IL2 (NM_000586) Human Tagged ORF Clone

CAT#: RC210013L2

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  • LentiORF®

Lenti ORF clone of Human interleukin 2 (IL2), mGFP tagged



  "NM_000586" in other vectors (6)

USD 620.00


Availability*
3 Weeks

Size
    • 10 ug


Product images

Specifications

Product Data
Product Name IL2 (NM_000586) Human Tagged ORF Clone
Type Human Tagged ORF Clone
Tag mGFP
Symbol IL2
Synonyms IL-2; lymphokine; TCGF
Vector pLenti-C-mGFP
E. coli Selection Chloramphenicol (34 ug/mL)
Cell Selection None
Sequence Data
The ORF insert of this clone is exactly the same as(RC210013).
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
Tag mGFP
ACCN NM_000586
ORF Size 459 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_000586.2, NP_000577.2
RefSeq Size 822 bp
RefSeq ORF 462 bp
Locus ID 3558
Cytogenetics 4q27
Protein Families Druggable Genome, Secreted Protein
Protein Pathways Allograft rejection, Autoimmune thyroid disease, Cytokine-cytokine receptor interaction, Graft-versus-host disease, Jak-STAT signaling pathway, T cell receptor signaling pathway, Type I diabetes mellitus
MW 17.6 kDa
Gene Summary The protein encoded by this gene is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. [provided by RefSeq, Jul 2008]

Other Versions

Frequently bought together (3)
Lenti-vpak packaging kit - packaging plasmids and transfection reagent
    • 10 reactions

USD 570.00


Lenti ORF control particles of pLenti-C-mGFP-P2A-Puro, >1x10^7 TU/ml, 0.5 ml
    • 500 ul

USD 310.00


IL2 mouse monoclonal antibody,clone OTI2C9
    • 100 ul

USD 379.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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