Protamine 2 (PRM2) (NM_002762) Human Tagged ORF Clone
CAT#: RC209843
PRM2 (Myc-DDK-tagged)-Human protamine 2 (PRM2)
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_002762" in other vectors (4)
USD 198.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Protamine 2 |
Synonyms | CT94.2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC209843 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTCCGATACCGCGTGAGGAGCCTGAGCGAACGCTCGCACGAGGTGTACAGGCAGCAGTTGCATGGGC AAGAGCAAGGACACCACGGCCAAGAGGAGCAAGGGCTGAGCCCGGAGCACGTCGAGGTCTACGAGAGGAC CCATGGCCAGTCTCACTATAGGCGCAGACACTGCTCTCGAAGGAGGCTGCACCGGATCCACAGGCGGCAG CATCGCTCCTGCAGAAGGCGCAAAAGACGCTCCTGCAGGCACCGGAGGAGGCATCGCAGAGGCTGCAGAA CCAGGAAGAGAACATGCAGAAGGCAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC209843 protein sequence
Red=Cloning site Green=Tags(s) MVRYRVRSLSERSHEVYRQQLHGQEQGHHGQEEQGLSPEHVEVYERTHGQSHYRRRHCSRRRLHRIHRRQ HRSCRRRKRRSCRHRRRHRRGCRTRKRTCRRH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_002762 |
ORF Size | 306 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_002762.4 |
RefSeq Size | 683 bp |
RefSeq ORF | 309 bp |
Locus ID | 5620 |
UniProt ID | P04554 |
Cytogenetics | 16p13.13 |
MW | 13.1 kDa |
Gene Summary | Protamines substitute for histones in the chromatin of sperm during the haploid phase of spermatogenesis, and are the major DNA-binding proteins in the nucleus of sperm in many vertebrates. They package the sperm DNA into a highly condensed complex in a volume less than 5% of a somatic cell nucleus. Many mammalian species have only one protamine (protamine 1); however, a few species, including human and mouse, have two. This gene encodes protamine 2, which is cleaved to give rise to a family of protamine 2 peptides. Alternatively spliced transcript variants have also been found for this gene. [provided by RefSeq, Sep 2015] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC209843L3 | Lenti ORF clone of Human protamine 2 (PRM2), Myc-DDK-tagged |
USD 450.00 |
|
RC209843L4 | Lenti ORF clone of Human protamine 2 (PRM2), mGFP tagged |
USD 450.00 |
|
RG209843 | PRM2 (tGFP-tagged) - Human protamine 2 (PRM2) |
USD 350.00 |
|
SC125724 | PRM2 (untagged)-Human protamine 2 (PRM2) |
USD 150.00 |
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