This protein localizes to the cytoplasm and may be involved in intracellular movement and membrane trafficking. Mutations in this gene are the cause of focal segmental glomerulosclerosis-6. This gene has been referred to as myosin IC in the literature but is distinct from the myosin IC gene located on chromosome 17. [provided by RefSeq, Jan 2012]