Oct-1 (POU2F1) (NM_002697) Human Tagged ORF Clone

CAT#: RC208599

POU2F1 (Myc-DDK-tagged)-Human POU class 2 homeobox 1 (POU2F1), transcript variant 1

Product Images

Circular map for RC208599

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  Western blot validation of overexpression lysate (Cat# LY419163) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC208599 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified POU2F1 protein (Cat# TP308599). The protein was produced from HEK293T cells transfected with POU2F1 cDNA clone (Cat# RC208599) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Oct-1
• Synonyms: oct-1B; OCT1; OTF1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208599 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACAATCCGTCAGAAACCAGTAAACCATCTATGGAGAGTGGAGATGGCAACACAGGCACACAAACCA
  ATGGTCTGGACTTTCAGAAGCAGCCTGTGCCTGTAGGAGGAGCAATCTCAACAGCCCAGGCGCAGGCTTT
  CCTTGGACATCTCCATCAGGTCCAACTCGCTGGAACAAGTTTACAGGCTGCTGCTCAGTCTTTAAATGTA
  CAGTCTAAATCTAATGAAGAATCGGGGGATTCGCAGCAGCCAAGCCAGCCTTCCCAGCAGCCTTCAGTGC
  AGGCAGCCATTCCCCAGACCCAGCTTATGCTAGCTGGAGGACAGATAACTGGGCTTACTTTGACGCCTGC
  CCAGCAACAGTTACTACTCCAGCAGGCACAGGCACAGGCACAGCTGCTGGCTGCTGCAGTGCAGCAGCAC
  TCCGCCAGCCAGCAGCACAGTGCTGCTGGAGCCACCATCTCCGCCTCTGCTGCCACGCCCATGACGCAGA
  TCCCCCTGTCTCAGCCCATACAGATCGCACAGGATCTTCAACAACTGCAACAGCTTCAACAGCAGAATCT
  CAACCTGCAACAGTTTGTGTTGGTGCATCCAACCACCAATTTGCAGCCAGCGCAGTTTATCATCTCACAG
  ACGCCCCAGGGCCAGCAGGGTCTCCTGCAAGCGCAAAATCTTCTAACGCAACTACCTCAGCAAAGCCAAG
  CCAACCTCCTACAGTCGCAGCCAAGCATCACCCTCACCTCCCAGCCAGCAACCCCAACACGCACAATAGC
  AGCAACCCCAATTCAGACACTTCCACAGAGCCAGTCAACACCAAAGCGAATTGATACTCCCAGCTTGGAG
  GAGCCCAGTGACCTTGAGGAGCTTGAGCAGTTTGCCAAGACCTTCAAACAAAGACGAATCAAACTTGGAT
  TCACTCAGGGTGATGTTGGGCTCGCTATGGGGAAACTATATGGAAATGACTTCAGCCAAACTACCATCTC
  TCGATTTGAAGCCTTGAACCTCAGCTTTAAGAACATGTGCAAGTTGAAGCCACTTTTAGAGAAGTGGCTA
  AATGATGCAGAGAACCTCTCATCTGATTCGTCCCTCTCCAGCCCAAGTGCCCTGAATTCTCCAGGAATTG
  AGGGCTTGAGCCGTAGGAGGAAGAAACGCACCAGCATAGAGACCAACATCCGTGTGGCCTTAGAGAAGAG
  TTTCTTGGAGAATCAAAAGCCTACCTCGGAAGAGATCACTATGATTGCTGATCAGCTCAATATGGAAAAA
  GAGGTGATTCGTGTTTGGTTCTGTAACCGCCGCCAGAAAGAAAAAAGAATCAACCCACCAAGCAGTGGTG
  GGACCAGCAGCTCACCTATTAAAGCAATTTTCCCCAGCCCAACTTCACTGGTGGCGACCACACCAAGCCT
  TGTGACTAGCAGTGCAGCAACTACCCTCACAGTCAGCCCTGTCCTCCCTCTGACCAGTGCTGCTGTGACG
  AATCTTTCAGTTACAGGCACTTCAGACACCACCTCCAACAACACAGCAACCGTGATTTCCACAGCGCCTC
  CAGCTTCCTCAGCAGTCACGTCCCCCTCTCTGAGTCCCTCCCCTTCTGCCTCAGCCTCCACCTCCGAGGC
  ATCCAGTGCCAGTGAGACCAGCACAACACAGACCACCTCCACTCCTTTGTCCTCCCCTCTTGGGACCAGC
  CAGGTGATGGTGACAGCATCAGGTTTGCAAACAGCAGCAGCTGCTGCCCTTCAAGGAGCTGCACAGTTGC
  CAGCAAATGCCAGTCTTGCTGCCATGGCAGCTGCTGCAGGACTAAACCCAAGCCTGATGGCACCCTCACA
  GTTTGCGGCTGGAGGTGCCTTACTCAGTCTGAATCCAGGGACCCTGAGCGGTGCTCTCAGCCCAGCTCTA
  ATGAGCAACAGTACACTGGCAACTATTCAAGCTCTTGCTTCTGGTGGCTCTCTTCCAATAACATCACTTG
  ATGCAACTGGGAACCTGGTATTTGCCAATGCGGGAGGAGCCCCCAACATCGTGACTGCCCCTCTGTTCCT
  GAACCCTCAGAACCTCTCTCTGCTCACCAGCAACCCTGTTAGCTTGGTCTCTGCCGCCGCAGCATCTGCA
  GGGAACTCTGCACCTGTAGCCAGCCTTCACGCCACCTCCACCTCTGCTGAGTCCATCCAGAACTCTCTCT
  TCACAGTGGCCTCTGCCAGCGGGGCTGCGTCCACCACCACCACCGCCTCCAAGGCACAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208599 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MNNPSETSKPSMESGDGNTGTQTNGLDFQKQPVPVGGAISTAQAQAFLGHLHQVQLAGTSLQAAAQSLNV
  QSKSNEESGDSQQPSQPSQQPSVQAAIPQTQLMLAGGQITGLTLTPAQQQLLLQQAQAQAQLLAAAVQQH
  SASQQHSAAGATISASAATPMTQIPLSQPIQIAQDLQQLQQLQQQNLNLQQFVLVHPTTNLQPAQFIISQ
  TPQGQQGLLQAQNLLTQLPQQSQANLLQSQPSITLTSQPATPTRTIAATPIQTLPQSQSTPKRIDTPSLE
  EPSDLEELEQFAKTFKQRRIKLGFTQGDVGLAMGKLYGNDFSQTTISRFEALNLSFKNMCKLKPLLEKWL
  NDAENLSSDSSLSSPSALNSPGIEGLSRRRKKRTSIETNIRVALEKSFLENQKPTSEEITMIADQLNMEK
  EVIRVWFCNRRQKEKRINPPSSGGTSSSPIKAIFPSPTSLVATTPSLVTSSAATTLTVSPVLPLTSAAVT
  NLSVTGTSDTTSNNTATVISTAPPASSAVTSPSLSPSPSASASTSEASSASETSTTQTTSTPLSSPLGTS
  QVMVTASGLQTAAAAALQGAAQLPANASLAAMAAAAGLNPSLMAPSQFAAGGALLSLNPGTLSGALSPAL
  MSNSTLATIQALASGGSLPITSLDATGNLVFANAGGAPNIVTAPLFLNPQNLSLLTSNPVSLVSAAAASA
  GNSAPVASLHATSTSAESIQNSLFTVASASGAASTTTTASKAQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002697
• ORF Size: 2229 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002697.2, NP_002688.2
• RefSeq Size: 13905 bp
• RefSeq ORF: 2301 bp
• Locus ID: 5451
• UniProt ID: P14859
• Cytogenetics: 1q24.2
• Domains: POU, homeobox
• Protein Families: Druggable Genome, Transcription Factors
• MW: 76.5 kDa
• Gene Summary: The OCT1 transcription factor was among the first identified members of the POU transcription factor family (summarized by Sturm et al., 1993 [PubMed 8314572]). Members of this family contain the POU domain, a 160-amino acid region necessary for DNA binding to the octameric sequence ATGCAAAT.[supplied by OMIM, Jul 2010]