Complement C7 (C7) (NM_000587) Human Tagged ORF Clone

CAT#: RC209562

C7 (Myc-DDK-tagged)-Human complement component 7 (C7)

Product Images

Circular map for RC209562

• 

  Western blot validation of overexpression lysate (Cat# LY424620) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC209562 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Complement C7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209562 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGGTGATAAGCTTATTCATTTTGGTGGGATTTATAGGAGAGTTCCAAAGTTTTTCAAGTGCCTCCT
  CTCCAGTCAACTGCCAGTGGGACTTCTATGCCCCTTGGTCAGAATGCAATGGCTGTACCAAGACTCAGAC
  TCGCAGGCGGTCAGTTGCTGTGTATGGGCAGTATGGAGGCCAGCCTTGTGTTGGAAATGCTTTTGAAACA
  CAGTCCTGTGAACCTACAAGAGGATGTCCAACAGAGGAGGGATGTGGAGAGCGTTTCAGGTGCTTTTCAG
  GTCAGTGCATCAGCAAATCATTGGTTTGCAATGGGGATTCTGACTGTGATGAAGACAGTGCTGATGAAGA
  CAGATGTGAGGACTCAGAAAGGAGACCTTCCTGTGATATCGATAAACCTCCTCCTAACATAGAACTTACT
  GGAAATGGTTACAATGAACTCACTGGCCAGTTTAGGAACAGAGTCATCAATACCAAAAGTTTTGGTGGTC
  AATGTAGAAAGGTGTTTAGTGGGGATGGAAAAGATTTCTACAGGCTGAGTGGAAATGTCCTGTCCTATAC
  ATTCCAGGTGAAAATAAATAATGATTTTAATTATGAATTTTACAATAGTACTTGGTCTTATGTAAAACAT
  ACGTCGACAGAACACACATCATCTAGTCGGAAGCGCTCCTTTTTTAGATCTTCATCATCTTCTTCACGCA
  GTTATACTTCACATACCAATGAAATCCATAAAGGAAAGAGTTACCAACTGCTGGTTGTTGAGAACACTGT
  TGAAGTGGCTCAGTTCATTAATAACAATCCAGAATTTTTACAACTTGCTGAGCCATTCTGGAAGGAGCTT
  TCCCACCTCCCCTCTCTGTATGACTACAGTGCCTACCGAAGATTAATCGACCAGTACGGGACACATTATC
  TGCAATCTGGGTCGTTAGGAGGAGAATACAGAGTTCTATTTTATGTGGACTCAGAAAAATTAAAACAAAA
  TGATTTTAATTCAGTCGAAGAAAAGAAATGTAAATCCTCAGGTTGGCATTTTGTCGTTAAATTTTCAAGT
  CATGGATGCAAGGAACTGGAAAACGCTTTAAAAGCTGCTTCAGGAACCCAGAACAATGTATTGCGAGGAG
  AACCGTTCATCAGAGGGGGAGGTGCAGGCTTCATATCTGGCCTTAGTTACCTAGAGCTGGACAATCCTGC
  TGGAAACAAAAGGCGATATTCTGCCTGGGCAGAATCTGTGACTAATCTTCCTCAAGTCATAAAACAAAAG
  CTGACACCTTTATATGAGCTGGTAAAGGAAGTACCTTGTGCCTCTGTGAAAAAACTATACCTGAAATGGG
  CTCTTGAAGAGTATCTGGATGAATTTGACCCCTGTCATTGCCGGCCTTGTCAAAATGGTGGTTTGGCTAC
  TGTTGAGGGGACCCATTGTCTGTGCCATTGCAAACCGTACACATTTGGTGCGGCGTGTGAGCAAGGAGTC
  CTCGTAGGGAATCAAGCAGGAGGGGTTGATGGAGGTTGGAGTTGCTGGTCCTCTTGGAGCCCCTGTGTCC
  AAGGGAAGAAAACAAGAAGCCGTGAATGCAATAACCCACCTCCCAGTGGGGGTGGGAGATCCTGCGTTGG
  AGAAACGACAGAAAGCACACAATGCGAAGATGAGGAGCTGGAGCACTTGAGGTTGCTTGAACCACATTGC
  TTTCCTTTGTCTTTGGTTCCAACAGAATTCTGTCCATCACCTCCTGCCTTGAAAGATGGATTTGTTCAAG
  ATGAAGGTACAATGTTTCCTGTGGGGAAAAATGTAGTGTACACTTGCAATGAAGGATACTCTCTTATTGG
  AAACCCAGTGGCCAGATGTGGAGAAGATTTACGGTGGCTTGTTGGGGAAATGCATTGTCAGAAAATTGCC
  TGTGTTCTACCTGTACTGATGGATGGCATACAGAGTCACCCCCAAAAACCTTTCTACACAGTTGGTGAGA
  AGGTGACTGTTTCCTGTTCAGGTGGCATGTCCTTAGAAGGTCCTTCAGCATTTCTCTGTGGCTCCAGCCT
  TAAGTGGAGTCCTGAGATGAAGAATGCCCGCTGTGTACAAAAAGAAAATCCGTTAACACAGGCAGTGCCT
  AAATGTCAGCGCTGGGAGAAACTGCAGAATTCAAGATGTGTTTGTAAAATGCCCTACGAATGTGGACCTT
  CCTTGGATGTATGTGCTCAAGATGAGAGAAGCAAAAGGATACTGCCTCTGACAGTTTGCAAGATGCATGT
  TCTCCACTGTCAGGGTAGAAATTACACCCTTACTGGTAGGGACAGCTGTACTCTGCCTGCCTCAGCTGAG
  AAAGCTTGTGGTGCCTGCCCACTGTGGGGAAAATGTGATGCTGAGAGCAGCAAATGTGTCTGCCGAGAAG
  CATCGGAGTGCGAGGAAGAAGGGTTTAGCATTTGTGTGGAAGTGAACGGCAAGGAGCAGACGATGTCTGA
  GTGTGAGGCGGGCGCTCTGAGATGCAGAGGGCAGAGCATCTCTGTCACCAGCATAAGGCCTTGTGCTGCG
  GAAACCCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209562 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MKVISLFILVGFIGEFQSFSSASSPVNCQWDFYAPWSECNGCTKTQTRRRSVAVYGQYGGQPCVGNAFET
  QSCEPTRGCPTEEGCGERFRCFSGQCISKSLVCNGDSDCDEDSADEDRCEDSERRPSCDIDKPPPNIELT
  GNGYNELTGQFRNRVINTKSFGGQCRKVFSGDGKDFYRLSGNVLSYTFQVKINNDFNYEFYNSTWSYVKH
  TSTEHTSSSRKRSFFRSSSSSSRSYTSHTNEIHKGKSYQLLVVENTVEVAQFINNNPEFLQLAEPFWKEL
  SHLPSLYDYSAYRRLIDQYGTHYLQSGSLGGEYRVLFYVDSEKLKQNDFNSVEEKKCKSSGWHFVVKFSS
  HGCKELENALKAASGTQNNVLRGEPFIRGGGAGFISGLSYLELDNPAGNKRRYSAWAESVTNLPQVIKQK
  LTPLYELVKEVPCASVKKLYLKWALEEYLDEFDPCHCRPCQNGGLATVEGTHCLCHCKPYTFGAACEQGV
  LVGNQAGGVDGGWSCWSSWSPCVQGKKTRSRECNNPPPSGGGRSCVGETTESTQCEDEELEHLRLLEPHC
  FPLSLVPTEFCPSPPALKDGFVQDEGTMFPVGKNVVYTCNEGYSLIGNPVARCGEDLRWLVGEMHCQKIA
  CVLPVLMDGIQSHPQKPFYTVGEKVTVSCSGGMSLEGPSAFLCGSSLKWSPEMKNARCVQKENPLTQAVP
  KCQRWEKLQNSRCVCKMPYECGPSLDVCAQDERSKRILPLTVCKMHVLHCQGRNYTLTGRDSCTLPASAE
  KACGACPLWGKCDAESSKCVCREASECEEEGFSICVEVNGKEQTMSECEAGALRCRGQSISVTSIRPCAA
  ETQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000587
• ORF Size: 2529 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000587.4
• RefSeq Size: 4034 bp
• RefSeq ORF: 2532 bp
• Locus ID: 730
• UniProt ID: P10643
• Cytogenetics: 5p13.1
• Domains: CCP, tsp_1, MACPF, ldl_recept_a, FIMAC
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Complement and coagulation cascades, Prion diseases, Systemic lupus erythematosus
• MW: 93.5 kDa
• Gene Summary: This gene encodes a serum glycoprotein that forms a membrane attack complex together with complement components C5b, C6, C8, and C9 as part of the terminal complement pathway of the innate immune system. The protein encoded by this gene contains a cholesterol-dependent cytolysin/membrane attack complex/perforin-like (CDC/MACPF) domain and belongs to a large family of structurally related molecules that form pores involved in host immunity and bacterial pathogenesis. This protein initiates membrane attack complex formation by binding the C5b-C6 subcomplex and inserts into the phospholipid bilayer, serving as a membrane anchor. Mutations in this gene are associated with a rare disorder called C7 deficiency. [provided by RefSeq, Nov 2016]