C5L2 (C5AR2) (NM_018485) Human Tagged ORF Clone

CAT#: RC209676

C5AR2 (Myc-DDK-tagged)-Human G protein-coupled receptor 77 (GPR77)

Product Images

Circular map for RC209676

• 

  Western blot validation of overexpression lysate (Cat# LY402691) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC209676 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: C5L2
• Synonyms: C5L2; GPF77; GPR77
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209676 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGAACGATTCTGTCAGCTACGAGTATGGGGATTACAGCGACCTCTCGGACCGCCCTGTGGACTGCC
  TGGATGGCGCCTGCCTGGCCATCGACCCGCTGCGCGTGGCCCCGCTCCCACTGTATGCCGCCATCTTCCT
  GGTGGGGGTGCCGGGCAATGCCATGGTGGCCTGGGTGGCTGGGAAGGTGGCCCGCCGGAGGGTGGGTGCC
  ACCTGGTTGCTCCACCTGGCCGTGGCGGATTTGCTGTGCTGTTTGTCTCTGCCCATCCTGGCAGTGCCCA
  TTGCCCGTGGAGGCCACTGGCCGTATGGTGCAGTGGGCTGTCGGGCGCTGCCCTCCATCATCCTGCTGAC
  CATGTATGCCAGCGTCCTGCTCCTGGCAGCTCTCAGTACCGACCTCTGCTTCCTGGCTCTCGGGCCTGCC
  TGGTGGTCTACGGTTCAGCGGGCGTGCGGGGTGCAGGTGGCCTGTGGGGCAGCCTGGACACTGGCCTTGC
  TGCTCACCGTGCCCTCCGCCATCTACCGCCGGCTGCACCAGGAGCACTTCCCAGCCCGGCTGCAGTGTGT
  GGTGGACTACGGCGGCTCCTCCAGCACCGAGAATGCGGTGACTGCCATCCGGTTTCTTTTTGGCTTCCTG
  GGGCCCCTGGTGGCCGTGGTCAGCTGCCACAGTGCCCTCCTGTGCTGGGCAGCCCGACGCTGCCGGCCGC
  TGGGCACAGCCATTGTGGTGGGGTTTTTTGTCTGCTGGGCACCCTACCACCTGCTGGGGCTGGTGCTCAC
  TGTGGCGGCCCCGAACTCCGCACTCCTGGCCAGGGCCCTGCGGGCTGAACCCCTCATCGTGGGCCTTGCC
  CTCGCTCACAGCTGCCTCAATCCCATGCTCTTCCTGTATTTTGGGAGGGCTCAACTCCGCCGGTCACTGC
  CAGCTGCCTGTCACTGGGCCCTGAGGGAGTCCCAGGGCCAGGACGAAAGTGTGGACAGCAAGAAATCCAC
  CAGCCATGACCTGGTCTCGGAGATGGAGGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209676 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGNDSVSYEYGDYSDLSDRPVDCLDGACLAIDPLRVAPLPLYAAIFLVGVPGNAMVAWVAGKVARRRVGA
  TWLLHLAVADLLCCLSLPILAVPIARGGHWPYGAVGCRALPSIILLTMYASVLLLAALSTDLCFLALGPA
  WWSTVQRACGVQVACGAAWTLALLLTVPSAIYRRLHQEHFPARLQCVVDYGGSSSTENAVTAIRFLFGFL
  GPLVAVVSCHSALLCWAARRCRPLGTAIVVGFFVCWAPYHLLGLVLTVAAPNSALLARALRAEPLIVGLA
  LAHSCLNPMLFLYFGRAQLRRSLPAACHWALRESQGQDESVDSKKSTSHDLVSEMEV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018485
• ORF Size: 1011 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018485.3
• RefSeq Size: 1396 bp
• RefSeq ORF: 1014 bp
• Locus ID: 27202
• UniProt ID: Q9P296
• Cytogenetics: 19q13.32
• Domains: 7tm_1
• Protein Families: Druggable Genome, GPCR, Transmembrane
• MW: 36.1 kDa
• Gene Summary: This gene encodes a G-protein coupled receptor 1 family member involved in the complement system of the innate immune response. Unlike classical G-protein coupled receptors, the encoded protein does not associate with intracellular G-proteins. It may instead modulate signal transduction through the beta-arrestin pathway, and may alternatively act as a decoy receptor. This gene may be involved in coronary artery disease and in the pathogenesis of sepsis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2012]