NUDT15 (NM_018283) Human Tagged ORF Clone

CAT#: RC210477

NUDT15 (Myc-DDK-tagged)-Human nudix (nucleoside diphosphate linked moiety X)-type motif 15 (NUDT15)

Product Images

Circular map for RC210477

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY NUDT15 (Cat# RC210477, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NUDT15 (Cat# TA811515)(1:2000). Positive lysates LY402670 (100ug) and LC402670 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY402670) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC210477 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified NUDT15 protein (Cat# TP310477). The protein was produced from HEK293T cells transfected with NUDT15 cDNA clone (Cat# RC210477) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NUDT15
• Synonyms: MTH2; NUDT15D
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210477 representing NM_018283
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACGGCCAGCGCACAGCCGCGCGGGCGGCGGCCAGGAGTCGGAGTCGGAGTCGTGGTGACCAGCTGCA
  AGCATCCGCGTTGCGTCCTCCTGGGGAAGAGGAAAGGCTCGGTTGGAGCTGGCAGTTTCCAACTCCCTGG
  AGGTCATCTGGAGTTCGGTGAAACCTGGGAAGAATGTGCTCAAAGGGAAACCTGGGAAGAAGCAGCTCTT
  CACCTGAAAAATGTTCACTTTGCCTCAGTTGTGAATTCTTTCATTGAGAAGGAGAATTACCATTATGTTA
  CTATATTAATGAAAGGAGAAGTGGATGTGACTCATGATTCAGAACCAAAGAATGTAGAGCCTGAAAAAAA
  TGAAAGTTGGGAGTGGGTTCCTTGGGAAGAACTGCCTCCCCTGGACCAGCTTTTCTGGGGACTGCGTTGT
  TTAAAAGAACAAGGCTATGATCCATTTAAAGAAGATCTGAACCATCTGGTGGGATACAAAGGAAATCATC
  TC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210477 representing NM_018283
  Red=Cloning site Green=Tags(s)
  
  MTASAQPRGRRPGVGVGVVVTSCKHPRCVLLGKRKGSVGAGSFQLPGGHLEFGETWEECAQRETWEEAAL
  HLKNVHFASVVNSFIEKENYHYVTILMKGEVDVTHDSEPKNVEPEKNESWEWVPWEELPPLDQLFWGLRC
  LKEQGYDPFKEDLNHLVGYKGNHL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018283
• ORF Size: 492 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018283.4
• RefSeq Size: 2022 bp
• RefSeq ORF: 495 bp
• Locus ID: 55270
• UniProt ID: Q9NV35
• Cytogenetics: 13q14.2
• Domains: NUDIX
• MW: 18.4 kDa
• Gene Summary: This gene encodes an enzyme that belongs to the Nudix hydrolase superfamily. Members of this superfamily catalyze the hydrolysis of nucleoside diphosphates, including substrates like 8-oxo-dGTP, which are a result of oxidative damage, and can induce base mispairing during DNA replication, causing transversions. The encoded enzyme is a negative regulator of thiopurine activation and toxicity. Mutations in this gene result in poor metabolism of thiopurines, and are associated with thiopurine-induced early leukopenia. Multiple pseudogenes of this gene have been identified. [provided by RefSeq, Apr 2016]