SCYL2 (NM_017988) Human Tagged ORF Clone

CAT#: RC209051

SCYL2 (Myc-DDK-tagged)-Human SCY1-like 2 (S. cerevisiae) (SCYL2)

Product Images

Circular map for RC209051

• 

  Western blot validation of overexpression lysate (Cat# LY413396) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC209051 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SCYL2
• Synonyms: AMC4; AMCNACC; CVAK104
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209051 representing NM_017988
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGTCCATGCTTAATAAATTGAAGAGTACTGTTACAAAAGTAACAGCTGATGTCACTAGTGCTGTAA
  TGGGAAATCCTGTCACTAGAGAATTTGATGTTGGTCGACACATTGCCAGTGGTGGCAATGGGCTAGCTTG
  GAAGATTTTTAATGGCACAAAAAAGTCAACAAAGCAGGAAGTGGCAGTTTTTGTCTTTGATAAAAAACTG
  ATTGACAAGTATCAAAAATTTGAAAAGGATCAAATCATTGATTCTCTAAAACGAGGAGTCCAACAGTTAA
  CTCGGCTTCGACACCCTCGACTTCTTACTGTCCAGCATCCTTTAGAAGAATCCAGGGATTGCTTGGCATT
  TTGTACAGAACCAGTTTTTGCCAGTTTAGCCAATGTTCTTGGTAACTGGGAAAATCTACCTTCCCCTATA
  TCTCCAGACATTAAGGATTATAAACTTTATGATGTAGAAACCAAATATGGTTTGCTTCAGGTTTCTGAAG
  GATTGTCATTCTTGCATAGCAGTGTGAAAATGGTGCATGGAAATATCACTCCTGAAAATATAATTTTGAA
  TAAAAGTGGAGCCTGGAAAATAATGGGTTTTGATTTTTGTGTATCATCAACCAATCCTTCTGAACAAGAG
  CCTAAATTTCCTTGTAAAGAATGGGACCCAAATTTACCTTCATTGTGTCTTCCAAATCCTGAATATTTGG
  CTCCTGAATACATACTTTCTGTGAGCTGTGAAACAGCCAGTGATATGTATTCTTTAGGAACTGTTATGTA
  TGCTGTATTTAATAAAGGGAAACCTATATTTGAAGTCAACAAGCAAGATATTTACAAGAGTTTCAGTAGG
  CAGTTGGATCAGTTGAGTCGTTTAGGATCTAGTTCACTTACAAATATACCTGAGGAAGTTCGTGAACATG
  TAAAGCTACTGTTAAATGTAACTCCGACTGTAAGACCAGATGCAGATCAAATGACAAAGATTCCCTTCTT
  TGATGATGTTGGTGCAGTAACACTGCAATATTTTGATACCTTATTCCAAAGAGATAATCTTCAGAAATCA
  CAGTTTTTCAAAGGACTGCCAAAGGTTCTACCAAAACTGCCCAAGCGTGTCATTGTGCAGAGAATTTTGC
  CTTGTTTGACTTCAGAATTTGTAAACCCTGACATGGTACCTTTTGTTTTGCCCAATGTTCTACTTATTGC
  TGAGGAATGCACCAAAGAAGAATATGTCAAATTAATTCTTCCTGAACTTGGCCCTGTGTTTAAGCAGCAG
  GAGCCAATCCAGATTTTGTTAATTTTCCTACAAAAAATGGATTTGCTACTAACCAAAACCCCTCCTGATG
  AGATAAAGAACAGTGTTCTACCCATGGTTTACAGAGCACTAGAAGCTCCTTCCATTCAGATCCAGGAGCT
  CTGTCTAAACATCATTCCAACCTTTGCAAATCTTATAGACTACCCATCCATGAAAAACGCTTTGATACCA
  AGAATTAAAAATGCTTGTCTACAAACATCTTCCCTTGCGGTTCGTGTAAATTCATTAGTGTGCTTAGGAA
  AGATTTTGGAATACTTGGATAAGTGGTTTGTACTTGATGATATCCTACCCTTCTTACAACAAATTCCATC
  CAAGGAACCTGCGGTCCTCATGGGAATTTTAGGTATTTACAAATGTACTTTTACTCATAAGAAGTTGGGA
  ATCACCAAAGAGCAGCTGGCCGGAAAAGTGTTGCCTCATCTTATTCCCCTGAGTATTGAAAACAATCTTA
  ATCTTAATCAGTTCAATTCTTTCATTTCCGTCATAAAAGAAATGCTTAATAGATTGGAGTCTGAACATAA
  GACTAAACTGGAGCAACTTCATATAATGCAAGAACAGCAGAAATCTTTGGATATAGGAAATCAAATGAAT
  GTTTCTGAGGAGATGAAAGTTACAAATATTGGGAATCAGCAAATTGACAAAGTTTTTAACAACATTGGAG
  CAGACCTTCTGACTGGCAGTGAGTCCGAAAATAAAGAGGACGGGTTACAGAATAAACATAAAAGAGCATC
  ACTTACACTTGAAGAAAAACAAAAATTAGCAAAAGAACAAGAGCAGGCACAGAAGCTGAAAAGCCAGCAG
  CCTCTTAAACCCCAAGTGCACACACCTGTTGCTACTGTTAAACAGACTAAGGACTTGACAGACACACTGA
  TGGATAATATGTCATCCTTGACCAGCCTTTCTGTTAGTACCCCTAAATCTTCTGCTTCAAGTACTTTCAC
  TTCTGTTCCTTCCATGGGCATTGGTATGATGTTTTCTACACCAACTGATAATACAAAGAGAAATTTGACA
  AATGGCCTAAATGCCAATATGGGCTTTCAGACTTCAGGATTCAACATGCCCGTTAATACAAACCAGAACT
  TCTACAGTAGTCCAAGCACAGTTGGAGTGACCAAGATGACTCTGGGAACACCTCCCACTTTGCCAAACTT
  CAATGCTTTGAGTGTTCCTCCTGCTGGTGCAAAGCAGACCCAACAAAGACCCACAGATATGTCTGCCCTT
  AATAATCTCTTTGGCCCTCAGAAACCCAAAGTTAGCATGAACCAGTTATCACAACAGAAACCAAATCAGT
  GGCTTAATCAGTTTGTACCTCCTCAAGGTTCTCCAACTATGGGCAGTTCAGTAATGGGGACACAGATGAA
  CGTGATAGGACAATCTGCTTTTGGTATGCAGGGTAATCCTTTCTTTAACCCACAGAACTTTGCACAGCCA
  CCAACTACTATGACCAATAGCAGTTCAGCTAGCAATGATTTAAAAGATCTTTTTGGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209051 representing NM_017988
  Red=Cloning site Green=Tags(s)
  
  MESMLNKLKSTVTKVTADVTSAVMGNPVTREFDVGRHIASGGNGLAWKIFNGTKKSTKQEVAVFVFDKKL
  IDKYQKFEKDQIIDSLKRGVQQLTRLRHPRLLTVQHPLEESRDCLAFCTEPVFASLANVLGNWENLPSPI
  SPDIKDYKLYDVETKYGLLQVSEGLSFLHSSVKMVHGNITPENIILNKSGAWKIMGFDFCVSSTNPSEQE
  PKFPCKEWDPNLPSLCLPNPEYLAPEYILSVSCETASDMYSLGTVMYAVFNKGKPIFEVNKQDIYKSFSR
  QLDQLSRLGSSSLTNIPEEVREHVKLLLNVTPTVRPDADQMTKIPFFDDVGAVTLQYFDTLFQRDNLQKS
  QFFKGLPKVLPKLPKRVIVQRILPCLTSEFVNPDMVPFVLPNVLLIAEECTKEEYVKLILPELGPVFKQQ
  EPIQILLIFLQKMDLLLTKTPPDEIKNSVLPMVYRALEAPSIQIQELCLNIIPTFANLIDYPSMKNALIP
  RIKNACLQTSSLAVRVNSLVCLGKILEYLDKWFVLDDILPFLQQIPSKEPAVLMGILGIYKCTFTHKKLG
  ITKEQLAGKVLPHLIPLSIENNLNLNQFNSFISVIKEMLNRLESEHKTKLEQLHIMQEQQKSLDIGNQMN
  VSEEMKVTNIGNQQIDKVFNNIGADLLTGSESENKEDGLQNKHKRASLTLEEKQKLAKEQEQAQKLKSQQ
  PLKPQVHTPVATVKQTKDLTDTLMDNMSSLTSLSVSTPKSSASSTFTSVPSMGIGMMFSTPTDNTKRNLT
  NGLNANMGFQTSGFNMPVNTNQNFYSSPSTVGVTKMTLGTPPTLPNFNALSVPPAGAKQTQQRPTDMSAL
  NNLFGPQKPKVSMNQLSQQKPNQWLNQFVPPQGSPTMGSSVMGTQMNVIGQSAFGMQGNPFFNPQNFAQP
  PTTMTNSSSASNDLKDLFG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017988
• ORF Size: 2787 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017988.6
• RefSeq Size: 3818 bp
• RefSeq ORF: 2790 bp
• Locus ID: 55681
• UniProt ID: Q6P3W7
• Cytogenetics: 12q23.1
• Protein Families: Druggable Genome, Protein Kinase
• MW: 103.5 kDa
• Gene Summary: The protein encoded by this gene associates with clathrin-coated complexes at the plasma membrane and with endocytic coated vesicles. The encoded protein phosphorylates the beta2 subunit of the plasma membrane adapter complex AP2 and interacts with clathrin, showing involvement in clathrin-dependent pathways between the trans-Golgi network and the endosomal system. In addition, this protein has a role in the Wnt signaling pathway by targeting frizzled 5 (Fzd5) for lysosomal degradation. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Dec 2015]