KCC4 (SLC12A7) (NM_006598) Human Tagged ORF Clone

CAT#: RC210512

SLC12A7 (Myc-DDK-tagged)-Human solute carrier family 12 (potassium/chloride transporters), member 7 (SLC12A7)

Product Images

Circular map for RC210512

• 

  Western blot validation of overexpression lysate (Cat# LY416525) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC210512 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: KCC4
• Synonyms: KCC4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210512 representing NM_006598
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGC
  GCCC
  
  ATGCCCACCAACTTCACCGTGGTGCCCGTGGAGGCTCACGCCGACGGCGGCGGGGACGAGACTGCCGAGC
  GGACGGAGGCTCCGGGCACCCCCGAGGGCCCCGAGCCCGAGCGCCCCAGCCCGGGAGATGGAAATCCAAG
  AGAAAACAGCCCATTCCTCAACAATGTCGAGGTGGAACAAGAGAGCTTCTTTGAAGGGAAGAACATGGCA
  CTTTTCGAGGAGGAGATGGACAGTAACCCCATGGTGTCCTCGCTGCTCAACAAGCTGGCCAACTACACCA
  ACCTGAGCCAGGGCGTGGTGGAGCACGAGGAGGACGAGGAGAGCCGGCGGCGGGAGGCCAAGGCTCCGCG
  CATGGGCACCTTCATCGGCGTCTACCTGCCGTGCCTGCAGAACATCCTGGGCGTCATCCTCTTCCTGCGC
  CTGACGTGGATCGTGGGGGTGGCTGGTGTCCTGGAGTCCTTCCTCATCGTGGCCATGTGCTGCACATGTA
  CAATGCTGACCGCCATTTCCATGAGTGCGATCGCTACCAACGGTGTGGTCCCAGCTGGCGGGTCCTACTA
  CATGATATCGCGCTCGCTGGGACCCGAGTTTGGAGGCGCTGTCGGCCTCTGCTTCTACCTGGGCACGACG
  TTTGCAGGGGCCATGTATATTTTGGGGACCATCGAGATTTTTCTGACGTACATCTCCCCGGGTGCGGCCA
  TCTTCCAGGCGGAGGCTGCAGGTGGCGAGGCGGCCGCCATGCTGCACAACATGCGTGTGTACGGCACGTG
  CACGCTCGTGCTCATGGCCCTGGTGGTCTTCGTGGGCGTCAAGTATGTCAACAAGCTGGCGCTGGTCTTC
  CTGGCCTGCGTCGTGCTGTCCATCCTGGCCATCTATGCCGGCGTCATCAAGTCTGCCTTCGACCCCCCGG
  ACATCCCGGTCTGCCTCCTGGGGAACCGCACGCTGTCACGGCGCAGCTTCGATGCCTGCGTCAAGGCCTA
  CGGCATCCACAACAACTCAGCCACCTCCGCGCTCTGGGGCCTCTTCTGCAACGGCTCCCAGCCCAGCGCC
  GCCTGTGACGAGTACTTCATCCAGAACAACGTCACCGAAATCCAGGGCATCCCGGGCGCGGCCAGTGGTG
  TCTTCCTGGAGAACCTGTGGAGTACGTACGCGCACGCGGGGGCGTTTGTGGAGAAGAAAGGTGTGCCCTC
  GGTGCCCGTGGCAGAGGAGAGCCGTGCCAGCGCACTGCCCTACGTGCTCACCGACATCGCGGCCTCCTTC
  ACCCTGCTGGTTGGCATCTACTTCCCCTCCGTGACCGGTATCATGGCGGGTTCAAACCGGTCCGGGGACC
  TCAAGGATGCACAGAAGTCCATCCCCACGGGGACCATCCTGGCCATAGTGACGACGTCTTTCATCTATCT
  CTCCTGCATTGTGCTGTTTGGGGCCTGCATTGAAGGCGTGGTCTTACGAGATAAGTTCGGGGAGGCCCTG
  CAGGGGAACCTGGTCATCGGCATGCTGGCCTGGCCCTCCCCCTGGGTCATCGTCATCGGCTCCTTCTTCT
  CCACCTGCGGTGCCGGCCTGCAGAGCCTCACGGGGGCACCGCGCCTACTGCAGGCCATTGCCCGTGACGG
  CATCGTCCCCTTCCTGCAGGTGTTTGGCCACGGGAAGGCCAACGGGGAGCCCACGTGGGCGCTGCTGCTG
  ACAGTCCTCATCTGCGAGACTGGCATCCTCATCGCCTCTCTGGACAGCGTGGCCCCGATCCTCTCCATGT
  TCTTCCTCATGTGCTACCTGTTCGTGAACCTGGCCTGCGCCGTGCAGACCCTGCTACGTACCCCCAACTG
  GCGTCCACGCTTCAAGTTCTACCACTGGACCCTGTCCTTTCTGGGTATGAGCCTGTGCCTGGCGCTGATG
  TTCATCTGCTCCTGGTACTACGCGCTGTCCGCCATGCTCATCGCTGGCTGCATCTACAAGTACATCGAGT
  ACCGCGGGGCCGAGAAGGAGTGGGGCGATGGCATCCGTGGCCTATCCCTGAACGCCGCCCGCTACGCCCT
  GCTGCGCGTGGAGCACGGTCCCCCCCACACCAAGAACTGGAGGCCCCAGGTGCTGGTGATGCTGAACCTG
  GACGCGGAGCAGGCCGTGAAGCACCCCCGCCTGCTGTCCTTCACGTCGCAGCTGAAGGCCGGCAAGGGCC
  TGACCATCGTGGGCTCGGTGCTGGAGGGGACGTACCTGGACAAGCACATGGAGGCTCAGCGGGCCGAGGA
  GAACATACGGTCCCTAATGAGCACAGAGAAGACCAAGGGCTTCTGCCAGCTGGTGGTCTCGTCCAGCCTG
  CGGGATGGCATGTCCCACCTGATCCAGTCGGCCGGCCTGGGCGGCCTGAAGCACAACACGGTGCTCATGG
  CCTGGCCCGCATCCTGGAAGCAGGAGGACAACCCCTTCTCCTGGAAGAACTTTGTGGACACCGTCCGCGA
  CACCACCGCCGCGCACCAGGCTCTGCTGGTGGCCAAGAACGTCGACTCGTTTCCGCAAAACCAGGAGCGC
  TTCGGCGGGGGCCACATCGACGTGTGGTGGATCGTGCACGACGGCGGCATGCTCATGCTGCTGCCCTTCC
  TGCTGCGCCAGCACAAGGTGTGGAGGAAGTGCCGGATGCGTATCTTCACCGTGGCCCAGGTGGACGACAA
  CAGCATCCAGATGAAGAAGGACCTGCAGATGTTCTTGTATCACTTGCGCATCAGCGCCGAGGTGGAGGTG
  GTGGAGATGGTTGAAAACGACATATCTGCTTTCACCTACGAGAGGACACTAATGATGGAGCAGAGGTCGC
  AGATGCTGAAGCAGATGCAGCTGTCCAAGAACGAGCAGGAGCGAGAGGCCCAGCTGATCCACGACAGGAA
  CACCGCGTCCCACACCGCGGCGGCAGCCAGGACCCAAGCGCCGCCTACGCCAGACAAGGTGCAGATGACC
  TGGACCAGGGAGAAGCTGATCGCTGAGAAGTACAGGAGCAGAGACACCAGCCTATCCGGTTTCAAAGACC
  TCTTCAGCATGAAGCCGGACCAGTCCAACGTCAGGCGGATGCACACGGCTGTGAAGCTCAATGGCGTCGT
  CCTCAACAAGTCCCAGGATGCGCAGCTGGTCCTGCTCAACATGCCAGGTCCTCCCAAAAACCGGCAGGGA
  GACGAGAACTACATGGAGTTTCTTGAAGTCCTGACCGAGGGGCTGAACAGAGTCCTCCTGGTCAGGGGTG
  GCGGCCGGGAGGTGATCACCATCTACTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210512 representing NM_006598
  Red=Cloning site Green=Tags(s)
  
  MPTNFTVVPVEAHADGGGDETAERTEAPGTPEGPEPERPSPGDGNPRENSPFLNNVEVEQESFFEGKNMA
  LFEEEMDSNPMVSSLLNKLANYTNLSQGVVEHEEDEESRRREAKAPRMGTFIGVYLPCLQNILGVILFLR
  LTWIVGVAGVLESFLIVAMCCTCTMLTAISMSAIATNGVVPAGGSYYMISRSLGPEFGGAVGLCFYLGTT
  FAGAMYILGTIEIFLTYISPGAAIFQAEAAGGEAAAMLHNMRVYGTCTLVLMALVVFVGVKYVNKLALVF
  LACVVLSILAIYAGVIKSAFDPPDIPVCLLGNRTLSRRSFDACVKAYGIHNNSATSALWGLFCNGSQPSA
  ACDEYFIQNNVTEIQGIPGAASGVFLENLWSTYAHAGAFVEKKGVPSVPVAEESRASALPYVLTDIAASF
  TLLVGIYFPSVTGIMAGSNRSGDLKDAQKSIPTGTILAIVTTSFIYLSCIVLFGACIEGVVLRDKFGEAL
  QGNLVIGMLAWPSPWVIVIGSFFSTCGAGLQSLTGAPRLLQAIARDGIVPFLQVFGHGKANGEPTWALLL
  TVLICETGILIASLDSVAPILSMFFLMCYLFVNLACAVQTLLRTPNWRPRFKFYHWTLSFLGMSLCLALM
  FICSWYYALSAMLIAGCIYKYIEYRGAEKEWGDGIRGLSLNAARYALLRVEHGPPHTKNWRPQVLVMLNL
  DAEQAVKHPRLLSFTSQLKAGKGLTIVGSVLEGTYLDKHMEAQRAEENIRSLMSTEKTKGFCQLVVSSSL
  RDGMSHLIQSAGLGGLKHNTVLMAWPASWKQEDNPFSWKNFVDTVRDTTAAHQALLVAKNVDSFPQNQER
  FGGGHIDVWWIVHDGGMLMLLPFLLRQHKVWRKCRMRIFTVAQVDDNSIQMKKDLQMFLYHLRISAEVEV
  VEMVENDISAFTYERTLMMEQRSQMLKQMQLSKNEQEREAQLIHDRNTASHTAAAARTQAPPTPDKVQMT
  WTREKLIAEKYRSRDTSLSGFKDLFSMKPDQSNVRRMHTAVKLNGVVLNKSQDAQLVLLNMPGPPKNRQG
  DENYMEFLEVLTEGLNRVLLVRGGGREVITIYS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: AscI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006598
• ORF Size: 3249 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006598.3
• RefSeq Size: 5239 bp
• RefSeq ORF: 3252 bp
• Locus ID: 10723
• UniProt ID: Q9Y666
• Cytogenetics: 5p15.33
• Protein Families: Transmembrane
• MW: 118.9 kDa
• Gene Summary: Mediates electroneutral potassium-chloride cotransport when activated by cell swelling. May mediate K(+) uptake into Deiters' cells in the cochlea and contribute to K(+) recycling in the inner ear. Important for the survival of cochlear outer and inner hair cells and the maintenance of the organ of Corti. May be required for basolateral Cl(-) extrusion in the kidney and contribute to renal acidification (By similarity).[UniProtKB/Swiss-Prot Function]