AFG3L2 (NM_006796) Human Tagged ORF Clone

CAT#: RC209058

AFG3L2 (Myc-DDK-tagged)-Human AFG3 ATPase family gene 3-like 2 (S. cerevisiae) (AFG3L2), nuclear gene encoding mitochondrial protein

Product Images

Circular map for RC209058

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY AFG3L2 (Cat# RC209058, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AFG3L2 (Cat# TA504858). Positive lysates LY416420 (100ug) and LC416420 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY416420) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC209058 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: AFG3L2
• Synonyms: OPA12; SCA28; SPAX5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209058 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCACCGCTGTTTGCGGCTGTGGGGCCGGGGCGGCTGCTGGCCCCGCGGCCTACAGCAGCTCCTCG
  TGCCTGGCGGCGTGGGCCCGGGCGAGCAGCCCTGCCTCCGGACGCTTTACCGATTTGTTACAACTCAAGC
  AAGGGCCAGCAGAAATTCTCTTTTGACAGATATAATTGCTGCTTATCAAAGATTCTGTTCTCGACCCCCA
  AAAGGATTTGAAAAATACTTTCCTAATGGAAAAAATGGAAAAAAAGCTAGTGAACCTAAAGAAGTTATGG
  GAGAGAAAAAAGAATCAAAGCCAGCTGCTACCACACGCTCTTCTGGAGGAGGAGGTGGTGGCGGTGGAAA
  ACGAGGTGGCAAGAAAGATGATTCTCACTGGTGGTCCAGGTTTCAGAAGGGTGACATTCCATGGGACGAC
  AAGGATTTCAGGATGTTCTTCCTCTGGACTGCTCTGTTCTGGGGTGGAGTCATGTTTTACTTGCTGCTCA
  AGAGATCCGGGAGAGAAATCACTTGGAAGGACTTTGTCAATAACTATCTTTCAAAAGGAGTAGTAGACAG
  ATTGGAAGTCGTCAACAAGCGTTTTGTTCGAGTGACCTTTACACCAGGAAAAACTCCTGTTGATGGGCAA
  TACGTTTGGTTTAATATTGGCAGTGTGGACACCTTTGAACGGAATCTGGAAACTTTACAGCAGGAATTGG
  GCATAGAAGGAGAAAATCGGGTGCCTGTTGTCTACATTGCTGAAAGTGATGGCTCTTTTCTGCTGAGCAT
  GCTGCCTACGGTGCTCATCATCGCCTTCTTGCTCTACACCATCAGAAGAGGGCCTGCTGGCATTGGCCGG
  ACAGGCCGAGGGATGGGCGGACTCTTCAGTGTCGGAGAAACCACTGCCAAGGTCTTAAAGGATGAAATTG
  ATGTGAAGTTCAAAGATGTGGCTGGCTGTGAGGAGGCCAAGCTAGAGATCATGGAATTTGTGAATTTCTT
  GAAAAACCCAAAGCAGTATCAAGACCTAGGAGCAAAAATCCCAAAGGGTGCCATTCTCACTGGTCCTCCA
  GGCACTGGGAAGACGCTGCTAGCTAAGGCCACAGCCGGAGAAGCCAATGTCCCCTTCATCACCGTTAGTG
  GATCTGAGTTTTTGGAGATGTTCGTTGGTGTGGGCCCTGCTAGAGTCCGAGACTTATTTGCCCTTGCTCG
  GAAGAATGCCCCTTGCATCCTCTTCATCGATGAAATCGATGCGGTGGGAAGGAAGAGAGGAAGAGGCAAC
  TTTGGAGGGCAGAGTGAGCAGGAGAACACACTCAACCAGCTGCTGGTGGAGATGGATGGTTTTAATACAA
  CAACAAATGTCGTCATTTTGGCCGGCACCAATCGACCAGATATCCTGGACCCCGCGCTACTTAGGCCGGG
  GCGTTTCGACAGGCAGATCTTTATTGGACCACCAGACATAAAAGGAAGAGCTTCTATTTTCAAAGTTCAT
  CTCCGACCGCTAAAACTGGACAGTACCCTGGAGAAGGATAAATTGGCAAGAAAACTGGCATCTTTAACTC
  CAGGGTTTTCAGGTGCTGATGTTGCTAATGTCTGTAATGAAGCTGCGTTGATTGCTGCAAGGCATCTGTC
  AGATTCCATAAATCAGAAACACTTTGAACAGGCAATTGAGCGAGTGATTGGTGGCTTAGAGAAGAAAACG
  CAGGTTCTGCAGCCTGAGGAGAAGAAGACTGTGGCATACCACGAAGCAGGCCATGCGGTTGCCGGCTGGT
  ATCTGGAGCACGCAGACCCGCTTTTAAAGGTATCCATCATCCCACGTGGCAAAGGACTAGGTTATGCTCA
  GTATTTACCAAAAGAACAATACCTCTATACCAAAGAGCAGCTCTTGGATAGGATGTGTATGACTTTAGGT
  GGTCGAGTCTCTGAAGAAATCTTCTTTGGAAGAATTACAACTGGTGCTCAAGATGACTTGAGAAAAGTAA
  CTCAGAGTGCATATGCCCAAATTGTTCAGTTTGGCATGAATGAAAAGGTTGGGCAAATCTCCTTTGACCT
  CCCACGTCAGGGGGACATGGTATTGGAGAAACCTTACAGTGAAGCCACTGCAAGATTGATAGATGATGAA
  GTACGAATACTTATTAATGATGCTTATAAAAGAACAGTAGCTCTTCTCACAGAAAAGAAAGCTGACGTGG
  AGAAGGTTGCTCTTCTGTTGTTAGAAAAAGAAGTATTAGATAAGAATGATATGGTTGAACTTTTGGGCCC
  CAGACCATTTGCGGAAAAATCTACCTATGAAGAATTTGTGGAAGGCACTGGCAGCTTGGATGAGGACACC
  TCACTTCCAGAAGGCCTTAAGGACTGGAACAAGGAGCGGGAAAAGGAGAAAGAGGAGCCCCCGGGTGAGA
  AAGTTGCCAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209058 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAHRCLRLWGRGGCWPRGLQQLLVPGGVGPGEQPCLRTLYRFVTTQARASRNSLLTDIIAAYQRFCSRPP
  KGFEKYFPNGKNGKKASEPKEVMGEKKESKPAATTRSSGGGGGGGGKRGGKKDDSHWWSRFQKGDIPWDD
  KDFRMFFLWTALFWGGVMFYLLLKRSGREITWKDFVNNYLSKGVVDRLEVVNKRFVRVTFTPGKTPVDGQ
  YVWFNIGSVDTFERNLETLQQELGIEGENRVPVVYIAESDGSFLLSMLPTVLIIAFLLYTIRRGPAGIGR
  TGRGMGGLFSVGETTAKVLKDEIDVKFKDVAGCEEAKLEIMEFVNFLKNPKQYQDLGAKIPKGAILTGPP
  GTGKTLLAKATAGEANVPFITVSGSEFLEMFVGVGPARVRDLFALARKNAPCILFIDEIDAVGRKRGRGN
  FGGQSEQENTLNQLLVEMDGFNTTTNVVILAGTNRPDILDPALLRPGRFDRQIFIGPPDIKGRASIFKVH
  LRPLKLDSTLEKDKLARKLASLTPGFSGADVANVCNEAALIAARHLSDSINQKHFEQAIERVIGGLEKKT
  QVLQPEEKKTVAYHEAGHAVAGWYLEHADPLLKVSIIPRGKGLGYAQYLPKEQYLYTKEQLLDRMCMTLG
  GRVSEEIFFGRITTGAQDDLRKVTQSAYAQIVQFGMNEKVGQISFDLPRQGDMVLEKPYSEATARLIDDE
  VRILINDAYKRTVALLTEKKADVEKVALLLLEKEVLDKNDMVELLGPRPFAEKSTYEEFVEGTGSLDEDT
  SLPEGLKDWNKEREKEKEEPPGEKVAN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006796
• ORF Size: 2391 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006796.3
• RefSeq Size: 3223 bp
• RefSeq ORF: 2394 bp
• Locus ID: 10939
• UniProt ID: Q9Y4W6
• Cytogenetics: 18p11.21
• Domains: Peptidase_M41, AAA, AAA
• Protein Families: Druggable Genome, Protease, Transmembrane
• MW: 88.6 kDa
• Gene Summary: This gene encodes a protein localized in mitochondria and closely related to paraplegin. The paraplegin gene is responsible for an autosomal recessive form of hereditary spastic paraplegia. This gene is a candidate gene for other hereditary spastic paraplegias or neurodegenerative disorders. [provided by RefSeq, Jul 2008]