ALDH1A3 (NM_000693) Human Tagged ORF Clone

CAT#: RC209656

ALDH1A3 (Myc-DDK-tagged)-Human aldehyde dehydrogenase 1 family, member A3 (ALDH1A3)

Product Images

Circular map for RC209656

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  Western blot validation of overexpression lysate (Cat# LY400233) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC209656 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY ALDH1A3 (Cat# RC209656, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ALDH1A3 (Cat# TA502841)(1:500).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ALDH1A3
• Synonyms: ALDH1A6; ALDH6; MCOP8; RALDH3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209656 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCACCGCTAACGGGGCCGTGGAAAACGGGCAGCCGGACAGGAAGCCGCCGGCCCTGCCGCGCCCCA
  TCCGCAACCTGGAGGTCAAGTTCACCAAGATATTTATCAACAATGAATGGCACGAATCCAAGAGTGGGAA
  AAAGTTTGCTACATGTAACCCTTCAACTCGGGAGCAAATATGTGAAGTGGAAGAAGGAGATAAGCCCGAC
  GTGGACAAGGCTGTGGAGGCTGCACAGGTTGCCTTCCAGAGGGGCTCGCCATGGCGCCGGCTGGATGCCC
  TGAGTCGTGGGCGGCTGCTGCACCAGCTGGCTGACCTGGTGGAGAGGGACCGCGCCACCTTGGCCGCCCT
  GGAGACGATGGATACAGGGAAGCCATTTCTTCATGCTTTTTTCATCGACCTGGAGGGCTGTATTAGAACC
  CTCAGATACTTTGCAGGGTGGGCAGACAAAATCCAGGGCAAGACCATCCCCACAGATGACAACGTCGTGT
  GCTTCACCAGGCATGAGCCCATTGGTGTCTGTGGGGCCATCACTCCATGGAACTTCCCCCTGCTGATGCT
  GGTGTGGAAGCTGGCACCCGCCCTCTGCTGTGGGAACACCATGGTCCTGAAGCCTGCGGAGCAGACACCT
  CTCACCGCCCTTTATCTCGGCTCTCTGATCAAAGAGGCCGGGTTCCCTCCAGGAGTGGTGAACATTGTGC
  CAGGATTCGGGCCCACAGTGGGAGCAGCAATTTCTTCTCACCCTCAGATCAACAAGATCGCCTTCACCGG
  CTCCACAGAGGTTGGAAAACTGGTTAAAGAAGCTGCGTCCCGGAGCAATCTGAAGCGGGTGACGCTGGAG
  CTGGGGGGGAAGAACCCCTGCATCGTGTGTGCGGACGCTGACTTGGACTTGGCAGTGGAGTGTGCCCATC
  AGGGAGTGTTCTTCAACCAAGGCCAGTGTTGCACGGCAGCCTCCAGGGTGTTCGTGGAGGAGCAGGTCTA
  CTCTGAGTTTGTCAGGCGGAGCGTGGAGTATGCCAAGAAACGGCCCGTGGGAGACCCCTTCGATGTCAAA
  ACAGAACAGGGGCCTCAGATTGATCAAAAGCAGTTCGACAAAATCTTAGAGCTGATCGAGAGTGGGAAGA
  AGGAAGGGGCCAAGCTGGAATGCGGGGGCTCAGCCATGGAAGACAAGGGGCTCTTCATCAAACCCACTGT
  CTTCTCAGAAGTCACAGACAACATGCGGATTGCCAAAGAGGAGATTTTCGGGCCAGTGCAACCAATACTG
  AAGTTCAAAAGTATCGAAGAAGTGATAAAAAGAGCGAATAGCACCGACTATGGACTCACAGCAGCCGTGT
  TCACAAAAAATCTCGACAAAGCCCTGAAGTTGGCTTCTGCCTTAGAGTCTGGAACGGTCTGGATCAACTG
  CTACAACGCCCTCTATGCACAGGCTCCATTTGGTGGCTTTAAAATGTCAGGAAATGGCAGAGAACTAGGT
  GAATACGCTTTGGCCGAATACACAGAAGTGAAAACTGTCACCATCAAACTTGGCGACAAGAACCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209656 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MATANGAVENGQPDRKPPALPRPIRNLEVKFTKIFINNEWHESKSGKKFATCNPSTREQICEVEEGDKPD
  VDKAVEAAQVAFQRGSPWRRLDALSRGRLLHQLADLVERDRATLAALETMDTGKPFLHAFFIDLEGCIRT
  LRYFAGWADKIQGKTIPTDDNVVCFTRHEPIGVCGAITPWNFPLLMLVWKLAPALCCGNTMVLKPAEQTP
  LTALYLGSLIKEAGFPPGVVNIVPGFGPTVGAAISSHPQINKIAFTGSTEVGKLVKEAASRSNLKRVTLE
  LGGKNPCIVCADADLDLAVECAHQGVFFNQGQCCTAASRVFVEEQVYSEFVRRSVEYAKKRPVGDPFDVK
  TEQGPQIDQKQFDKILELIESGKKEGAKLECGGSAMEDKGLFIKPTVFSEVTDNMRIAKEEIFGPVQPIL
  KFKSIEEVIKRANSTDYGLTAAVFTKNLDKALKLASALESGTVWINCYNALYAQAPFGGFKMSGNGRELG
  EYALAEYTEVKTVTIKLGDKNP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000693
• ORF Size: 1536 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000693.4
• RefSeq Size: 3622 bp
• RefSeq ORF: 1539 bp
• Locus ID: 220
• UniProt ID: P47895
• Cytogenetics: 15q26.3
• Domains: aldedh
• Protein Families: Druggable Genome
• Protein Pathways: Drug metabolism - cytochrome P450, Glycolysis / Gluconeogenesis, Histidine metabolism, Metabolic pathways, Metabolism of xenobiotics by cytochrome P450, Phenylalanine metabolism, Tyrosine metabolism
• MW: 56.1 kDa
• Gene Summary: This gene encodes an aldehyde dehydrogenase enzyme that uses retinal as a substrate. Mutations in this gene have been associated with microphthalmia, isolated 8, and expression changes have also been detected in tumor cells. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2014]