GBP4 (NM_052941) Human Tagged ORF Clone

CAT#: RC209769

GBP4 (Myc-DDK-tagged)-Human guanylate binding protein 4 (GBP4)

Product Images

Circular map for RC209769

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  Western blot validation of overexpression lysate (Cat# LY409376) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC209769 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified GBP4 protein (Cat# TP309769). The protein was produced from HEK293T cells transfected with GBP4 cDNA clone (Cat# RC209769) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GBP4
• Synonyms: Mpa2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209769 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTGAGAGAACTCTTCACGCTGCAGTGCCCACACCAGGTTATCCAGAATCTGAATCCATCATGATGG
  CCCCCATTTGTCTAGTGGAAAACCAGGAAGAGCAGCTGACAGTGAATTCAAAGGCATTAGAGATTCTTGA
  CAAGATTTCTCAGCCCGTGGTGGTGGTGGCCATTGTAGGGCTATACCGCACAGGAAAATCCTATCTCATG
  AATCGTCTTGCAGGAAAGCGCAATGGCTTCCCTCTGGGCTCCACGGTGCAGTCTGAAACTAAGGGCATCT
  GGATGTGGTGTGTGCCCCACCTCTCTAAGCCAAACCACACCCTGGTCCTTCTGGACACCGAGGGCCTGGG
  CGATGTAGAAAAGAGTAACCCTAAGAATGACTCGTGGATCTTTGCCCTGGCTGTGCTTCTAAGCAGCAGC
  TTTGTCTATAACAGCGTGAGCACCATCAACCACCAGGCCCTGGAGCAGCTGCACTATGTGACTGAGCTAG
  CAGAGCTAATCAGGGCAAAATCCTGCCCCAGACCTGATGAAGCTGAGGACTCCAGCGAGTTTGCGAGTTT
  CTTTCCAGACTTTATTTGGACTGTTCGGGATTTTACCCTGGAGCTAAAGTTAGATGGAAACCCCATCACA
  GAAGATGAGTACCTGGAGAATGCCTTGAAGCTGATTCCAGGCAAGAATCCCAAAATTCAAAATTCAAACA
  TGCCTAGAGAGTGTATCAGGCATTTCTTCCGAAAACGGAAGTGCTTTGTCTTTGACCGGCCTACAAATGA
  CAAGCAATATTTAAATCATATGGACGAAGTGCCAGAAGAAAATCTGGAAAGGCATTTCCTTATGCAATCA
  GACAACTTCTGTTCTTATATCTTCACCCATGCAAAGACCAAGACCCTGAGAAAGGGAATCATTGTCACTG
  GAAAGCGGCTGGGGACTCTGGTGGTGACTTATGTAGATGCCATCAACAGTGGAGCAGTACCTTGTCTGGA
  GAATGCAGTGACAGCACTGGCCCAGCTTGAGAACCCAGCGGCTGTGCAGAGGGCAGCCGACCACTATAGC
  CAGCAGATGGCCCAGCAACTGAGGCTCCCCACAGACACGCTCCAGGAGCTGCTGGACGTGCATGCAGCCT
  GTGAGAGGGAAGCCATTGCAGTCTTCATGGAGCACTCCTTCAAGGATGAAAACCATGAATTCCAGAAGAA
  GCTTGTGGACACCATAGAGAAAAAGAAGGGAGACTTTGTGCTGCAGAATGAAGAGGCATCTGCCAAATAT
  TGCCAGGCTGAGCTTAAGCGGCTTTCAGAGCACCTGACAGAAAGCATTTTGAGAGGAATTTTCTCTGTTC
  CTGGAGGACACAATCTCTACTTAGAAGAAAAGAAACAGGTTGAGTGGGACTATAAGCTAGTGCCCAGAAA
  AGGAGTTAAGGCAAACGAGGTCCTCCAGAACTTCCTGCAGTCACAGGTGGTTGTAGAGGAATCCATCCTG
  CAGTCAGACAAAGCCCTCACTGCTGGAGAGAAGTCCATAGCAGCGGAGCGGGCCATGAAGGAAGCAGCTG
  AGAAGGAACAGGAGCTGCTAAGAGAAAAACAGAAGGAGCAGCAGCAAATGATGGAGGCTCAAGAGAGAAG
  CTTCCAGGAATACATGGCCCAAATGGAGAAGAAGTTGGAGGAGGAAAGGGAAAACCTTCTCAGAGAGCAT
  GAAAGGCTGCTAAAACACAAGCTGAAGGTACAAGAAGAAATGCTTAAGGAAGAATTTCAAAAGAAATCTG
  AGCAGTTAAATAAAGAGATTAATCAACTGAAAGAAAAAATTGAAAGCACTAAAAATGAACAGTTAAGGCT
  CTTAAAGATCCTTGACATGGCTAGCAACATAATGATTGTCACTCTACCTGGGGCTTCCAAGCTACTTGGA
  GTAGGGACAAAATATCTTGGCTCACGTATT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209769 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGERTLHAAVPTPGYPESESIMMAPICLVENQEEQLTVNSKALEILDKISQPVVVVAIVGLYRTGKSYLM
  NRLAGKRNGFPLGSTVQSETKGIWMWCVPHLSKPNHTLVLLDTEGLGDVEKSNPKNDSWIFALAVLLSSS
  FVYNSVSTINHQALEQLHYVTELAELIRAKSCPRPDEAEDSSEFASFFPDFIWTVRDFTLELKLDGNPIT
  EDEYLENALKLIPGKNPKIQNSNMPRECIRHFFRKRKCFVFDRPTNDKQYLNHMDEVPEENLERHFLMQS
  DNFCSYIFTHAKTKTLRKGIIVTGKRLGTLVVTYVDAINSGAVPCLENAVTALAQLENPAAVQRAADHYS
  QQMAQQLRLPTDTLQELLDVHAACEREAIAVFMEHSFKDENHEFQKKLVDTIEKKKGDFVLQNEEASAKY
  CQAELKRLSEHLTESILRGIFSVPGGHNLYLEEKKQVEWDYKLVPRKGVKANEVLQNFLQSQVVVEESIL
  QSDKALTAGEKSIAAERAMKEAAEKEQELLREKQKEQQQMMEAQERSFQEYMAQMEKKLEEERENLLREH
  ERLLKHKLKVQEEMLKEEFQKKSEQLNKEINQLKEKIESTKNEQLRLLKILDMASNIMIVTLPGASKLLG
  VGTKYLGSRI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_052941
• ORF Size: 1920 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_052941.5
• RefSeq Size: 6160 bp
• RefSeq ORF: 1923 bp
• Locus ID: 115361
• UniProt ID: Q96PP9
• Cytogenetics: 1p22.2
• Domains: GBP
• MW: 73.2 kDa
• Gene Summary: Guanylate-binding proteins, such as GBP4, are induced by interferon and hydrolyze GTP to both GDP and GMP (Vestal, 2005 [PubMed 16108726]).[supplied by OMIM, Dec 2008]