Chymotrypsin like protease (CTRL) (NM_001907) Human Tagged ORF Clone

CAT#: RC209072

CTRL (Myc-DDK-tagged)-Human chymotrypsin-like (CTRL)

Product Images

Circular map for RC209072

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY CTRL (Cat# RC209072, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CTRL antibody (Cat# TA890107). Positive lysates LY419665 (100ug) and LC419665 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY419665) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC209072 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified CTRL protein (Cat# TP309072). The protein was produced from HEK293T cells transfected with CTRL cDNA clone (Cat# RC209072) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Chymotrypsin like protease
• Synonyms: CTRL1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209072 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTGCTGCTCAGCCTGACCCTAAGCCTGGTTCTCCTCGGCTCCTCCTGGGGCTGCGGCATTCCTGCCA
  TCAAACCGGCACTGAGCTTCAGCCAGAGGATTGTCAACGGGGAGAATGCAGTGTTGGGCTCCTGGCCCTG
  GCAGGTGTCCCTGCAGGACAGCAGCGGCTTCCACTTCTGCGGTGGTTCTCTCATCAGCCAGTCCTGGGTG
  GTCACTGCTGCCCACTGCAATGTCAGCCCTGGCCGCCATTTTGTTGTCCTGGGCGAGTATGACCGATCAT
  CAAACGCAGAGCCCTTGCAGGTTCTGTCCGTCTCTCGGGCCATTACACACCCTAGCTGGAACTCTACCAC
  CATGAACAATGACGTGACGCTGCTGAAGCTCGCCTCGCCAGCCCAGTACACAACACGCATCTCGCCAGTT
  TGCCTGGCATCCTCAAACGAGGCTCTGACTGAAGGCCTCACGTGTGTCACCACCGGCTGGGGTCGCCTCA
  GTGGCGTGGGCAATGTGACACCAGCACATCTGCAGCAGGTGGCTTTGCCCCTGGTCACTGTGAATCAGTG
  CCGGCAGTACTGGGGCTCAAGTATCACTGACTCCATGATCTGTGCAGGTGGCGCAGGTGCCTCCTCGTGC
  CAGGGTGACTCCGGAGGCCCTCTTGTCTGCCAGAAGGGAAACACATGGGTGCTTATTGGTATTGTCTCCT
  GGGGCACCAAAAACTGCAATGTGCGCGCACCTGCTGTGTATACTCGAGTTAGCAAGTTCAGCACCTGGAT
  CAACCAGGTCATAGCCTACAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209072 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MLLLSLTLSLVLLGSSWGCGIPAIKPALSFSQRIVNGENAVLGSWPWQVSLQDSSGFHFCGGSLISQSWV
  VTAAHCNVSPGRHFVVLGEYDRSSNAEPLQVLSVSRAITHPSWNSTTMNNDVTLLKLASPAQYTTRISPV
  CLASSNEALTEGLTCVTTGWGRLSGVGNVTPAHLQQVALPLVTVNQCRQYWGSSITDSMICAGGAGASSC
  QGDSGGPLVCQKGNTWVLIGIVSWGTKNCNVRAPAVYTRVSKFSTWINQVIAYN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001907
• ORF Size: 792 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001907.3
• RefSeq Size: 1197 bp
• RefSeq ORF: 795 bp
• Locus ID: 1506
• UniProt ID: P40313
• Cytogenetics: 16q22.1
• Protein Families: Druggable Genome, Protease
• MW: 28 kDa
• Gene Summary: This gene encodes a serine-type endopeptidase with chymotrypsin- and elastase-2-like activities. The gene encoding this zymogen is expressed specifically in the pancreas and likely functions as a digestive enzyme. [provided by RefSeq, Sep 2016]