CENPL (NM_033319) Human Tagged ORF Clone

CAT#: RC209587

CENPL (Myc-DDK-tagged)-Human centromere protein L (CENPL), transcript variant 2

Product Images

Circular map for RC209587

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  Western blot validation of overexpression lysate (Cat# LY409603) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC209587 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified CENPL protein (Cat# TP309587). The protein was produced from HEK293T cells transfected with CENPL cDNA clone (Cat# RC209587) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CENPL
• Synonyms: C1orf155; CENP-L; dJ383J4.3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209587 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATTCTTACAGTGCACCAGAGTCAACTCCTAGTGCATCCTCAAGACCTGAAGATTACTTTATAGGTG
  CCACTCCTCTGCAGAAACGATTAGAATCGGTCAGGAAGCAGAGTTCATTTATCCTGACTCCACCTCGAAG
  GAAAATTCCCCAGTGTTCGCAGTTGCAGGAAGATGTTGACCCTCAAAAGGTTGCATTCCTTCTGCATAAA
  CAGTGGACTTTATATAGTTTAACTCCCTTATATAAATTCTCCTATAGTAATCTCAAAGAGTATTCTAGAC
  TTCTCAATGCTTTTATTGTTGCTGAAAAGCAAAAAGGACTTGCTGTGGAAGTGGGAGAAGACTTCAACAT
  CAAAGTGATTTTTTCTACTCTCCTAGGAATGAAAGGAACACAAAGGGACCCGGAAGCATTTCTTGTCCAG
  ATTGTGTCAAAATCTCAATTGCCATCTGAGAATAGAGAAGGTAAAGTGCTGTGGACTGGCTGGTTCTGCT
  GTGTATTTGGAGACAGTCTTCTGGAGACTGTTTCAGAAGATTTCACCTGTCTGCCCTTATTCCTTGCAAA
  TGGAGCAGAGTCTAACACAGCAATAATTGGAACTTGGTTTCAGAAAACCTTTGACTGTTATTTCAGTCCT
  TTAGCAATCAATGCATTTAATCTTTCCTGGATGGCTGCCATGTGGACTGCATGCAAAATGGACCATTATG
  TGGCTACTACTGAATTTCTTTGGTCTGTACCCTGTAGCCCTCAAAGTCTGGACATTTCTTTCGCAATACA
  TCCAGAGGATGCAAAAGCTCTATGGGACAGTGTCCACAAAACACCTGGGGAGGTTACCCAGGAAGAAGTT
  GACCTATTCATGGATTGCCTTTATTCACATTTCCATAGACATTTCAAAATTCATTTATCAGCCACAAGAT
  TAGTTCGTGTTTCAACATCTGTAGCTTCAGCACATACTGATGGAAAAATAAAGATTCTGTGTCATAAATA
  CCTTATTGGAGTGTTAGCATATTTGACAGAACTGGCAATTTTTCAAATTGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209587 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDSYSAPESTPSASSRPEDYFIGATPLQKRLESVRKQSSFILTPPRRKIPQCSQLQEDVDPQKVAFLLHK
  QWTLYSLTPLYKFSYSNLKEYSRLLNAFIVAEKQKGLAVEVGEDFNIKVIFSTLLGMKGTQRDPEAFLVQ
  IVSKSQLPSENREGKVLWTGWFCCVFGDSLLETVSEDFTCLPLFLANGAESNTAIIGTWFQKTFDCYFSP
  LAINAFNLSWMAAMWTACKMDHYVATTEFLWSVPCSPQSLDISFAIHPEDAKALWDSVHKTPGEVTQEEV
  DLFMDCLYSHFHRHFKIHLSATRLVRVSTSVASAHTDGKIKILCHKYLIGVLAYLTELAIFQIE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_033319
• ORF Size: 1032 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_033319.3, NP_201576.1
• RefSeq Size: 2555 bp
• RefSeq ORF: 1035 bp
• Locus ID: 91687
• UniProt ID: Q8N0S6
• Cytogenetics: 1q25.1
• MW: 39 kDa
• Gene Summary: CENPL is a subunit of a CENPH (MIM 605607)-CENPI (MIM 300065)-associated centromeric complex that targets CENPA (MIM 117139) to centromeres and is required for proper kinetochore function and mitotic progression (Okada et al., 2006) [PubMed 16622420].[supplied by OMIM, Mar 2008]