RIAM (APBB1IP) (NM_019043) Human Tagged ORF Clone

CAT#: RC208712

APBB1IP (Myc-DDK-tagged)-Human amyloid beta (A4) precursor protein-binding, family B, member 1 interacting protein (APBB1IP)

Product Images

Circular map for RC208712

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY APBB1IP (Cat# RC208712, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-APBB1IP (Cat# TA809230)(1:2000). Positive lysates LY412750 (100ug) and LC412750 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY412750) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC208712 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified APBB1IP protein (Cat# TP308712). The protein was produced from HEK293T cells transfected with APBB1IP cDNA clone (Cat# RC208712) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RIAM
• Synonyms: INAG1; PREL1; RARP1; RIAM
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208712 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTGAGTCAAGTGAAGACATAGACCAAATGTTCAGCACTTTGCTGGGAGAGATGGATCTTCTGACTC
  AGAGTTTAGGAGTTGACACTCTCCCTCCTCCTGACCCTAATCCACCCAGAGCTGAATTTAACTACAGTGT
  GGGGTTTAAAGATTTAAATGAGTCCTTAAATGCACTGGAAGACCAAGATTTAGATGCTCTCATGGCAGAT
  CTGGTAGCAGACATAAGTGAGGCTGAGCAGAGGACAATCCAGGCACAGAAAGAGTCCTTGCAGAATCAAC
  ATCATTCAGCATCTCTACAAGCATCAATTTTCAGTGGTGCAGCCTCTCTTGGTTATGGAACAAATGTTGC
  TGCCACTGGTATCAGCCAATATGAGGATGACTTACCACCTCCACCAGCCGATCCTGTGTTAGACCTTCCA
  CTGCCACCACCACCTCCTGAACCTCTCTCTCAGGAAGAGGAAGAAGCCCAAGCCAAGGCTGATAAAATTA
  AGCTGGCGCTGGAAAAACTGAAGGAGGCCAAGGTTAAGAAGCTCGTCGTCAAGGTGCACATGAATGATAA
  CAGCACAAAGTCACTGATGGTGGATGAGCGGCAGCTGGCCCGAGATGTTCTGGACAACCTTTTCGAGAAA
  ACTCATTGTGACTGCAATGTAGACTGGTGTCTTTATGAAATCTACCCGGAACTACAAATTGAGAGGTTTT
  TTGAAGACCATGAAAATGTTGTTGAAGTCTTATCAGACTGGACAAGAGACACAGAAAATAAAATACTATT
  TTTGGAGAAAGAGGAGAAATATGCTGTATTTAAAAACCCCCAGAATTTCTACTTGGATAACAGAGGAAAA
  AAAGAAAGCAAGGAAACTAATGAGAAAATGAATGCTAAGAACAAGGAATCCTTACTTGAGGAAAGTTTCT
  GTGGAACATCTATCATTGTACCAGAACTGGAAGGAGCTCTTTATTTGAAAGAAGATGGAAAGAAATCCTG
  GAAAAGGCGCTATTTTCTTTTACGGGCTTCTGGAATTTATTATGTACCCAAAGGAAAGACTAAGACATCT
  CGAGATCTGGCGTGTTTTATACAGTTTGAAAATGTCAACATTTACTATGGGACTCAGCATAAAATGAAAT
  ATAAAGCGCCCACTGACTATTGCTTTGTTTTAAAGCACCCCCAAATTCAGAAGGAGTCCCAGTATATCAA
  GTATCTCTGCTGTGATGACACAAGAACCCTTAACCAGTGGGTCATGGGAATACGGATAGCCAAGTATGGG
  AAGACTCTCTATGATAACTACCAGCGGGCTGTGGCAAAGGCTGGACTTGCCTCTCGGTGGACAAACTTGG
  GGACAGTCAATGCAGCTGCACCAGCTCAGCCATCTACAGGACCTAAAACAGGCACCACCCAGCCCAATGG
  ACAGATTCCCCAGGCTACACATTCTGTCAGTGCTGTTCTCCAAGAGGCCCAGAGACATGCTGAAACATCG
  AAGGATAAGAAGCCAGCCCTCGGGAACCACCACGACCCGGCAGTGCCCCGGGCCCCGCACGCCCCCAAGT
  CCAGCCTGCCCCCGCCCCCTCCGGTGCGGAGGTCCTCCGACACCAGCGGCAGTCCCGCCACGCCCCTCAA
  GGCCAAGGGCACAGGCGGCGGGGGCTTGCCCGCCCCACCCGACGACTTCCTGCCGCCGCCGCCACCGCCG
  CCGCCCCTCGATGACCCTGAGCTCCCGCCGCCGCCCCCGGACTTCATGGAGCCGCCCCCAGACTTCGTGC
  CCCCGCCCCCGCCGTCGTACGCAGGGATCGCGGGCTCAGAGCTGCCCCCGCCGCCGCCGCCGCCGCCCGC
  GCCCGCGCCCGCCCCCGTCCCCGACTCCGCCAGGCCGCCCCCCGCGGTGGCCAAGAGGCCTCCTGTGCCC
  CCCAAGAGGCAAGAGAACCCAGGGCACCCCGGCGGAGCAGGAGGCGGGGAGCAAGATTTCATGTCAGACC
  TCATGAAAGCTTTGCAAAAGAAGAGAGGCAACGTGTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208712 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGESSEDIDQMFSTLLGEMDLLTQSLGVDTLPPPDPNPPRAEFNYSVGFKDLNESLNALEDQDLDALMAD
  LVADISEAEQRTIQAQKESLQNQHHSASLQASIFSGAASLGYGTNVAATGISQYEDDLPPPPADPVLDLP
  LPPPPPEPLSQEEEEAQAKADKIKLALEKLKEAKVKKLVVKVHMNDNSTKSLMVDERQLARDVLDNLFEK
  THCDCNVDWCLYEIYPELQIERFFEDHENVVEVLSDWTRDTENKILFLEKEEKYAVFKNPQNFYLDNRGK
  KESKETNEKMNAKNKESLLEESFCGTSIIVPELEGALYLKEDGKKSWKRRYFLLRASGIYYVPKGKTKTS
  RDLACFIQFENVNIYYGTQHKMKYKAPTDYCFVLKHPQIQKESQYIKYLCCDDTRTLNQWVMGIRIAKYG
  KTLYDNYQRAVAKAGLASRWTNLGTVNAAAPAQPSTGPKTGTTQPNGQIPQATHSVSAVLQEAQRHAETS
  KDKKPALGNHHDPAVPRAPHAPKSSLPPPPPVRRSSDTSGSPATPLKAKGTGGGGLPAPPDDFLPPPPPP
  PPLDDPELPPPPPDFMEPPPDFVPPPPPSYAGIAGSELPPPPPPPPAPAPAPVPDSARPPPAVAKRPPVP
  PKRQENPGHPGGAGGGEQDFMSDLMKALQKKRGNVS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019043
• ORF Size: 1998 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019043.4
• RefSeq Size: 2659 bp
• RefSeq ORF: 2001 bp
• Locus ID: 54518
• UniProt ID: Q7Z5R6
• Cytogenetics: 10p12.1
• Domains: RA, PH
• MW: 73.2 kDa
• Gene Summary: Appears to function in the signal transduction from Ras activation to actin cytoskeletal remodeling. Suppresses insulin-induced promoter activities through AP1 and SRE. Mediates Rap1-induced adhesion.[UniProtKB/Swiss-Prot Function]