gamma C Crystallin (CRYGC) (NM_020989) Human Tagged ORF Clone
CAT#: RC210386
CRYGC (Myc-DDK-tagged)-Human crystallin, gamma C (CRYGC)
ORF Plasmid: tGFP
Lentiviral Particles: DDK DDK w/ Puro mGFP mGFP w/ Puro
AAV Particle: DDK
"NM_020989" in other vectors (6)
USD 198.00
USD 447.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | gamma C Crystallin |
Synonyms | CCL; CRYG3; CTRCT2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC210386 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGAAGATCACCTTCTATGAGGACAGGGCCTTCCAGGGCCGCAGCTACGAAACCACCACTGACTGCC CCAACCTGCAGCCGTATTTCAGCCGCTGCAACTCCATCCGGGTGGAGAGCGGCTGCTGGATGCTCTATGA GCGTCCCAACTACCAAGGTCAACAATACTTGCTGCGGCGAGGGGAGTACCCCGACTACCAGCAATGGATG GGCCTCAGCGACTCCATCCGCTCCTGTTGTCTCATCCCCCAAACAGTCTCCCACAGGCTGCGGCTGTACG AGAGGGAAGACCACAAAGGCCTCATGATGGAGCTGAGTGAAGACTGCCCCAGCATCCAGGACCGCTTCCA CCTCAGCGAGATCCGTTCCCTCCACGTGCTGGAGGGCTGCTGGGTCCTCTACGAGCTGCCCAACTACCGG GGGCGGCAATACCTGCTGAGGCCCCAAGAGTACAGGCGGTGCCAGGACTGGGGGGCCATGGATGCTAAGG CAGGCTCTTTGCGGAGAGTGGTGGATTTGTAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC210386 protein sequence
Red=Cloning site Green=Tags(s) MGKITFYEDRAFQGRSYETTTDCPNLQPYFSRCNSIRVESGCWMLYERPNYQGQQYLLRRGEYPDYQQWM GLSDSIRSCCLIPQTVSHRLRLYEREDHKGLMMELSEDCPSIQDRFHLSEIRSLHVLEGCWVLYELPNYR GRQYLLRPQEYRRCQDWGAMDAKAGSLRRVVDLY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_020989 |
ORF Size | 522 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_020989.4 |
RefSeq Size | 645 bp |
RefSeq ORF | 525 bp |
Locus ID | 1420 |
UniProt ID | P07315 |
Cytogenetics | 2q33.3 |
Protein Families | Druggable Genome |
MW | 20.9 kDa |
Gene Summary | This gene encodes a member of the beta/gamma-crystallin family of proteins. Crystallins constitute the major proteins of vertebrate eye lens and maintain the transparency and refractive index of the lens. This gene and several family members are present in a gene cluster on chromosome 2. Mutations in this gene have been shown to cause multiple types of cataract, including Coppock-like cataract and zonular pulverulent cataract, among others. [provided by RefSeq, Jan 2015] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC210386L1 | Lenti ORF clone of Human crystallin, gamma C (CRYGC), Myc-DDK-tagged |
USD 600.00 |
|
RC210386L2 | Lenti ORF clone of Human crystallin, gamma C (CRYGC), mGFP tagged |
USD 600.00 |
|
RC210386L3 | Lenti ORF clone of Human crystallin, gamma C (CRYGC), Myc-DDK-tagged |
USD 600.00 |
|
RC210386L4 | Lenti ORF clone of Human crystallin, gamma C (CRYGC), mGFP tagged |
USD 600.00 |
|
RG210386 | CRYGC (tGFP-tagged) - Human crystallin, gamma C (CRYGC) |
USD 500.00 |
|
SC304887 | CRYGC (untagged)-Human crystallin, gamma C (CRYGC) |
USD 330.00 |
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