UBASH3A (NM_018961) Human Tagged ORF Clone

CAT#: RC210190

UBASH3A (Myc-DDK-tagged)-Human ubiquitin associated and SH3 domain containing A (UBASH3A), transcript variant 1

Product Images

Circular map for RC210190

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY UBASH3A (Cat# RC210190, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UBASH3A (Cat# TA809272)(1:2000). Positive lysates LY412849 (100ug) and LC412849 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY412849) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC210190 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified UBASH3A protein (Cat# TP310190). The protein was produced from HEK293T cells transfected with UBASH3A cDNA clone (Cat# RC210190) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: UBASH3A
• Synonyms: CLIP4; STS-2; TULA; TULA-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210190 representing NM_018961
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGCGGGGGAGACGCAGCTCTACGCCAAGGTCTCCAACAAGCTCAAGGGCCGCAGCAGCCCCTCGC
  TCCTGGAGCCCCTCCTGGCCATGGGCTTCCCGGTGCACACCGCGCTGAAAGCGTTGGCAGCCACGGGGAG
  GAAGACGGCGGAGGAGGCCTTGGCCTGGCTGCATGATCATTGCAATGACCCTTCCCTAGACGACCCCATC
  CCCCAGGAGTATGCCCTTTTCCTCTGTCCAACGGGGCCCCTGCTGGAAAAACTTCAAGAGTTCTGGAGAG
  AGAGCAAGCGCCAGTGTGCAAAGAACAGAGCTCATGAGGTCTTCCCACACGTGACACTCTGTGACTTCTT
  CACGTGTGAAGACCAGAAGGTGGAATGCCTGTACGAGGCGCTGAAGAGAGCTGGAGACAGGCTCCTGGGC
  TCCTTCCCCACGGCCGTGCCTCTGGCTCTCCACTCCTCCATCAGCTACCTCGGCTTCTTCGTCAGTGGCA
  GCCCCGCAGACGTCATCCGGGAATTCGCCATGACCTTCGCCACGGAAGCATCTCTCTTAGCAGGCACTTC
  CGTTTCCCGCTTCTGGATTTTCAGCCAGGTGCCTGGACATGGCCCTAACCTGAGGCTGAGCAATTTAACT
  AGAGCCTCCTTCGTGAGCCACTACATCCTTCAAAAATACTGCTCCGTGAAGCCTTGCACCAAACAGCTGC
  ATCTGACCTTGGCCCACAAGTTCTACCCCCACCACCAGAGGACGCTGGAGCAGCTGGCCAGAGCCATCCC
  CCTGGGCCACAGCTGCCAGTGGACCGCAGCACTCTACTCCCGAGACATGCGCTTTGTGCACTACCAGACC
  CTGAGAGTCCTATTCCAGTACAAACCCCAGAACGTGGATGAGCTGACGCTAAGTCCTGGTGACTACATCT
  TTGTGGACCCCACGCAGCAGGACGAAGCCAGCGAGGGCTGGGTGATTGGGATCTCACAGCGGACGGGCTG
  CCGGGGCTTCCTGCCGGAAAACTACACGGATCGAGCCAGTGAGTCTGACACGTGGGTGAAGCACAGGATG
  TACACCTTCAGTCTAGCCACAGACCTGAACTCCAGAAAGGATGGTGAAGCCAGCAGCAGATGCAGCGGGG
  AATTTCTTCCACAAACGGCAAGGAGTCTTAGCAGCTTACAGGCCTTGCAGGCTACCGTTGCAAGGAAGAG
  TGTGCTGGTGGTTCGCCACGGGGAGAGAGTGGATCAGATCTTCGGGAAGGCATGGCTGCAGCAATGCTCC
  ACTCCTGATGGGAAATACTACAGGCCAGACCTGAATTTCCCCTGCAGTCTGCCAAGACGGAGTCGTGGGA
  TCAAAGACTTTGAAAACGATCCCCCATTATCATCGTGTGGCATTTTCCAGTCCAGAATTGCAGGGGACGC
  GCTACTGGACAGTGGTATCAGAATCAGCTCTGTGTTTGCCTCCCCAGCCCTCCGCTGTGTGCAGACGGCC
  AAACTCATCCTGGAAGAACTCAAACTGGAGAAAAAAATCAAGATACGAGTGGAACCTGGAATCTTTGAAT
  GGACAAAATGGGAAGCTGGCAAAACCACCCCAACCCTCATGAGCCTGGAAGAGCTGAAAGAGGCAAATTT
  CAACATTGACACTGATTACAGGCCCGCGTTTCCCCTGTCCGCCCTCATGCCGGCCGAGAGCTACCAGGAG
  TACATGGACAGGTGCACGGCGAGCATGGTGCAAATCGTCAACACCTGTCCACAGGACACGGGTGTCATCC
  TAATTGTGAGTCACGGCTCCACTCTGGACTCCTGCACGCGGCCACTGCTCGGGCTGCCGCCCCGGGAATG
  TGGGGATTTTGCCCAACTCGTGAGAAAGATCCCTTCCCTGGGCATGTGCTTCTGTGAAGAAAATAAAGAG
  GAAGGAAAATGGGAGTTGGTGAACCCACCGGTGAAGACCCTGACCCACGGGGCGAACGCAGCATTTAACT
  GGAGGAACTGGATCTCAGGCAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210190 representing NM_018961
  Red=Cloning site Green=Tags(s)
  
  MAAGETQLYAKVSNKLKGRSSPSLLEPLLAMGFPVHTALKALAATGRKTAEEALAWLHDHCNDPSLDDPI
  PQEYALFLCPTGPLLEKLQEFWRESKRQCAKNRAHEVFPHVTLCDFFTCEDQKVECLYEALKRAGDRLLG
  SFPTAVPLALHSSISYLGFFVSGSPADVIREFAMTFATEASLLAGTSVSRFWIFSQVPGHGPNLRLSNLT
  RASFVSHYILQKYCSVKPCTKQLHLTLAHKFYPHHQRTLEQLARAIPLGHSCQWTAALYSRDMRFVHYQT
  LRVLFQYKPQNVDELTLSPGDYIFVDPTQQDEASEGWVIGISQRTGCRGFLPENYTDRASESDTWVKHRM
  YTFSLATDLNSRKDGEASSRCSGEFLPQTARSLSSLQALQATVARKSVLVVRHGERVDQIFGKAWLQQCS
  TPDGKYYRPDLNFPCSLPRRSRGIKDFENDPPLSSCGIFQSRIAGDALLDSGIRISSVFASPALRCVQTA
  KLILEELKLEKKIKIRVEPGIFEWTKWEAGKTTPTLMSLEELKEANFNIDTDYRPAFPLSALMPAESYQE
  YMDRCTASMVQIVNTCPQDTGVILIVSHGSTLDSCTRPLLGLPPRECGDFAQLVRKIPSLGMCFCEENKE
  EGKWELVNPPVKTLTHGANAAFNWRNWISGN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018961
• ORF Size: 1983 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018961.4
• RefSeq Size: 2492 bp
• RefSeq ORF: 1986 bp
• Locus ID: 53347
• UniProt ID: P57075
• Cytogenetics: 21q22.3
• Domains: UBA, PGAM, SH3
• Protein Families: Druggable Genome
• MW: 73.9 kDa
• Gene Summary: This gene encodes one of two family members belonging to the T-cell ubiquitin ligand (TULA) family. Both family members can negatively regulate T-cell signaling. This family member can facilitate growth factor withdrawal-induced apoptosis in T cells, which may occur via its interaction with AIF, an apoptosis-inducing factor. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Aug 2011]