C3orf25 (EFCAB12) (NM_207307) Human Tagged ORF Clone

CAT#: RC209876

EFCAB12 (Myc-DDK-tagged)-Human chromosome 3 open reading frame 25 (C3orf25)

Product Images

Circular map for RC209876

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY C3orf25 (Cat# RC209876, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-C3orf25 antibody (Cat# TA811971). Positive lysates LY404086 (100ug) and LC404086 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY404086) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC209876 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: C3orf25
• Synonyms: C3orf25
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209876 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGACGACTATGAAGCGTACCACAGTCTGTTCTTGTCGCTGCTCGGACTCTGCCCGTCTAAGACTC
  CCATCAATGAAAATGCTCCCGTCTTTGATCCTGAACCGGTCATTGCCCACTGCTTCAAGCAGTTCCAGCA
  GAAGGACTTCCGCCTGCCTCAGACCCGCCGGCGAATCATCATGGTGCCTCGCAAGGAGGATCAGACACCC
  CTTAATCCTGCATCCCAACCTCAGGCTCCCCCAAAGCCCATCCCCAGCTTCAAAGTTCTGGAAGCTAGAG
  ATATCCAAGAGCAGCCAGAGGACAGGAAGACCTGGCTGAGCCAGAGGTCGAAGCTGCGGCAGGAGCTAGA
  GTCCTTTGGTGATGTAAAGAGGTGGCTGGAGAACAAGCCCAGCATCACGCCTTCAGAGGCCAAGGTCTTA
  CACATGATCCACGAGGAGCAGAGTGCCCAGCCAAATGCCTCCCAGGCAACTACCAGGACCACCAGGAAGA
  AAGCCCCCAGGCTCTCCCGGCTGTCCCGCCAGATGGTGCCCCAGCTCCAGCTGCCCGAGCCCCCTGCCCT
  GTCGGTCATGTACTCCTACCTGCATAGCCGCAAGATCAAGATCCTGGAGATATTTCACAAGGTGGGCCAG
  GGTGAGAACCAGAGAATCACCAGGGAGGAGTTCATCGCGGCTGTAAAGGCAGTCGGAGTCCCTCTGAAGA
  ACCAAGAGGTGGAGGATATAGTGATCTACCTCAGCTCTCTTGGGAAGCACAACACCATCACCATGGATAT
  CCTGGCCAATACCTACAAGCAGTGGTCTATGGCTCAGCAAAGGAGCAGCCTGGCCACTGCAAGGGAGCAT
  TATATCTTGGCCAAGCACAGAGATTCCCTGAAGGGTCCGCTCAAGAAGCAGGAGGTGGATTCAGCCCCAC
  AGCTTCCCAAAGTGGACCTACTGACGGTGCCTGCAGTCGACACGCAGATGGAGACGCGGCCCATGACCCT
  GGAGGAGATGGAGGAAGTGGGCAAGCGGTACCGCGAGCGGCAGCGACAGCACAAGCTCACGATCCCCTCC
  ATCCAGTACACGGAGCAATGTCACCTGGTGCGCTGTGGGAATCGGCACTTTGATGAGCACTGCCTCCCGT
  CCACCATCCACGGGGATATGAGGGAGCTCATTGACTCGGCCCGCAGGCACAACTTTCTGGTCTACCTGCA
  ATGCTGGAAGCTCTGTAAGTCCTATGGCCTCCCGCTGACAGAGGACATCCTCATGAAAGCCTTGCTGTAC
  CCAGGAGACAAGATCATTTTCCAGATGGACAAAGTGTGCCCCATCCGGCAGCCGGGAGGCTACTACTCTG
  ACTGGAAGGTCTTTTCTCCGAATCTGGCTCTGCTCCGGTCCCAGGGCCCTGGCAAGTCTAAGAGGACTGA
  CAAGAAAACGCCAAAGAAAAGCAAGAAAATGCGCTTTAAGGAGTTTGAGGAATTTACCAGGAAGCTGAAG
  GTGAAGAGGTCCAGTGGTCTGCAGCAAACACACCCCAATTCCTTCTGGCCGGGTCATCTTCTGGATAAGC
  TGCAGCTCTACCTGCCCACTGTGGCCACAGACCGGAGCCTGGCGCTCTTCAGTTGTGTTCAACACCAGCC
  CCATGTCTACCCAGCCACCTACCACCCTGACCACTGGTGGCCCCTTAGGAACAAGAACTACATGACCCAC
  GCCCATTATGATGCCGCCAAGGTGTACTACATCAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209876 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDDDYEAYHSLFLSLLGLCPSKTPINENAPVFDPEPVIAHCFKQFQQKDFRLPQTRRRIIMVPRKEDQTP
  LNPASQPQAPPKPIPSFKVLEARDIQEQPEDRKTWLSQRSKLRQELESFGDVKRWLENKPSITPSEAKVL
  HMIHEEQSAQPNASQATTRTTRKKAPRLSRLSRQMVPQLQLPEPPALSVMYSYLHSRKIKILEIFHKVGQ
  GENQRITREEFIAAVKAVGVPLKNQEVEDIVIYLSSLGKHNTITMDILANTYKQWSMAQQRSSLATAREH
  YILAKHRDSLKGPLKKQEVDSAPQLPKVDLLTVPAVDTQMETRPMTLEEMEEVGKRYRERQRQHKLTIPS
  IQYTEQCHLVRCGNRHFDEHCLPSTIHGDMRELIDSARRHNFLVYLQCWKLCKSYGLPLTEDILMKALLY
  PGDKIIFQMDKVCPIRQPGGYYSDWKVFSPNLALLRSQGPGKSKRTDKKTPKKSKKMRFKEFEEFTRKLK
  VKRSSGLQQTHPNSFWPGHLLDKLQLYLPTVATDRSLALFSCVQHQPHVYPATYHPDHWWPLRNKNYMTH
  AHYDAAKVYYIN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_207307
• ORF Size: 1716 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_207307.3
• RefSeq Size: 2169 bp
• RefSeq ORF: 1719 bp
• Locus ID: 90288
• UniProt ID: Q6NXP0
• Cytogenetics: 3q21.3
• MW: 66.6 kDa