OSBPL11 (NM_022776) Human Tagged ORF Clone

CAT#: RC209418

OSBPL11 (Myc-DDK-tagged)-Human oxysterol binding protein-like 11 (OSBPL11)

Product Images

Circular map for RC209418

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY OSBPL11 (Cat# RC209418, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-OSBPL11(Cat# TA502068). Positive lysates LY411565 (100ug) and LC411565 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY411565) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC209418 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified OSBPL11 protein (Cat# TP309418). The protein was produced from HEK293T cells transfected with OSBPL11 cDNA clone (Cat# RC209418) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: OSBPL11
• Synonyms: ORP-11; ORP11; OSBP12; TCCCIA00292
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209418 representing NM_022776
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGGGGGGTGAACCAGTGTCCACAATGAAAGTCTCGGAGAGCGAAGGAAAGCTGGAGGGCCAGGCCA
  CAGCGGTGACCCCGAACAAGAACAGCAGCTGTGGAGGTGGAATCAGTAGCAGCAGCAGCAGCCGCGGTGG
  CAGTGCAAAAGGCTGGCAGTACAGTGATCACATGGAAAATGTGTATGGCTATTTAATGAAGTATACCAAC
  CTTGTCACTGGGTGGCAGTACAGGTTTTTTGTTTTAAACAATGAAGCTGGGCTGTTGGAGTACTTTGTGA
  ATGAACAGTCTAGAAATCAGAAACCTAGAGGAACTTTGCAGCTTGCAGGAGCTGTAATATCACCCAGTGA
  TGAGGATTCTCACACCTTCACTGTAAACGCTGCCAGTGGGGAACAATATAAACTCAGAGCTACAGATGCA
  AAAGAGCGACAGCACTGGGTTAGCAGACTTCAGATATGTACACAGCATCATACTGAAGCTATTGGAAAGA
  ATAATCCTCCTCTGAAGTCACGGAGCTTCTCACTTGCATCTAGTAGTAATTCTCCTATATCGCAGAGGAG
  ACCAAGTCAAAATGCCATTTCTTTTTTTAATGTTGGACATTCCAAACTGCAATCACTGAGCAAAAGAACT
  AATTTACCTCCAGACCATCTTGTGGAAGTCAGAGAAATGATGTCTCATGCTGAAGGACAACAAAGAGACT
  TAATTAGACGAATTGAATGCCTTCCTACTTCTGGCCATCTTAGTTCCTTGGACCAGGATCTCTTAATGCT
  CAAAGCTACTTCCATGGCAACTATGAACTGCTTAAATGACTGCTTTCATATTCTCCAGTTACAGCATGCA
  TCACATCAGAAGGGCTCATTGCCTTCAGGAACGACAATCGAGTGGTTAGAACCAAAGATATCTTTATCAA
  ACCACTATAAAAATGGAGCTGACCAGCCCTTTGCAACTGATCAGAGTAAGCCGGTGGCAGTCCCAGAAGA
  GCAGCCTGTTGCAGAATCTGGACTATTAGCGAGGGAGCCTGAAGAAATAAATGCAGATGATGAGATAGAG
  GATACATGTGACCACAAAGAGGATGACCTGGGAGCTGTAGAAGAACAACGTAGTGTCATCCTACATCTCT
  TGTCACAGCTTAAGCTGGGCATGGATTTAACAAGAGTGGTGCTTCCTACATTTATCCTAGAGAAGCGTTC
  CTTGCTGGAAATGTATGCAGACTTTATGTCTCATCCAGACCTATTTATAGCCATCACTAATGGAGCCACA
  GCTGAGGACAGAATGATTCGCTTTGTTGAGTACTACCTTACCTCATTTCATGAAGGCCGTAAGGGAGCCA
  TTGCTAAAAAACCATACAATCCTATCATTGGAGAAACATTTCACTGTTCCTGGAAGATGCCAAAAAGCGA
  GGTAGCATCCAGTGTTTTTAGCAGTTCTTCCACCCAGGGAGTCACAAATCATGCTCCTTTATCGGGGGAG
  TCTTTGACCCAGGTGGGATCAGACTGTTACACAGTCAGATTTGTTGCTGAGCAGGTTTCTCATCATCCTC
  CAGTCTCAGGATTTTATGCAGAATGTACAGAGAGGAAGATGTGTGTAAATGCGCATGTCTGGACTAAGAG
  CAAGTTCTTAGGCATGTCAATAGGCGTGACAATGGTTGGAGAAGGTATCCTTAGTCTGTTGGAGCATGGA
  GAAGAGTACACATTTTCTCTACCCTGTGCATATGCTCGGTCAATTTTGACTGTTCCTTGGGTAGAACTGG
  GTGGCAAAGTCAGTGTCAACTGTGCAAAAACTGGATATTCAGCCAGCATCACTTTTCATACCAAGCCATT
  TTATGGTGGCAAACTGCATCGGGTTACAGCTGAAGTAAAGCACAACATCACCAACACTGTGGTATGCAGA
  GTGCAAGGGGAATGGAATAGTGTTCTTGAGTTCACATATAGCAATGGAGAGACAAAGTATGTGGACTTGA
  CTAAATTGGCAGTGACGAAGAAAAGAGTGAGACCTCTGGAGAAGCAGGATCCATTTGAATCCAGGCGATT
  GTGGAAAAATGTGACAGACTCGCTGAGAGAATCTGAAATTGATAAGGCCACAGAGCATAAGCATACCCTG
  GAAGAACGTCAGAGGACTGAAGAAAGGCATCGTACTGAAACAGGCACACCTTGGAAAACCAAATATTTTA
  TTAAAGAGGGAGATGGCTGGGTTTATCATAAACCACTTTGGAAAATAATTCCAACAACACAACCAGCAGA
  G
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209418 representing NM_022776
  Red=Cloning site Green=Tags(s)
  
  MQGGEPVSTMKVSESEGKLEGQATAVTPNKNSSCGGGISSSSSSRGGSAKGWQYSDHMENVYGYLMKYTN
  LVTGWQYRFFVLNNEAGLLEYFVNEQSRNQKPRGTLQLAGAVISPSDEDSHTFTVNAASGEQYKLRATDA
  KERQHWVSRLQICTQHHTEAIGKNNPPLKSRSFSLASSSNSPISQRRPSQNAISFFNVGHSKLQSLSKRT
  NLPPDHLVEVREMMSHAEGQQRDLIRRIECLPTSGHLSSLDQDLLMLKATSMATMNCLNDCFHILQLQHA
  SHQKGSLPSGTTIEWLEPKISLSNHYKNGADQPFATDQSKPVAVPEEQPVAESGLLAREPEEINADDEIE
  DTCDHKEDDLGAVEEQRSVILHLLSQLKLGMDLTRVVLPTFILEKRSLLEMYADFMSHPDLFIAITNGAT
  AEDRMIRFVEYYLTSFHEGRKGAIAKKPYNPIIGETFHCSWKMPKSEVASSVFSSSSTQGVTNHAPLSGE
  SLTQVGSDCYTVRFVAEQVSHHPPVSGFYAECTERKMCVNAHVWTKSKFLGMSIGVTMVGEGILSLLEHG
  EEYTFSLPCAYARSILTVPWVELGGKVSVNCAKTGYSASITFHTKPFYGGKLHRVTAEVKHNITNTVVCR
  VQGEWNSVLEFTYSNGETKYVDLTKLAVTKKRVRPLEKQDPFESRRLWKNVTDSLRESEIDKATEHKHTL
  EERQRTEERHRTETGTPWKTKYFIKEGDGWVYHKPLWKIIPTTQPAE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022776
• ORF Size: 2241 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022776.5
• RefSeq Size: 4206 bp
• RefSeq ORF: 2244 bp
• Locus ID: 114885
• UniProt ID: Q9BXB4
• Cytogenetics: 3q21.2
• Domains: Oxysterol_BP, PH
• MW: 83.5 kDa
• Gene Summary: This gene encodes a member of the oxysterol-binding protein (OSBP) family, a group of intracellular lipid receptors. Like most members, the encoded protein contains an N-terminal pleckstrin homology domain and a highly conserved C-terminal OSBP-like sterol-binding domain. [provided by RefSeq, Jul 2008]