CCN2 (NM_001901) Human Tagged ORF Clone
CTGF (Myc-DDK-tagged)-Human connective tissue growth factor (CTGF)
|Product Name||CCN2 (NM_001901) Human Tagged ORF Clone|
|Type||Human Tagged ORF Clone|
|Synonyms||CTGF; HCS24; IGFBP8; NOV2|
|E. coli Selection||Kanamycin (25 ug/mL)|
>RC210336 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)
>RC210336 protein sequence
Red=Cloning site Green=Tags(s)
Sequencher program is needed, download here.
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||1047 bp|
|OTI Disclaimer||Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at email@example.com or by calling 301.340.3188 option 3 for pricing and delivery.
The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq||NM_001901.1, NM_001901.2, NP_001892.1|
|Domains||IB, tsp_1, VWC, CT, Cys_knot|
|Protein Families||Druggable Genome, Secreted Protein|
|Gene Summary||The protein encoded by this gene is a mitogen that is secreted by vascular endothelial cells. The encoded protein plays a role in chondrocyte proliferation and differentiation, cell adhesion in many cell types, and is related to platelet-derived growth factor. Certain polymorphisms in this gene have been linked with a higher incidence of systemic sclerosis. [provided by RefSeq, Nov 2009]|
|The use of this cDNA Clones has been cited in the following citations:|
CTGF mediates tumor-stroma interactions between hepatoma cells and hepatic stellate cells to accelerate HCC progression
,Makino, Y;Hikita, H;Kodama, T;Shigekawa, M;Yamada, R;Sakamori, R;Eguchi, H;Morii, E;Yokoi, H;Mukoyama, M;Suemizu, H;Tatsumi, T;Takehara, T;,
Cancer Res. 2018
,PubMed ID 29967264
Cbx7 is epigenetically silenced in glioblastoma and inhibits cell migration by targeting YAP/TAZ-dependent transcription
,Nawaz, Z;Patil, V;Arora, A;Hegde, AS;Arivazhagan, A;Santosh, V;Somasundaram, K;,
Sci Rep Jun 2016
,PubMed ID 27291091
|cDNA Clone Resources|
|SC118973||CTGF (untagged)-Human connective tissue growth factor (CTGF)||
|RG210336||CTGF (GFP-tagged) - Human connective tissue growth factor (CTGF)||
|RC210336L1||Lenti ORF clone of Human connective tissue growth factor (CTGF), Myc-DDK-tagged||
|RC210336L2||Lenti ORF clone of Human connective tissue growth factor (CTGF), mGFP tagged||
|RC210336L3||Lenti ORF clone of Human connective tissue growth factor (CTGF), Myc-DDK-tagged||
|RC210336L4||Lenti ORF clone of Human connective tissue growth factor (CTGF), mGFP tagged||