LRP15 (LRRC3B) (NM_052953) Human Tagged ORF Clone
LRRC3B (Myc-DDK-tagged)-Human leucine rich repeat containing 3B (LRRC3B)
|Type||Human Tagged ORF Clone|
|E. coli Selection||Kanamycin (25 ug/mL)|
|Mammalian Cell Selection||Neomycin|
>RC209766 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)
>RC209766 protein sequence
Red=Cloning site Green=Tags(s)
Sequencher program is needed, download here.
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||777 bp|
|OTI Disclaimer||The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info|
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq Size||1718 bp|
|RefSeq ORF||780 bp|
|Domains||LRRNT, LRR, LRR_TYP|
|Gene Summary||The protein encoded by this gene is a tumor suppressor, with lowered expression levels found in gastric, renal, colorectal, lung, and breast cancer tissues. The promoter of this gene is frequently hypermethylated in these cancer tissues, although the hypermethylation does not appear to be the cause of the reduced expression of this gene. Several transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Dec 2015]|
|cDNA Clone Resources|
|RC209766L3||Lenti ORF clone of Human leucine rich repeat containing 3B (LRRC3B), Myc-DDK-tagged||
|RC209766L4||Lenti ORF clone of Human leucine rich repeat containing 3B (LRRC3B), mGFP tagged||
|RG209766||LRRC3B (GFP-tagged) - Human leucine rich repeat containing 3B (LRRC3B)||
|SC120172||LRRC3B (untagged)-Human leucine rich repeat containing 3B (LRRC3B)||