IP3KC (ITPKC) (NM_025194) Human Tagged ORF Clone

CAT#: RC209200

ITPKC (Myc-DDK-tagged)-Human inositol-trisphosphate 3-kinase C (ITPKC)

Product Images

Circular map for RC209200

• 

  Western blot validation of overexpression lysate (Cat# LY410845) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC209200 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified ITPKC protein (Cat# TP309200). The protein was produced from HEK293T cells transfected with ITPKC cDNA clone (Cat# RC209200) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: IP3KC
• Synonyms: IP3-3KC; IP3KC
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209200 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGCGCTGCCCGTGCCGTGGGAGCCTGAACGAGGCGGAGGCCGGGGCGCTGCCCGCGGCGGCCCGCA
  TGGGACTGGAGGCGCCGCGAGGAGGGCGGCGGCGGCAGCCGGGACAGCAGCGACCTGGGCCCGGCGCAGG
  GGCCCCGGCGGGGCGGCCGGAGGGGGGCGGGCCCTGGGCCCGGACAGAGGGGTCCAGCCTCCACAGCGAG
  CCTGAGAGGGCCGGCCTCGGGCCTGCGCCGGGGACAGAGAGTCCGCAGGCAGAATTCTGGACAGACGGAC
  AGACTGAGCCCGCGGCAGCTGGCCTTGGAGTAGAGACCGAGAGGCCCAAGCAAAAGACGGAGCCAGACAG
  GTCCAGCCTCCGGACGCATCTAGAATGGAGCTGGTCAGAGCTGGAGACGACTTGTCTTTGGACGGAGACC
  GGGACAGATGGCCTTTGGACTGATCCGCACAGGTCCGACCTCCAGTTTCAGCCCGAGGAGGCCAGCCCCT
  GGACACAGCCAGGGGTTCATGGGCCCTGGACAGAGCTGGAAACGCATGGGTCACAGACTCAGCCAGAGAG
  GGTCAAGTCCTGGGCTGATAACCTCTGGACCCACCAGAACAGTTCCAGCCTCCAGACTCACCCAGAAGGA
  GCCTGTCCCTCAAAAGAGCCAAGTGCTGATGGCTCCTGGAAAGAATTGTATACTGATGGCTCCAGGACAC
  AACAGGATATTGAAGGTCCCTGGACAGAGCCATATACTGATGGCTCCCAGAAAAAACAGGATACTGAAGC
  AGCCAGGAAACAGCCTGGCACTGGTGGTTTCCAAATACAACAGGATACTGATGGCTCCTGGACACAACCT
  AGCACTGACGGTTCCCAGACAGCACCTGGGACAGACTGCCTCTTGGGAGAGCCTGAGGATGGCCCATTAG
  AGGAACCAGAGCCTGGAGAATTGCTGACTCACCTGTACTCTCACCTGAAGTGTAGCCCCCTGTGCCCTGT
  GCCCCGCCTCATCATTACCCCTGAGACCCCTGAGCCTGAGGCCCAGCCAGTGGGACCCCCCTCCCGGGTT
  GAGGGGGGCAGCGGCGGCTTCTCCTCTGCCTCTTCTTTCGACGAGTCTGAGGATGACGTGGTGGCCGGGG
  GCGGAGGTGCCAGCGATCCCGAGGACAGGTCTGGGAGCAAACCCTGGAAGAAGCTGAAGACAGTTCTGAA
  GTATTCACCCTTTGTGGTCTCCTTCCGAAAACACTACCCTTGGGTCCAGCTTTCTGGACATGCTGGGAAC
  TTCCAGGCAGGAGAGGATGGTCGGATTCTGAAACGTTTCTGTCAGTGTGAGCAGCGCAGCCTGGAGCAGC
  TGATGAAAGACCCGCTGCGACCTTTCGTGCCTGCCTACTATGGCATGGTGCTGCAGGATGGCCAGACCTT
  CAACCAGATGGAAGACCTCCTGGCTGACTTTGAGGGCCCCTCCATTATGGACTGCAAGATGGGCAGCAGG
  ACCTATCTGGAAGAGGAGCTAGTGAAGGCACGGGAACGTCCCCGTCCCCGGAAGGACATGTATGAGAAGA
  TGGTGGCTGTGGACCCTGGGGCCCCTACCCCTGAGGAGCATGCCCAGGGTGCAGTCACCAAGCCCCGCTA
  CATGCAGTGGAGGGAAACCATGAGCTCCACCTCTACCCTGGGCTTCCGGATCGAGGGCATCAAGAAGGCA
  GATGGGACCTGTAACACCAACTTCAAGAAGACGCAGGCACTGGAGCAGGTGACAAAAGTGCTGGAGGACT
  TCGTGGATGGAGACCACGTCATCCTGCAAAAGTACGTGGCATGCCTAGAAGAACTTCGTGAAGCTCTGGA
  GATCTCCCCCTTCTTCAAGACCCACGAGGTGGTAGGCAGCTCCCTCCTCTTCGTGCACGACCACACCGGC
  CTGGCCAAGGTCTGGATGATAGACTTCGGCAAGACGGTGGCCTTGCCCGACCACCAGACGCTCAGCCACA
  GGCTGCCCTGGGCTGAGGGCAACCGTGAGGACGGCTACCTCTGGGGCCTGGACAACATGATCTGCCTCCT
  GCAGGGGCTGGCACAGAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209200 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MRRCPCRGSLNEAEAGALPAAARMGLEAPRGGRRRQPGQQRPGPGAGAPAGRPEGGGPWARTEGSSLHSE
  PERAGLGPAPGTESPQAEFWTDGQTEPAAAGLGVETERPKQKTEPDRSSLRTHLEWSWSELETTCLWTET
  GTDGLWTDPHRSDLQFQPEEASPWTQPGVHGPWTELETHGSQTQPERVKSWADNLWTHQNSSSLQTHPEG
  ACPSKEPSADGSWKELYTDGSRTQQDIEGPWTEPYTDGSQKKQDTEAARKQPGTGGFQIQQDTDGSWTQP
  STDGSQTAPGTDCLLGEPEDGPLEEPEPGELLTHLYSHLKCSPLCPVPRLIITPETPEPEAQPVGPPSRV
  EGGSGGFSSASSFDESEDDVVAGGGGASDPEDRSGSKPWKKLKTVLKYSPFVVSFRKHYPWVQLSGHAGN
  FQAGEDGRILKRFCQCEQRSLEQLMKDPLRPFVPAYYGMVLQDGQTFNQMEDLLADFEGPSIMDCKMGSR
  TYLEEELVKARERPRPRKDMYEKMVAVDPGAPTPEEHAQGAVTKPRYMQWRETMSSTSTLGFRIEGIKKA
  DGTCNTNFKKTQALEQVTKVLEDFVDGDHVILQKYVACLEELREALEISPFFKTHEVVGSSLLFVHDHTG
  LAKVWMIDFGKTVALPDHQTLSHRLPWAEGNREDGYLWGLDNMICLLQGLAQS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_025194
• ORF Size: 2049 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025194.3
• RefSeq Size: 3398 bp
• RefSeq ORF: 2052 bp
• Locus ID: 80271
• UniProt ID: Q96DU7
• Cytogenetics: 19q13.2
• Domains: IPK
• Protein Families: Druggable Genome
• MW: 75.2 kDa
• Gene Summary: This gene encodes a member of the inositol 1,4,5-trisphosphate [Ins(1,4,5)P(3)] 3-kinase family of enzymes that catalyze the phosphorylation of inositol 1,4,5-trisphosphate to 1,3,4,5-tetrakisphosphate. The encoded protein is localized to the nucleus and cytoplasm and has both nuclear import and nuclear export activity. Single nucleotide polymorphisms in this gene are associated with Kawasaki disease.[provided by RefSeq, Sep 2009]