AVPR1A (NM_000706) Human Tagged ORF Clone

CAT#: RC210436

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AVPR1A (Myc-DDK-tagged)-Human arginine vasopressin receptor 1A (AVPR1A)



  "NM_000706" in other vectors (4)

USD 420.00


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Size
    • 10 ug


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Specifications

Product Data
Product Name AVPR1A (NM_000706) Human Tagged ORF Clone
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol AVPR1A
Synonyms AVPR1; AVPR V1a; V1aR
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Cell Selection Neomycin
Sequence Data
>RC210436 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCGTCTCTCCGCCGGTCCCGACGCGGGGCCCTCGGGCAACTCCAGCCCATGGTGGCCTCTGGCCACCG
GCGCTGGCAACACAAGCCGGGAGGCCGAAGCCCTCGGGGAGGGCAACGGCCCACCGAGGGACGTGCGCAA
CGAGGAGCTGGCCAAACTGGAGATCGCCGTGCTGGCGGTGACTTTCGCGGTGGCCGTGCTGGGCAACAGC
AGCGTACTGCTGGCTCTGCACCGGACGCCGCGCAAGACGTCCCGCATGCACCTCTTCATCCGACACCTCA
GCCTGGCCGACCTGGCCGTGGCATTCTTCCAGGTGCTGCCGCAAATGTGCTGGGACATCACCTACCGCTT
CCGCGGCCCCGACTGGCTGTGCCGCGTGGTGAAGCACCTGCAGGTGTTCGGCATGTTTGCGTCGGCCTAC
ATGCTGGTAGTCATGACAGCCGACCGCTACATCGCGGTGTGCCACCCGCTCAAGACTCTGCAACAGCCCG
CGCGCCGCTCGCGCCTCATGATCGCGGCCGCCTGGGTGCTGAGCTTCGTGCTGAGCACGCCGCAGTACTT
CGTCTTCTCCATGATCGAGGTGAACAATGTCACCAAGGCCCGCGACTGCTGGGCCACCTTCATCCAGCCC
TGGGGTTCTCGTGCCTACGTGACCTGGATGACGGGCGGCATCTTTGTGGCGCCCGTGGTCATCTTGGGTA
CCTGCTACGGCTTCATCTGCTACAACATCTGGTGCAACGTCCGCGGGAAGACGGCGTCGCGCCAGAGCAA
GGGTGCAGAGCAAGCGGGTGTGGCCTTCCAAAAGGGGTTCCTGCTCGCACCCTGTGTCAGCAGCGTGAAG
TCCATTTCCCGGGCCAAGATCCGCACGGTGAAGATGACTTTTGTGATCGTGACGGCTTACATCGTCTGCT
GGGCGCCTTTCTTCATCATCCAGATGTGGTCTGTCTGGGATCCCATGTCCGTCTGGACCGAATCGGAAAA
CCCTACCATCACCATCACTGCATTACTGGGTTCCTTGAATAGCTGCTGTAATCCCTGGATATACATGTTT
TTTAGTGGCCATCTCCTTCAAGACTGTGTTCAAAGCTTCCCATGCTGCCAAAACATGAAGGAAAAATTCA
ACAAAGAAGATACTGACAGTATGAGCAGAAGACAGACTTTTTATTCTAACAATCGAAGCCCAACAAACAG
TACGGGTATGTGGAAGGACTCGCCTAAATCTTCCAAGTCCATCAAATTCATTCCTGTTTCAACT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC210436 protein sequence
Red=Cloning site Green=Tags(s)

MRLSAGPDAGPSGNSSPWWPLATGAGNTSREAEALGEGNGPPRDVRNEELAKLEIAVLAVTFAVAVLGNS
SVLLALHRTPRKTSRMHLFIRHLSLADLAVAFFQVLPQMCWDITYRFRGPDWLCRVVKHLQVFGMFASAY
MLVVMTADRYIAVCHPLKTLQQPARRSRLMIAAAWVLSFVLSTPQYFVFSMIEVNNVTKARDCWATFIQP
WGSRAYVTWMTGGIFVAPVVILGTCYGFICYNIWCNVRGKTASRQSKGAEQAGVAFQKGFLLAPCVSSVK
SISRAKIRTVKMTFVIVTAYIVCWAPFFIIQMWSVWDPMSVWTESENPTITITALLGSLNSCCNPWIYMF
FSGHLLQDCVQSFPCCQNMKEKFNKEDTDSMSRRQTFYSNNRSPTNSTGMWKDSPKSSKSIKFIPVST

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
Tag Myc-DDK
ACCN NM_000706
ORF Size 1254 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_000706.1, NM_000706.2, NM_000706.3, NM_000706.4, NP_000697.1
RefSeq Size 7844 bp
RefSeq ORF 1257 bp
Locus ID 552
Cytogenetics 12q14.2
Protein Families Druggable Genome, GPCR, Transmembrane
Protein Pathways Calcium signaling pathway, Neuroactive ligand-receptor interaction, Vascular smooth muscle contraction
MW 46.8 kDa
Gene Summary The protein encoded by this gene acts as receptor for arginine vasopressin. This receptor belongs to the subfamily of G-protein coupled receptors which includes AVPR1B, V2R and OXT receptors. Its activity is mediated by G proteins which stimulate a phosphatidylinositol-calcium second messenger system. The receptor mediates cell contraction and proliferation, platelet aggregation, release of coagulation factor and glycogenolysis. [provided by RefSeq, Jul 2008]

Citations (3)

Other Versions

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