CNNM4 (NM_020184) Human Tagged ORF Clone

CAT#: RC209231

CNNM4 (Myc-DDK-tagged)-Human cyclin M4 (CNNM4)

Product Images

Circular map for RC209231

• 

  Western blot validation of overexpression lysate (Cat# LY412607) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC209231 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CNNM4
• Synonyms: ACDP4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209231 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCCGGTGGGCGGGGGCGGGCGCCCGGTCGGCGGACCGGCCCGCGGGCGCCTCCTCCTGGCGGCGC
  CGGTGCTGCTGGTGCTGCTGTGGGCGCTGGGGGCCCGGGGCCAGGGCAGCCCCCAGCAGGGCACGATCGT
  GGGCATGAGGCTGGCGAGCTGCAACAAGTCGTGTGGGACGAACCCGGATGGCATCATCTTCGTGTCCGAG
  GGCAGCACCGTGAACCTGAGGCTGTACGGCTACAGCCTGGGCAACATCTCCAGCAACCTGATCTCCTTCA
  CCGAGGTGGACGATGCCGAGACCCTCCACAAGTCCACCAGCTGCCTCGAGCTCACCAAGGACCTGGTCGT
  CCAGCAGCTGGTCAACGTGAGCCGCAGGAACACGTCCGGCGTGCTGGTGGTGCTCACCAAGTTCCTCCGG
  AGGAGCGAGAGCATGAAGCTGTATGCACTGTGCACCCGGGCCCAGCCCGACGGGCCCTGGCTGAAGTGGA
  CGGACAAGGACTCACTGCTCTTCATGGTGGAGGAGCCTGGGAGGTTCCTGCCTCTCTGGCTGCACATTCT
  CCTAATTACGGTGCTGCTGGTGCTGTCGGGCATATTTTCTGGCCTCAACCTCGGGCTTATGGCCCTGGAC
  CCCATGGAGCTGCGCATCGTGCAGAACTGTGGCACCGAGAAGGAGAGGCGCTATGCCCGCAAGATTGAGC
  CCATCCGGCGCAAGGGCAACTACCTTCTCTGCTCGTTGCTCCTAGGGAACGTGCTGGTCAACACCTCCCT
  CACAATCCTTCTAGACAACCTCATCGGGTCCGGCCTCATGGCGGTGGCCTCCTCCACCATTGGCATTGTC
  ATCTTTGGGGAGATCCTACCTCAGGCCCTGTGCTCCCGACATGGGCTGGCTGTGGGTGCCAACACCATCC
  TTCTCACCAAATTCTTTATGCTACTCACCTTCCCCCTCAGTTTTCCCATTAGCAAGCTCCTGGACTTTTT
  TCTGGGCCAGGAGATTCGCACTGTTTACAACCGGGAGAAGCTGATGGAGATGTTGAAGGTGACGGAGCCC
  TATAATGACCTCGTGAAAGAGGAGCTCAATATGATCCAGGGTGCCCTGGAACTACGGACCAAAACTGTAG
  AGGATATCATGACCCAGCTCCAGGACTGCTTCATGATCCGCAGCGATGCCATCCTGGACTTCAACACCAT
  GTCGGAGATAATGGAAAGCGGCTATACTCGCATCCCGGTGTTCGAAGACGAGCAGTCCAATATTGTAGAT
  ATTCTCTACGTCAAAGACTTGGCCTTTGTGGACCCCGATGACTGCACCCCCCTCAAGACTATCACTCGCT
  TCTATAACCACCCGGTGCACTTTGTCTTCCATGACACCAAGTTGGATGCCATGCTGGAGGAGTTCAAGAA
  GGGGAAGTCCCACCTGGCCATCGTGCAGAAGGTAAACAACGAGGGTGAGGGTGACCCCTTCTACGAGGTC
  CTGGGCCTGGTCACCCTGGAGGACGTGATCGAGGAGATCATCAAGTCGGAGATCCTGGACGAGTCCGACA
  TGTACACTGACAACCGAAGCCGGAAGCGGGTGTCTGAGAAGAACAAGCGTGACTTCTCTGCCTTCAAGGA
  TGCGGACAATGAGCTCAAAGTGAAAATCTCCCCGCAGCTCCTCCTGGCCGCTCATCGCTTCCTAGCCACA
  GAGGTCTCTCAGTTTAGCCCCTCCCTGATATCAGAGAAGATCCTGCTGCGGCTACTCAAGTACCCAGATG
  TCATTCAGGAACTCAAGTTTGACGAGCACAATAAGTACTACGCCCGCCATTACCTGTACACCCGAAATAA
  GCCGGCCGACTACTTCATCCTCATCCTGCAGGGGAAGGTGGAGGTGGAGGCAGGGAAGGAGAACATGAAG
  TTTGAGACGGGCGCCTTCTCCTACTATGGGACTATGGCCCTGACCTCGGTCCCCTCCGACCGTTCCCCAG
  CACACCCCACCCCACTCAGCCGCTCAGCCTCCCTCAGTTACCCAGACCGCACAGACGTCTCAACTGCAGC
  AACCTTGGCAGGCAGCAGCAACCAGTTTGGCAGCTCTGTCCTGGGCCAGTACATCTCTGACTTCAGCGTC
  CGGGCACTCGTGGACTTGCAGTACATCAAGATCACTCGGCAGCAGTACCAGAACGGGCTGCTGGCTTCTC
  GCATGGAGAACAGCCCTCAGTTTCCCATAGACGGGTGCACCACCCACATGGAGAACTTGGCCGAGAAGTC
  TGAGCTGCCTGTGGTGGACGAGACCACAACTCTTCTCAACGAGCGTAACTCCTTGCTGCACAAAGCCTCC
  CACGAGAATGCCATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209231 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAPVGGGGRPVGGPARGRLLLAAPVLLVLLWALGARGQGSPQQGTIVGMRLASCNKSCGTNPDGIIFVSE
  GSTVNLRLYGYSLGNISSNLISFTEVDDAETLHKSTSCLELTKDLVVQQLVNVSRRNTSGVLVVLTKFLR
  RSESMKLYALCTRAQPDGPWLKWTDKDSLLFMVEEPGRFLPLWLHILLITVLLVLSGIFSGLNLGLMALD
  PMELRIVQNCGTEKERRYARKIEPIRRKGNYLLCSLLLGNVLVNTSLTILLDNLIGSGLMAVASSTIGIV
  IFGEILPQALCSRHGLAVGANTILLTKFFMLLTFPLSFPISKLLDFFLGQEIRTVYNREKLMEMLKVTEP
  YNDLVKEELNMIQGALELRTKTVEDIMTQLQDCFMIRSDAILDFNTMSEIMESGYTRIPVFEDEQSNIVD
  ILYVKDLAFVDPDDCTPLKTITRFYNHPVHFVFHDTKLDAMLEEFKKGKSHLAIVQKVNNEGEGDPFYEV
  LGLVTLEDVIEEIIKSEILDESDMYTDNRSRKRVSEKNKRDFSAFKDADNELKVKISPQLLLAAHRFLAT
  EVSQFSPSLISEKILLRLLKYPDVIQELKFDEHNKYYARHYLYTRNKPADYFILILQGKVEVEAGKENMK
  FETGAFSYYGTMALTSVPSDRSPAHPTPLSRSASLSYPDRTDVSTAATLAGSSNQFGSSVLGQYISDFSV
  RALVDLQYIKITRQQYQNGLLASRMENSPQFPIDGCTTHMENLAEKSELPVVDETTTLLNERNSLLHKAS
  HENAI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020184
• ORF Size: 2325 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020184.3, NP_064569.3
• RefSeq Size: 4814 bp
• RefSeq ORF: 2328 bp
• Locus ID: 26504
• UniProt ID: Q6P4Q7
• Cytogenetics: 2q11.2
• Domains: CBS, DUF21
• Protein Families: Transmembrane
• MW: 86.7 kDa
• Gene Summary: This gene encodes a member of the ancient conserved domain containing protein family. Members of this protein family contain a cyclin box motif and have structural similarity to the cyclins. The encoded protein may play a role in metal ion transport. Mutations in this gene are associated with Jalili syndrome which consists of cone-rod dystrophy and amelogenesis imperfecta. [provided by RefSeq, Feb 2010]