VANGL1 (NM_138959) Human Tagged ORF Clone

CAT#: RC209024

VANGL1 (Myc-DDK-tagged)-Human vang-like 1 (van gogh, Drosophila) (VANGL1), transcript variant 1

Product Images

Circular map for RC209024

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  Western blot validation of overexpression lysate (Cat# LY403377) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC209024 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified VANGL1 protein (Cat# TP309024). The protein was produced from HEK293T cells transfected with VANGL1 cDNA clone (Cat# RC209024) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: VANGL1
• Synonyms: KITENIN; LPP2; STB2; STBM2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209024 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATACCGAATCCACTTATTCTGGATATTCTTACTATTCAAGTCATTCGAAAAAATCTCACAGACAAG
  GGGAAAGAACTAGAGAGAGACACAAGTCACCCCGGAATAAAGACGGCAGAGGGTCAGAAAAGTCTGTCAC
  CATTCAACCTCCCACTGGAGAGCCCCTGTTGGGAAATGATTCTACTCGGACAGAGGAAGTTCAGGATGAC
  AACTGGGGAGAGACCACCACGGCCATCACAGGCACCTCGGAGCACAGCATATCCCAAGAGGACATTGCCA
  GGATCAGCAAGGACATGGAGGACAGCGTGGGGCTGGATTGCAAACGCTACCTGGGCCTCACCGTCGCCTC
  TTTTCTTGGACTTCTAGTTTTCCTCACCCCTATTGCCTTCATCCTTTTACCTCCGATCCTGTGGAGGGAT
  GAGCTGGAGCCTTGTGGCACAATTTGTGAGGGGCTCTTTATCTCCATGGCATTCAAACTCCTCATTCTGC
  TCATAGGGACCTGGGCACTTTTTTTCCGCAAGCGGAGAGCTGACATGCCACGGGTGTTTGTGTTTCGTGC
  CCTTTTGTTGGTCCTCATCTTTCTCTTTGTGGTTTCCTATTGGCTTTTTTACGGGGTCCGCATTTTGGAC
  TCTCGGGACCGGAATTACCAGGGCATTGTGCAATATGCAGTCTCCCTTGTGGATGCCCTCCTCTTCATCC
  ATTACCTGGCCATCGTCCTGCTGGAGCTCAGGCAGCTGCAGCCCATGTTCACGCTGCAGGTGGTCCGCTC
  CACCGATGGCGAGTCCCGCTTCTACAGCCTGGGACACCTGAGTATCCAGCGAGCAGCATTGGTGGTCCTA
  GAAAATTACTACAAAGATTTCACCATCTATAACCCAAACCTCCTAACAGCCTCCAAATTCCGAGCAGCCA
  AGCATATGGCCGGGCTGAAAGTCTACAATGTAGATGGCCCCAGTAACAATGCCACTGGCCAGTCCCGGGC
  CATGATTGCTGCAGCTGCTCGGCGCAGGGACTCAAGCCACAACGAGTTGTATTATGAAGAGGCCGAACAT
  GAACGGCGAGTAAAGAAGCGGAAAGCAAGGCTGGTGGTTGCAGTGGAAGAGGCCTTCATCCACATTCAGC
  GTCTCCAGGCTGAGGAGCAGCAGAAAGCCCCAGGGGAGGTGATGGACCCTAGGGAGGCCGCCCAGGCCAT
  TTTCCCCTCCATGGCCAGGGCTCTCCAGAAGTACCTGCGCATCACCCGGCAGCAGAACTACCACAGCATG
  GAGAGCATCCTGCAGCACCTGGCCTTCTGCATCACCAACGGCATGACCCCCAAGGCCTTCCTAGAACGGT
  ACCTCAGTGCGGGCCCCACCCTGCAATATGACAAGGACCGCTGGCTCTCTACACAGTGGAGGCTTGTCAG
  TGATGAGGCTGTGACTAATGGATTACGGGATGGAATTGTGTTCGTCCTTAAGTGCTTGGACTTCAGCCTC
  GTAGTCAATGTGAAGAAAATTCCATTCATCATACTCTCTGAAGAGTTCATAGACCCCAAATCTCACAAAT
  TTGTCCTTCGCTTACAGTCTGAGACATCCGTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209024 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDTESTYSGYSYYSSHSKKSHRQGERTRERHKSPRNKDGRGSEKSVTIQPPTGEPLLGNDSTRTEEVQDD
  NWGETTTAITGTSEHSISQEDIARISKDMEDSVGLDCKRYLGLTVASFLGLLVFLTPIAFILLPPILWRD
  ELEPCGTICEGLFISMAFKLLILLIGTWALFFRKRRADMPRVFVFRALLLVLIFLFVVSYWLFYGVRILD
  SRDRNYQGIVQYAVSLVDALLFIHYLAIVLLELRQLQPMFTLQVVRSTDGESRFYSLGHLSIQRAALVVL
  ENYYKDFTIYNPNLLTASKFRAAKHMAGLKVYNVDGPSNNATGQSRAMIAAAARRRDSSHNELYYEEAEH
  ERRVKKRKARLVVAVEEAFIHIQRLQAEEQQKAPGEVMDPREAAQAIFPSMARALQKYLRITRQQNYHSM
  ESILQHLAFCITNGMTPKAFLERYLSAGPTLQYDKDRWLSTQWRLVSDEAVTNGLRDGIVFVLKCLDFSL
  VVNVKKIPFIILSEEFIDPKSHKFVLRLQSETSV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_138959
• ORF Size: 1572 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_138959.3
• RefSeq Size: 8691 bp
• RefSeq ORF: 1575 bp
• Locus ID: 81839
• UniProt ID: Q8TAA9
• Cytogenetics: 1p13.1
• Protein Families: Transmembrane
• Protein Pathways: Wnt signaling pathway
• MW: 60 kDa
• Gene Summary: This gene encodes a member of the tretraspanin family. The encoded protein may be involved in mediating intestinal trefoil factor induced wound healing in the intestinal mucosa. Mutations in this gene are associated with neural tube defects. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Feb 2010]