SnoN (SKIL) (NM_005414) Human Tagged ORF Clone

CAT#: RC209251

SKIL (Myc-DDK-tagged)-Human SKI-like oncogene (SKIL), transcript variant 1

Product Images

Circular map for RC209251

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY SKIL (Cat# RC209251, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SKIL(Cat# TA800171). Positive lysates LY417307 (100ug) and LC417307 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY417307) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC209251 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SnoN
• Synonyms: SNO; SnoA; SnoI; SnoN
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209251 representing NM_005414
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAAACCTCCAGACAAATTTCTCCTTGGTTCAGGGCTCAACTAAAAAACTGAATGGGATGGGAGATG
  ATGGCAGCCCCCCAGCGAAAAAAATGATAACGGACATTCATGTAAATGGAAAAACGATAAACAAGGTGCC
  AACAGTTAAGAAGGAACACTTGGATGACTATGGAGAAGCACCAGTGGAAACTGATGGAGAGCATGTTAAG
  CGAACCTGTACTTCTGTTCCTGAAACTTTGCATTTAAATCCCAGTTTGAAACACACATTGGCACAATTCC
  ATTTAAGTAGTCAGAGCTCGCTGGGTGGACCAGCAGCATTTTCTGCTCGGCATTCCCAAGAAAGCATGTC
  GCCTACTGTATTTCTGCCTCTTCCATCACCTCAGGTTCTTCCTGGCCCATTGCTCATCCCTTCAGATAGC
  TCCACAGAACTCACTCAGACTGTGTTGGAAGGGGAATCTATTTCTTGTTTTCAAGTTGGAGGAGAAAAGA
  GACTCTGTTTGCCCCAAGTCTTAAATTCTGTTCTCCGAGAATTTACACTCCAGCAAATAAATACAGTGTG
  TGATGAACTGTACATATATTGTTCAAGGTGTACTTCAGACCAGCTTCATATCTTAAAGGTACTGGGCATA
  CTTCCATTCAATGCCCCATCCTGTGGGCTGATTACATTAACTGATGCACAAAGATTATGTAATGCTTTAT
  TGCGGCCACGAACTTTTCCTCAAAATGGTAGCGTACTTCCTGCTAAAAGCTCATTGGCCCAGTTAAAGGA
  AACTGGCAGTGCCTTTGAAGTTGAGCATGAATGCCTAGGCAAATGTCAGGGTTTATTTGCACCCCAGTTT
  TATGTTCAGCCTGATGCTCCGTGTATTCAATGTCTGGAGTGTTGTGGAATGTTTGCACCCCAGACGTTTG
  TGATGCATTCTCACAGATCACCTGACAAAAGAACTTGCCACTGGGGCTTTGAATCAGCTAAATGGCATTG
  CTATCTTCATGTGAACCAAAAATACTTAGGAACACCTGAAGAAAAGAAACTGAAGATAATTTTAGAAGAA
  ATGAAGGAGAAGTTTAGCATGAGAAGTGGAAAGAGAAATCAATCCAAGACAGATGCACCATCAGGAATGG
  AATTACAGTCATGGTATCCTGTTATAAAGCAGGAAGGTGACCATGTTTCTCAGACACATTCATTTTTACA
  CCCCAGCTACTACTTATACATGTGTGATAAAGTGGTTGCCCCAAATGTGTCACTTACTTCTGCTGTATCC
  CAGTCTAAAGAGCTCACAAAGACAGAGGCAAGTAAGTCCATATCAAGACAGTCAGAGAAGGCTCACAGTA
  GTGGTAAACTTCAAAAAACAGTGTCTTATCCAGATGTCTCACTTGAGGAACAGGAGAAAATGGATTTAAA
  AACAAGTAGAGAATTATGTAGCCGTTTAGATGCATCAATCTCAAATAATTCTACAAGTAAAAGGAAATCT
  GAGTCTGCCACTTGCAACTTAGTCAGAGACATAAACAAAGTGGGAATTGGCCTTGTTGCTGCCGCTTCAT
  CTCCGCTTCTTGTGAAAGATGTCATTTGTGAGGATGATAAGGGAAAAATCATGGAAGAAGTAATGAGAAC
  TTATTTAAAACAACAGGAAAAACTAAACTTGATTTTGCAAAAGAAGCAACAACTTCAGATGGAAGTAAAA
  ATGTTGAGTAGTTCAAAATCTATGAAGGAACTCACTGAAGAACAGCAGAATTTACAGAAAGAGCTTGAAT
  CTTTGCAGAATGAACATGCTCAAAGAATGGAAGAATTTTATGTTGAACAGAAAGACTTAGAGAAAAAATT
  GGAGCAGATAATGAAGCAAAAATGTACCTGTGACTCAAATTTAGAAAAAGACAAAGAGGCTGAATATGCA
  GGACAGTTGGCAGAACTGAGGCAGAGATTGGACCATGCTGAGGCCGATAGGCAAGAACTCCAAGATGAAC
  TCAGACAGGAACGGGAAGCAAGACAGAAGTTAGAGATGATGATAAAAGAGCTAAAGCTGCAAATTCTGAA
  ATCATCAAAGACTGCTAAAGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209251 representing NM_005414
  Red=Cloning site Green=Tags(s)
  
  MENLQTNFSLVQGSTKKLNGMGDDGSPPAKKMITDIHVNGKTINKVPTVKKEHLDDYGEAPVETDGEHVK
  RTCTSVPETLHLNPSLKHTLAQFHLSSQSSLGGPAAFSARHSQESMSPTVFLPLPSPQVLPGPLLIPSDS
  STELTQTVLEGESISCFQVGGEKRLCLPQVLNSVLREFTLQQINTVCDELYIYCSRCTSDQLHILKVLGI
  LPFNAPSCGLITLTDAQRLCNALLRPRTFPQNGSVLPAKSSLAQLKETGSAFEVEHECLGKCQGLFAPQF
  YVQPDAPCIQCLECCGMFAPQTFVMHSHRSPDKRTCHWGFESAKWHCYLHVNQKYLGTPEEKKLKIILEE
  MKEKFSMRSGKRNQSKTDAPSGMELQSWYPVIKQEGDHVSQTHSFLHPSYYLYMCDKVVAPNVSLTSAVS
  QSKELTKTEASKSISRQSEKAHSSGKLQKTVSYPDVSLEEQEKMDLKTSRELCSRLDASISNNSTSKRKS
  ESATCNLVRDINKVGIGLVAAASSPLLVKDVICEDDKGKIMEEVMRTYLKQQEKLNLILQKKQQLQMEVK
  MLSSSKSMKELTEEQQNLQKELESLQNEHAQRMEEFYVEQKDLEKKLEQIMKQKCTCDSNLEKDKEAEYA
  GQLAELRQRLDHAEADRQELQDELRQEREARQKLEMMIKELKLQILKSSKTAKE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005414
• ORF Size: 2052 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005414.2, NP_005405.1
• RefSeq Size: 3111 bp
• RefSeq ORF: 2055 bp
• Locus ID: 6498
• UniProt ID: P12757
• Cytogenetics: 3q26.2
• Domains: Ski_Sno
• Protein Families: Druggable Genome, Transcription Factors
• MW: 76.8 kDa
• Gene Summary: The protein encoded by this gene is a component of the SMAD pathway, which regulates cell growth and differentiation through transforming growth factor-beta (TGFB). In the absence of ligand, the encoded protein binds to the promoter region of TGFB-responsive genes and recruits a nuclear repressor complex. TGFB signaling causes SMAD3 to enter the nucleus and degrade this protein, allowing these genes to be activated. Four transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]