FOXL2 (NM_023067) Human Tagged ORF Clone

CAT#: RC209127

FOXL2 (Myc-DDK-tagged)-Human forkhead box L2 (FOXL2)

Product Images

Circular map for RC209127

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  Western blot validation of overexpression lysate (Cat# LY411500) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC209127 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified FOXL2 protein (Cat# TP309127). The protein was produced from HEK293T cells transfected with FOXL2 cDNA clone (Cat# RC209127) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FOXL2
• Synonyms: BPES; BPES1; PFRK; PINTO; POF3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209127 representing NM_023067
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGGCCAGCTACCCCGAGCCCGAGGACGCGGCGGGGGCCCTGCTGGCCCCAGAGACCGGTCGCACAG
  TCAAGGAGCCAGAAGGGCCGCCGCCGAGCCCAGGCAAGGGCGGTGGGGGTGGCGGCGGGACAGCCCCGGA
  GAAGCCGGACCCGGCGCAGAAGCCCCCGTACTCGTACGTGGCGCTCATCGCCATGGCGATCCGCGAGAGC
  GCGGAGAAGAGGCTCACGCTGTCCGGCATCTACCAGTACATCATCGCGAAGTTCCCGTTCTACGAGAAGA
  ATAAGAAGGGCTGGCAAAATAGCATCCGCCACAACCTCAGCCTCAACGAGTGCTTCATCAAGGTGCCGCG
  CGAGGGCGGCGGCGAGCGCAAGGGCAACTACTGGACGCTGGACCCGGCCTGCGAAGACATGTTCGAGAAG
  GGCAACTACCGGCGCCGCCGCCGCATGAAGAGGCCCTTCCGGCCGCCGCCCGCGCACTTCCAGCCCGGCA
  AGGGGCTCTTCGGGGCCGGAGGCGCCGCAGGCGGGTGCGGCGTGGCGGGCGCCGGGGCCGACGGCTACGG
  CTACCTGGCGCCCCCCAAGTACCTGCAGTCTGGCTTCCTCAACAACTCGTGGCCGCTACCGCAGCCTCCC
  TCACCCATGCCCTATGCCTCCTGCCAGATGGCGGCAGCCGCAGCGGCTGCAGCAGCTGCGGCTGCAGCCG
  CGGGCCCCGGTAGCCCTGGCGCGGCCGCTGTGGTCAAGGGGCTGGCGGGCCCGGCCGCCTCGTACGGGCC
  GTACACACGCGTGCAGAGCATGGCGCTGCCCCCCGGCGTAGTGAACTCGTACAATGGCCTGGGAGGCCCG
  CCGGCCGCACCCCCGCCTCCGCCGCACCCCCACCCGCATCCGCACGCACACCATCTGCACGCGGCCGCCG
  CACCGCCGCCTGCCCCACCGCACCACGGGGCCGCCGCGCCGCCGCCGGGCCAGCTCAGCCCTGCCAGCCC
  AGCCACCGCCGCGCCCCCGGCGCCCGCGCCCACCAGTGCGCCGGGCCTGCAGTTCGCTTGTGCCCGGCAG
  CCCGAGCTCGCCATGATGCATTGCTCTTACTGGGACCACGACAGCAAGACCGGCGCGCTGCATTCGCGCC
  TCGATCTC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209127 representing NM_023067
  Red=Cloning site Green=Tags(s)
  
  MMASYPEPEDAAGALLAPETGRTVKEPEGPPPSPGKGGGGGGGTAPEKPDPAQKPPYSYVALIAMAIRES
  AEKRLTLSGIYQYIIAKFPFYEKNKKGWQNSIRHNLSLNECFIKVPREGGGERKGNYWTLDPACEDMFEK
  GNYRRRRRMKRPFRPPPAHFQPGKGLFGAGGAAGGCGVAGAGADGYGYLAPPKYLQSGFLNNSWPLPQPP
  SPMPYASCQMAAAAAAAAAAAAAAGPGSPGAAAVVKGLAGPAASYGPYTRVQSMALPPGVVNSYNGLGGP
  PAAPPPPPHPHPHPHAHHLHAAAAPPPAPPHHGAAAPPPGQLSPASPATAAPPAPAPTSAPGLQFACARQ
  PELAMMHCSYWDHDSKTGALHSRLDL
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_023067
• ORF Size: 1128 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_023067.4
• RefSeq Size: 2744 bp
• RefSeq ORF: 1131 bp
• Locus ID: 668
• UniProt ID: P58012
• Cytogenetics: 3q22.3
• Protein Families: Druggable Genome, Transcription Factors
• MW: 38.6 kDa
• Gene Summary: This gene encodes a forkhead transcription factor. The protein contains a fork-head DNA-binding domain and may play a role in ovarian development and function. Expansion of a polyalanine repeat region and other mutations in this gene are a cause of blepharophimosis syndrome and premature ovarian failure 3. [provided by RefSeq, Jul 2016]