SUHW1 (ZNF280A) (NM_080740) Human Tagged ORF Clone

CAT#: RC208802

ZNF280A (Myc-DDK-tagged)-Human zinc finger protein 280A (ZNF280A)

Product Images

Circular map for RC208802

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY ZNF280A (Cat# RC208802, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ZNF280A(Cat# TA800173). Positive lysates LY409057 (100ug) and LC409057 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY409057) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC208802 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SUHW1
• Synonyms: 3'OY11.1; SUHW1; ZNF280; ZNF636
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208802 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAGATATCTTTTTGTGTAAGAAAGTGGAATCACCAAAGAAGAATTTGAGAGAATCCAAACAAAGGG
  AGGAGGATGATGAAGATCCAGATCTGATCTATGTTGGGGTGGAGCATGTACATAGAGATGCTGAAGTTCT
  CTTTGTCGGGATGATTTCAAATTCAAAACCAGTCGTTTCAAACATTTTGAACAGAGTCACCCCAGGCTCA
  AATTCAAGAAGAAAGAAAGGCCACTTCCGTCAATATCCTGCTCACGTGTCGCAGCCTGCAAATCATGTGA
  CCTCTATGGCAAAAGCCATCATGCCGGTTTCTCTGTCTGAGGGGCGATCGACAGATAGTCCTGTCACTAT
  GAAGTCTTCATCTGAACCTGGTTATAAAATGAGCTCACCACAAGTTGTTTCTCCCAGTTCCTCAGACTCG
  CTCCCCCCAGGGACTCAGTGTCTAGTTGGAGCTATGGTCTCTGGAGGAGGCAGAAATGAGAGTTCTCCTG
  ATTCAAAGCGACTTTCCACTTCAGATATAAACAGCAGAGATTCCAAAAGGGTTAAACTCAGGGATGGAAT
  CCCAGGGGTACCTTCTTTAGCTGTGGTCCCTTCAGATATGTCTTCTACAATAAGCACAAATACACCCTCA
  CAGGGGATCTGCAACTCATCAAACCATGTTCAGAATGGAGTAACATTTCCTTGGCCTGATGCTAATGGAA
  AGGCACATTTCAATCTTACAGATCCAGAGAGAGCAAGTGAGTCTGCCCTGGCAATGACAGACATTTCAAG
  TCTAGCAAGTCAAAACAAGACCTTTGATCCCAAGAAAGAAAATCCCATCGTGTTACTTAGCGACTTTTAC
  TATGGACAGCATAAAGGAGATGGGCAGCCGGAACAGAAGACTCACACCACCTTTAAATGCCTCAGCTGCG
  TGAAAGTTCTAAAAAATATTAAGTTTATGAATCACATGAAGCATCATTTGGAATTTGAGAAGCAGAGGAA
  CGACAGCTGGGAAGACCACACCACCTGCCAGCACTGCCACCGGCAGTTTCCCACTCCCTTCCAGCTACAG
  TGTCACATTGATAGTGTACACATCGCCATGGGGCCCTCTGCTGTCTGTAAAATCTGTGAATTGTCATTTG
  AAACAGATCAGGTCCTCTTACAACACATGAAGGACCATCATAAGCCTGGCGAAATGCCTTATGTGTGCCA
  GGTTTGCCATTACAGATCGTCGGTCTTTGCTGATGTGGAAACACATTTTAGAACGTGCCATGAAAACACA
  AAGAATTTGCTTTGTCTGTTTTGTCTCAAACTTTTCAAAACTGCAATACCATACATGAATCATTGTTGGA
  GGCACAGCAGAAGGAGGGTCCTTCAGTGTTCCAAGTGCCGGCTACAGTTTTTGACGTTGAAGGAGGAAAT
  AGAGCACAAAACCAAGGACCATCAAACATTTAAAAAGCCGGAGCAACTGCAAGGGTTGCCTAGTGAAACA
  AAAGTTATTATTCAAACTTCAGTTCAGCCAGGATCAAGTGGTATGGCTTCCGTTATTGTTAGCAACACTG
  ACCCTCAGTCTTCTCCTGTAAAAACTAAAAAGAAGACGGCTATGAACACTAGAGATTCCAGACTCCCTTG
  CAGCAAGGATTCTAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208802 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGDIFLCKKVESPKKNLRESKQREEDDEDPDLIYVGVEHVHRDAEVLFVGMISNSKPVVSNILNRVTPGS
  NSRRKKGHFRQYPAHVSQPANHVTSMAKAIMPVSLSEGRSTDSPVTMKSSSEPGYKMSSPQVVSPSSSDS
  LPPGTQCLVGAMVSGGGRNESSPDSKRLSTSDINSRDSKRVKLRDGIPGVPSLAVVPSDMSSTISTNTPS
  QGICNSSNHVQNGVTFPWPDANGKAHFNLTDPERASESALAMTDISSLASQNKTFDPKKENPIVLLSDFY
  YGQHKGDGQPEQKTHTTFKCLSCVKVLKNIKFMNHMKHHLEFEKQRNDSWEDHTTCQHCHRQFPTPFQLQ
  CHIDSVHIAMGPSAVCKICELSFETDQVLLQHMKDHHKPGEMPYVCQVCHYRSSVFADVETHFRTCHENT
  KNLLCLFCLKLFKTAIPYMNHCWRHSRRRVLQCSKCRLQFLTLKEEIEHKTKDHQTFKKPEQLQGLPSET
  KVIIQTSVQPGSSGMASVIVSNTDPQSSPVKTKKKTAMNTRDSRLPCSKDSS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_080740
• ORF Size: 1626 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_080740.5
• RefSeq Size: 2175 bp
• RefSeq ORF: 1629 bp
• Locus ID: 129025
• UniProt ID: P59817
• Cytogenetics: 22q11.22
• Protein Families: Druggable Genome, Transcription Factors
• MW: 60.6 kDa
• Gene Summary: This gene encodes a zinc finger protein. The encoded protein contains 4 C2H2-type zinc fingers, which are commonly found in transcription factors. A variety of functions may be performed by this type of zinc finger protein, including the binding of DNA or RNA. [provided by RefSeq, Apr 2014]