BPNT2 (NM_017813) Human Tagged ORF Clone

CAT#: RC209799

IMPAD1 (Myc-DDK-tagged)-Human inositol monophosphatase domain containing 1 (IMPAD1)

Product Images

Circular map for RC209799

• 

  Western blot validation of overexpression lysate (Cat# LY413522) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC209799 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BPNT2
• Synonyms: GPAPP; IMP-3; IMP 3; IMPA3; IMPAD1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209799 representing NM_017813
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCCCATGGGCATCCGCCTTTCCCCACTGGGGGTGGCAGTGTTTTGCCTGCTGGGGCTCGGCGTGC
  TCTACCACCTCTACTCGGGCTTCTTGGCCGGCCGCTTCAGCCTCTTCGGCCTGGGCGGCGAGCCTGGCGG
  CGGCGCGGCGGGGCCCGCGGCCGCGGCCGATGGGGGCACCGTGGACTTGCGCGAGATGCTGGCTGTGTCA
  GTGCTGGCCGCAGTCCGCGGCGGCGACGAGGTGAGGCGCGTCCGCGAGAGCAACGTCCTCCACGAGAAGT
  CCAAGGGGAAGACGCGCGAGGGAGCCGAGGACAAGATGACCAGCGGCGACGTGCTGTCCAACCGCAAGAT
  GTTCTACCTGCTCAAGACCGCCTTCCCCAGCGTCCAGATTAATACTGAGGAACACGTGGATGCAGCTGAT
  CAGGAGGTTATCTTGTGGGATCATAAGATTCCTGAGGATATCCTAAAGGAAGTAACTACTCCTAAAGAGG
  TACCAGCAGAAAGTGTTACTGTCTGGATTGACCCACTTGATGCTACACAGGAATATACAGAGGATCTTCG
  AAAGTACGTCACTACTATGGTGTGTGTGGCTGTAAATGGTAAACCCATGCTAGGAGTTATACATAAGCCA
  TTTTCCGAATATACAGCTTGGGCAATGGTAGATGGTGGTTCAAATGTGAAAGCCCGCTCTTCCTACAATG
  AGAAGACCCCAAGGATCGTTGTGTCTCGTTCCCATTCAGGGATGGTCAAACAGGTCGCTCTTCAGACTTT
  TGGAAACCAGACTACAATTATCCCAGCTGGTGGTGCTGGTTATAAAGTTTTAGCACTTTTGGATGTGCCT
  GATAAGAGTCAAGAAAAAGCTGATTTATACATCCATGTGACATACATCAAAAAGTGGGATATATGTGCTG
  GTAATGCCATCTTAAAAGCCCTAGGGGGGCATATGACTACCCTGAGTGGTGAAGAAATCAGTTACACTGG
  TTCAGACGGCATTGAAGGGGGACTCCTTGCTAGCATCAGAATGAACCACCAGGCCCTGGTCAGAAAACTC
  CCAGATCTAGAAAAGACAGGACATAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209799 representing NM_017813
  Red=Cloning site Green=Tags(s)
  
  MAPMGIRLSPLGVAVFCLLGLGVLYHLYSGFLAGRFSLFGLGGEPGGGAAGPAAAADGGTVDLREMLAVS
  VLAAVRGGDEVRRVRESNVLHEKSKGKTREGAEDKMTSGDVLSNRKMFYLLKTAFPSVQINTEEHVDAAD
  QEVILWDHKIPEDILKEVTTPKEVPAESVTVWIDPLDATQEYTEDLRKYVTTMVCVAVNGKPMLGVIHKP
  FSEYTAWAMVDGGSNVKARSSYNEKTPRIVVSRSHSGMVKQVALQTFGNQTTIIPAGGAGYKVLALLDVP
  DKSQEKADLYIHVTYIKKWDICAGNAILKALGGHMTTLSGEEISYTGSDGIEGGLLASIRMNHQALVRKL
  PDLEKTGHK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017813
• ORF Size: 1077 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017813.5
• RefSeq Size: 2438 bp
• RefSeq ORF: 1080 bp
• Locus ID: 54928
• UniProt ID: Q9NX62
• Cytogenetics: 8q12.1
• Domains: inositol_P
• Protein Families: Transmembrane
• MW: 38.5 kDa
• Gene Summary: This gene encodes a member of the inositol monophosphatase family. The encoded protein is localized to the Golgi apparatus and catalyzes the hydrolysis of phosphoadenosine phosphate (PAP) to adenosine monophosphate (AMP). Mutations in this gene are a cause of GRAPP type chondrodysplasia with joint dislocations, and a pseudogene of this gene is located on the long arm of chromosome 1. [provided by RefSeq, Dec 2011]