ADAMTS18 (NM_199355) Human Tagged ORF Clone

CAT#: RC209192

ADAMTS18 (Myc-DDK-tagged)-Human ADAM metallopeptidase with thrombospondin type 1 motif, 18 (ADAMTS18)

Product Images

Circular map for RC209192

• 

  Western blot validation of overexpression lysate (Cat# LY404538) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC209192 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ADAMTS18
• Synonyms: ADAMTS21; KNO2; MMCAT
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209192 representing NM_199355
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGTGCGCCCTCCTGCTCGCGTGTGCCTTCCCGGCTGCGGGTTCGGGCCCGCCGAGGGGCCTGGCGG
  GACTGGGGCGCGTGGCCAAGGCGCTCCAGCTGTGCTGCCTCTGCTGTGCGTCGGTCGCCGCGGCCTTAGC
  CAGTGACAGCAGCAGCGGCGCCAGCGGATTAAATGATGATTACGTCTTTGTCACGCCAGTAGAAGTAGAC
  TCAGCCGGGTCATATATTTCACACGACATTTTGCACAACGGCAGGAAAAAGCGATCGGCGCAGAATGCCA
  GAAGCTCCCTGCACTACCGATTTTCAGCATTTGGACAGGAACTGCACTTAGAACTTAAGCCCTCGGCGAT
  TTTGAGCAGTCACTTTATTGTCCAGGTACTTGGAAAAGATGGTGCTTCAGAGACTCAGAAACCCGAGGTG
  CAGCAATGCTTCTATCAGGGATTTATCAGAAATGACAGCTCCTCCTCTGTCGCTGTGTCTACGTGTGCTG
  GCTTGTCAGGTTTAATAAGGACACGAAAAAATGAATTCCTCATCTCGCCATTACCTCAGCTTCTGGCCCA
  GGAACACAACCACAGCTCCCCTGCGGGTCACCATCCTCACGTACTGTACAAAAGGACAGCAGAGGAGAAG
  ATCCAGCGGTACCGTGGCTACCCCGGCTCTGGCCGGAATTATCCTGGTTACTCCCCAAGTCACATTCCCC
  ATGCATCTCAGAGTCGAGAGACAGAGTATCACCATCGAAGGTTGCAAAAGCAGCATTTTTGTGGACGACG
  CAAGAAATATGCTCCCAAGCCTCCCACAGAGGACACCTATCTAAGGTTTGATGAATATGGGAGCTCTGGG
  CGACCCAGAAGATCAGCTGGAAAATCACAAAAGGGCCTCAATGTGGAAACCCTCGTGGTGGCAGACAAGA
  AAATGGTGGAAAAGCATGGCAAGGGAAATGTCACCACATACATTCTCACAGTAATGAACATGGTTTCTGG
  CCTATTTAAAGATGGGACTATTGGAAGTGACATAAACGTGGTTGTGGTGAGCCTAATTCTTCTGGAACAA
  GAACCTGGAGGATTATTGATCAACCATCATGCAGACCAGTCTCTGAATAGTTTTTGTCAATGGCAGTCTG
  CCCTCATTGGAAAGAATGGCAAGAGACATGATCATGCCATCTTACTAACAGGATTTGATATTTGTTCTTG
  GAAGAATGAACCATGTGACACTCTAGGGTTTGCCCCCATCAGTGGAATGTGCTCTAAGTACCGAAGTTGT
  ACCATCAATGAGGACACAGGACTTGGCCTTGCCTTCACCATCGCTCATGAGTCAGGGCACAACTTTGGTA
  TGATTCACGACGGAGAAGGGAATCCCTGCAGAAAGGCTGAAGGCAATATCATGTCTCCCACACTGACCGG
  AAACAATGGAGTGTTTTCATGGTCTTCCTGCAGCCGCCAGTATCTCAAGAAATTCCTCAGCACACCTCAG
  GCGGGGTGTCTAGTGGATGAGCCCAAGCAAGCAGGACAGTATAAATATCCGGACAAACTACCAGGACAGA
  TTTATGATGCTGACACACAGTGTAAATGGCAATTTGGAGCAAAAGCCAAGTTATGCAGCCTTGGTTTTGT
  GAAGGATATTTGCAAATCACTTTGGTGCCACCGAGTAGGCCACAGGTGTGAGACCAAGTTTATGCCCGCA
  GCAGAAGGGACCGTTTGTGGCTTGAGTATGTGGTGTCGGCAAGGCCAGTGCGTAAAGTTTGGGGAGCTCG
  GGCCCCGGCCCATCCACGGCCAGTGGTCCGCCTGGTCGAAGTGGTCAGAATGTTCCCGGACATGTGGTGG
  AGGAGTCAAGTTCCAGGAGAGACACTGCAATAACCCCAAGCCTCAGTATGGTGGCATATTCTGTCCAGGT
  TCTAGCCGTATTTATCAGCTGTGCAATATTAACCCTTGCAATGAAAATAGCTTGGATTTTCGGGCTCAAC
  AGTGTGCAGAATATAACAGCAAACCTTTCCGTGGATGGTTCTACCAGTGGAAACCCTATACAAAAGTGGA
  AGAGGAAGATCGATGCAAACTGTACTGCAAGGCTGAGAACTTTGAATTTTTTTTTGCAATGTCCGGCAAA
  GTGAAAGATGGAACTCCCTGCTCCCCAAACAAAAATGATGTTTGTATTGACGGGGTTTGTGAACTAGTGG
  GATGTGATCATGAACTAGGCTCTAAAGCAGTTTCAGATGCTTGTGGCGTTTGCAAAGGTGATAATTCAAC
  TTGCAAGTTTTATAAAGGCCTGTACCTCAACCAGCATAAAGCAAATGAATATTATCCGGTGGTCATCATT
  CCAGCTGGCGCCCGAAGCATCGAAATCCAGGAGCTGCAGGTTTCCTCCAGTTACCTCGCAGTTCGAAGCC
  TCAGTCAAAAGTATTACCTCACCGGGGGCTGGAGCATCGACTGGCCTGGGGAGTTCCCCTTCGCTGGGAC
  CACGTTTGAATACCAGCGCTCTTTCAACCGCCCGGAACGTCTGTACGCGCCAGGGCCCACAAATGAGACG
  CTGGTCTTTGAAATTCTGATGCAAGGCAAAAATCCAGGGATAGCTTGGAAGTATGCACTTCCCAAGGTCA
  TGAATGGAACTCCACCAGCCACAAAAAGACCTGCCTATACCTGGAGTATCGTGCAGTCAGAGTGCTCCGT
  CTCCTGTGGTGGAGGTTACATAAATGTAAAGGCCATTTGCTTGCGAGATCAAAATACTCAAGTCAATTCC
  TCATTCTGCAGTGCAAAAACCAAGCCAGTAACTGAGCCCAAAATCTGCAACGCTTTCTCCTGCCCGGCTT
  ACTGGATGCCAGGTGAATGGAGTACATGCAGCAAGGCCTGTGCTGGAGGCCAGCAGAGCCGAAAGATCCA
  GTGTGTGCAAAAGAAGCCCTTCCAAAAGGAGGAAGCAGTGTTGCATTCTCTCTGTCCAGTGAGCACACCC
  ACTCAGGTCCAAGCCTGCAACAGCCATGCCTGCCCTCCACAATGGAGCCTTGGACCCTGGTCTCAGTGTT
  CCAAGACCTGTGGACGAGGGGTGAGGAAGCGTGAACTCCTCTGCAAGGGCTCTGCCGCAGAAACCCTCCC
  CGAGAGCCAGTGTACCAGTCTCCCCAGACCTGAGCTGCAGGAGGGCTGTGTGCTTGGACGATGCCCCAAG
  AACAGCCGGCTACAGTGGGTCGCTTCTTCGTGGAGCGAGTGTTCTGCAACCTGTGGTTTGGGTGTGAGGA
  AGAGGGAGATGAAGTGCAGCGAGAAGGGCTTCCAGGGAAAGCTGATAACTTTCCCAGAGCGAAGATGCCG
  TAATATTAAGAAACCAAATCTGGACTTGGAAGAGACCTGCAACCGACGGGCTTGCCCAGCCCATCCAGTG
  TACAACATGGTAGCTGGATGGTATTCATTGCCGTGGCAGCAGTGCACAGTCACCTGTGGGGGAGGGGTCC
  AGACCCGGTCAGTCCACTGTGTTCAGCAAGGCCGGCCTTCCTCAAGTTGTCTGCTCCATCAGAAACCTCC
  GGTGCTACGAGCCTGTAATACAAACTTCTGTCCAGCTCCTGAAAAGAGAGAGGATCCATCCTGCGTAGAT
  TTCTTCAACTGGTGTCACCTAGTTCCTCAGCATGGTGTCTGCAACCACAAGTTTTACGGAAAACAATGCT
  GCAAGTCATGCACAAGGAAGATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209192 representing NM_199355
  Red=Cloning site Green=Tags(s)
  
  MECALLLACAFPAAGSGPPRGLAGLGRVAKALQLCCLCCASVAAALASDSSSGASGLNDDYVFVTPVEVD
  SAGSYISHDILHNGRKKRSAQNARSSLHYRFSAFGQELHLELKPSAILSSHFIVQVLGKDGASETQKPEV
  QQCFYQGFIRNDSSSSVAVSTCAGLSGLIRTRKNEFLISPLPQLLAQEHNHSSPAGHHPHVLYKRTAEEK
  IQRYRGYPGSGRNYPGYSPSHIPHASQSRETEYHHRRLQKQHFCGRRKKYAPKPPTEDTYLRFDEYGSSG
  RPRRSAGKSQKGLNVETLVVADKKMVEKHGKGNVTTYILTVMNMVSGLFKDGTIGSDINVVVVSLILLEQ
  EPGGLLINHHADQSLNSFCQWQSALIGKNGKRHDHAILLTGFDICSWKNEPCDTLGFAPISGMCSKYRSC
  TINEDTGLGLAFTIAHESGHNFGMIHDGEGNPCRKAEGNIMSPTLTGNNGVFSWSSCSRQYLKKFLSTPQ
  AGCLVDEPKQAGQYKYPDKLPGQIYDADTQCKWQFGAKAKLCSLGFVKDICKSLWCHRVGHRCETKFMPA
  AEGTVCGLSMWCRQGQCVKFGELGPRPIHGQWSAWSKWSECSRTCGGGVKFQERHCNNPKPQYGGIFCPG
  SSRIYQLCNINPCNENSLDFRAQQCAEYNSKPFRGWFYQWKPYTKVEEEDRCKLYCKAENFEFFFAMSGK
  VKDGTPCSPNKNDVCIDGVCELVGCDHELGSKAVSDACGVCKGDNSTCKFYKGLYLNQHKANEYYPVVII
  PAGARSIEIQELQVSSSYLAVRSLSQKYYLTGGWSIDWPGEFPFAGTTFEYQRSFNRPERLYAPGPTNET
  LVFEILMQGKNPGIAWKYALPKVMNGTPPATKRPAYTWSIVQSECSVSCGGGYINVKAICLRDQNTQVNS
  SFCSAKTKPVTEPKICNAFSCPAYWMPGEWSTCSKACAGGQQSRKIQCVQKKPFQKEEAVLHSLCPVSTP
  TQVQACNSHACPPQWSLGPWSQCSKTCGRGVRKRELLCKGSAAETLPESQCTSLPRPELQEGCVLGRCPK
  NSRLQWVASSWSECSATCGLGVRKREMKCSEKGFQGKLITFPERRCRNIKKPNLDLEETCNRRACPAHPV
  YNMVAGWYSLPWQQCTVTCGGGVQTRSVHCVQQGRPSSSCLLHQKPPVLRACNTNFCPAPEKREDPSCVD
  FFNWCHLVPQHGVCNHKFYGKQCCKSCTRKI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_199355
• ORF Size: 3663 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_199355.4
• RefSeq Size: 5510 bp
• RefSeq ORF: 3666 bp
• Locus ID: 170692
• UniProt ID: Q8TE60
• Cytogenetics: 16q23.1
• Protein Families: Protease, Secreted Protein
• MW: 135 kDa
• Gene Summary: This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. ADAMTS family members share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The encoded preproprotein is proteolytically processed to generate the mature protein, which may regulate hemostatic balance and function as a tumor suppressor. Mutations in this gene may be associated with microcornea, myopic chorioretinal atrophy, and telecanthus (MMCAT) and cone-rod dystrophy in human patients. [provided by RefSeq, May 2016]