Oct-1 (POU2F1) (NM_002697) Human Tagged ORF Clone

CAT#: RC208599

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POU2F1 (Myc-DDK-tagged)-Human POU class 2 homeobox 1 (POU2F1), transcript variant 1



  "NM_002697" in other vectors (6)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 691.00


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In Stock

Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol POU2F1
Synonyms oct-1B; OCT1; OTF1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC208599 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAACAATCCGTCAGAAACCAGTAAACCATCTATGGAGAGTGGAGATGGCAACACAGGCACACAAACCA
ATGGTCTGGACTTTCAGAAGCAGCCTGTGCCTGTAGGAGGAGCAATCTCAACAGCCCAGGCGCAGGCTTT
CCTTGGACATCTCCATCAGGTCCAACTCGCTGGAACAAGTTTACAGGCTGCTGCTCAGTCTTTAAATGTA
CAGTCTAAATCTAATGAAGAATCGGGGGATTCGCAGCAGCCAAGCCAGCCTTCCCAGCAGCCTTCAGTGC
AGGCAGCCATTCCCCAGACCCAGCTTATGCTAGCTGGAGGACAGATAACTGGGCTTACTTTGACGCCTGC
CCAGCAACAGTTACTACTCCAGCAGGCACAGGCACAGGCACAGCTGCTGGCTGCTGCAGTGCAGCAGCAC
TCCGCCAGCCAGCAGCACAGTGCTGCTGGAGCCACCATCTCCGCCTCTGCTGCCACGCCCATGACGCAGA
TCCCCCTGTCTCAGCCCATACAGATCGCACAGGATCTTCAACAACTGCAACAGCTTCAACAGCAGAATCT
CAACCTGCAACAGTTTGTGTTGGTGCATCCAACCACCAATTTGCAGCCAGCGCAGTTTATCATCTCACAG
ACGCCCCAGGGCCAGCAGGGTCTCCTGCAAGCGCAAAATCTTCTAACGCAACTACCTCAGCAAAGCCAAG
CCAACCTCCTACAGTCGCAGCCAAGCATCACCCTCACCTCCCAGCCAGCAACCCCAACACGCACAATAGC
AGCAACCCCAATTCAGACACTTCCACAGAGCCAGTCAACACCAAAGCGAATTGATACTCCCAGCTTGGAG
GAGCCCAGTGACCTTGAGGAGCTTGAGCAGTTTGCCAAGACCTTCAAACAAAGACGAATCAAACTTGGAT
TCACTCAGGGTGATGTTGGGCTCGCTATGGGGAAACTATATGGAAATGACTTCAGCCAAACTACCATCTC
TCGATTTGAAGCCTTGAACCTCAGCTTTAAGAACATGTGCAAGTTGAAGCCACTTTTAGAGAAGTGGCTA
AATGATGCAGAGAACCTCTCATCTGATTCGTCCCTCTCCAGCCCAAGTGCCCTGAATTCTCCAGGAATTG
AGGGCTTGAGCCGTAGGAGGAAGAAACGCACCAGCATAGAGACCAACATCCGTGTGGCCTTAGAGAAGAG
TTTCTTGGAGAATCAAAAGCCTACCTCGGAAGAGATCACTATGATTGCTGATCAGCTCAATATGGAAAAA
GAGGTGATTCGTGTTTGGTTCTGTAACCGCCGCCAGAAAGAAAAAAGAATCAACCCACCAAGCAGTGGTG
GGACCAGCAGCTCACCTATTAAAGCAATTTTCCCCAGCCCAACTTCACTGGTGGCGACCACACCAAGCCT
TGTGACTAGCAGTGCAGCAACTACCCTCACAGTCAGCCCTGTCCTCCCTCTGACCAGTGCTGCTGTGACG
AATCTTTCAGTTACAGGCACTTCAGACACCACCTCCAACAACACAGCAACCGTGATTTCCACAGCGCCTC
CAGCTTCCTCAGCAGTCACGTCCCCCTCTCTGAGTCCCTCCCCTTCTGCCTCAGCCTCCACCTCCGAGGC
ATCCAGTGCCAGTGAGACCAGCACAACACAGACCACCTCCACTCCTTTGTCCTCCCCTCTTGGGACCAGC
CAGGTGATGGTGACAGCATCAGGTTTGCAAACAGCAGCAGCTGCTGCCCTTCAAGGAGCTGCACAGTTGC
CAGCAAATGCCAGTCTTGCTGCCATGGCAGCTGCTGCAGGACTAAACCCAAGCCTGATGGCACCCTCACA
GTTTGCGGCTGGAGGTGCCTTACTCAGTCTGAATCCAGGGACCCTGAGCGGTGCTCTCAGCCCAGCTCTA
ATGAGCAACAGTACACTGGCAACTATTCAAGCTCTTGCTTCTGGTGGCTCTCTTCCAATAACATCACTTG
ATGCAACTGGGAACCTGGTATTTGCCAATGCGGGAGGAGCCCCCAACATCGTGACTGCCCCTCTGTTCCT
GAACCCTCAGAACCTCTCTCTGCTCACCAGCAACCCTGTTAGCTTGGTCTCTGCCGCCGCAGCATCTGCA
GGGAACTCTGCACCTGTAGCCAGCCTTCACGCCACCTCCACCTCTGCTGAGTCCATCCAGAACTCTCTCT
TCACAGTGGCCTCTGCCAGCGGGGCTGCGTCCACCACCACCACCGCCTCCAAGGCACAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC208599 protein sequence
Red=Cloning site Green=Tags(s)

MNNPSETSKPSMESGDGNTGTQTNGLDFQKQPVPVGGAISTAQAQAFLGHLHQVQLAGTSLQAAAQSLNV
QSKSNEESGDSQQPSQPSQQPSVQAAIPQTQLMLAGGQITGLTLTPAQQQLLLQQAQAQAQLLAAAVQQH
SASQQHSAAGATISASAATPMTQIPLSQPIQIAQDLQQLQQLQQQNLNLQQFVLVHPTTNLQPAQFIISQ
TPQGQQGLLQAQNLLTQLPQQSQANLLQSQPSITLTSQPATPTRTIAATPIQTLPQSQSTPKRIDTPSLE
EPSDLEELEQFAKTFKQRRIKLGFTQGDVGLAMGKLYGNDFSQTTISRFEALNLSFKNMCKLKPLLEKWL
NDAENLSSDSSLSSPSALNSPGIEGLSRRRKKRTSIETNIRVALEKSFLENQKPTSEEITMIADQLNMEK
EVIRVWFCNRRQKEKRINPPSSGGTSSSPIKAIFPSPTSLVATTPSLVTSSAATTLTVSPVLPLTSAAVT
NLSVTGTSDTTSNNTATVISTAPPASSAVTSPSLSPSPSASASTSEASSASETSTTQTTSTPLSSPLGTS
QVMVTASGLQTAAAAALQGAAQLPANASLAAMAAAAGLNPSLMAPSQFAAGGALLSLNPGTLSGALSPAL
MSNSTLATIQALASGGSLPITSLDATGNLVFANAGGAPNIVTAPLFLNPQNLSLLTSNPVSLVSAAAASA
GNSAPVASLHATSTSAESIQNSLFTVASASGAASTTTTASKAQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_002697
ORF Size 2229 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_002697.2, NP_002688.2
RefSeq Size 13905 bp
RefSeq ORF 2301 bp
Locus ID 5451
UniProt ID P14859
Cytogenetics 1q24.2
Domains POU, homeobox
Protein Families Druggable Genome, Transcription Factors
MW 76.5 kDa
Gene Summary The OCT1 transcription factor was among the first identified members of the POU transcription factor family (summarized by Sturm et al., 1993 [PubMed 8314572]). Members of this family contain the POU domain, a 160-amino acid region necessary for DNA binding to the octameric sequence ATGCAAAT.[supplied by OMIM, Jul 2010]

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