EIF4EBP2 (NM_004096) Human Tagged ORF Clone
EIF4EBP2 (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 4E binding protein 2 (EIF4EBP2)
|Type||Human Tagged ORF Clone|
|E. coli Selection||Kanamycin (25 ug/mL)|
|Mammalian Cell Selection||Neomycin|
>RC208664 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)
>RC208664 protein sequence
Red=Cloning site Green=Tags(s)
Sequencher program is needed, download here.
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||360 bp|
|OTI Disclaimer||The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info|
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq Size||7531 bp|
|RefSeq ORF||363 bp|
|Protein Families||Transcription Factors|
|Gene Summary||This gene encodes a member of the eukaryotic translation initiation factor 4E binding protein family. The gene products of this family bind eIF4E and inhibit translation initiation. However, insulin and other growth factors can release this inhibition via a phosphorylation-dependent disruption of their binding to eIF4E. Regulation of protein production through these gene products have been implicated in cell proliferation, cell differentiation and viral infection. [provided by RefSeq, Oct 2008]|
|cDNA Clone Resources|
|RC208664L3||Lenti ORF clone of Human eukaryotic translation initiation factor 4E binding protein 2 (EIF4EBP2), Myc-DDK-tagged||
|RC208664L4||Lenti ORF clone of Human eukaryotic translation initiation factor 4E binding protein 2 (EIF4EBP2), mGFP tagged||
|RG208664||EIF4EBP2 (GFP-tagged) - Human eukaryotic translation initiation factor 4E binding protein 2 (EIF4EBP2)||
|SC117589||EIF4EBP2 (untagged)-Human eukaryotic translation initiation factor 4E binding protein 2 (EIF4EBP2)||