MLYCD (NM_012213) Human Tagged ORF Clone

CAT#: RC208587

MLYCD (Myc-DDK-tagged)-Human malonyl-CoA decarboxylase (MLYCD), nuclear gene encoding mitochondrial protein

Product Images

Circular map for RC208587

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  Western blot validation of overexpression lysate (Cat# LY415904) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC208587 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified MLYCD protein (Cat# TP308587). The protein was produced from HEK293T cells transfected with MLYCD cDNA clone (Cat# RC208587) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MLYCD
• Synonyms: MCD
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208587 representing NM_012213
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGAGGCTTCGGGCCAGGCTTGACGGCCAGGCGTCTCCTCCCGCTGCGGTTGCCCCCGCGGCCGCCCG
  GGCCCCGGCTGGCGAGCGGGCAGGCGGCCGGCGCCCTGGAGCGGGCCATGGACGAGCTGCTGCGCCGCGC
  GGTGCCGCCGACGCCGGCCTACGAGCTGCGCGAGAAGACACCGGCGCCCGCCGAGGGTCAGTGCGCGGAC
  TTCGTGAGCTTCTACGGTGGGCTGGCCGAGACGGCCCAGCGGGCCGAACTGCTGGGCCGCCTGGCGCGGG
  GCTTCGGCGTGGACCACGGCCAGGTGGCGGAGCAGAGCGCCGGCGTGCTCCATCTGCGCCAGCAGCAGCG
  GGAGGCGGCGGTGCTGCTGCAGGCCGAGGACCGGCTGCGCTACGCGCTGGTGCCGCGCTATCGCGGCCTC
  TTCCACCACATCAGCAAGCTGGACGGCGGCGTGCGCTTCCTGGTGCAGCTGCGGGCCGACCTGCTGGAGG
  CGCAGGCCCTCAAGCTGGTGGAGGGGCCGGACGTCCGGGAAATGAATGGGGTGCTGAAAGGAATGCTCTC
  AGAATGGTTTTCCTCCGGGTTCCTGAACCTAGAACGGGTTACCTGGCATTCACCGTGTGAAGTGCTTCAG
  AAAATCAGTGAGGCTGAGGCTGTGCATCCTGTAAAAAACTGGATGGACATGAAGCGCCGCGTTGGGCCCT
  ACAGAAGGTGTTACTTCTTTTCTCACTGTTCGACCCCTGGGGAGCCCCTGGTCGTTTTGCACGTGGCACT
  GACTGGTGACATCTCCAGCAACATCCAGGCAATCGTGAAGGAACATCCTCCATCAGAAACAGAAGAGAAG
  AACAAAATCACTGCTGCGATCTTTTATTCCATCAGCTTGACCCAGCAGGGACTCCAAGGGGTGGAGCTGG
  GAACATTCCTCATAAAGCGAGTCGTCAAGGAGTTGCAGAGAGAGTTTCCTCACCTTGGGGTGTTTTCAAG
  TCTGTCACCTATACCTGGTTTCACCAAATGGCTTCTGGGGCTTCTGAACTCGCAAACGAAGGAGCATGGG
  AGGAATGAACTCTTTACAGATTCGGAATGTAAGGAAATCTCGGAGATCACAGGTGGCCCCATTAACGAGA
  CCCTCAAGCTCCTCCTCAGCAGCAGCGAGTGGGTGCAGTCGGAGAAGCTGGTGCGGGCGCTGCAGACTCC
  GCTGATGAGGCTGTGCGCCTGGTACCTGTATGGAGAGAAGCACCGCGGCTACGCGCTGAACCCCGTGGCC
  AACTTCCACCTGCAGAACGGGGCGGTGCTGTGGCGCATCAACTGGATGGCGGATGTGAGCCTCAGAGGCA
  TCACCGGCTCCTGCGGCCTGATGGCCAACTACCGCTACTTCCTGGAGGAGACGGGCCCCAACAGCACCTC
  CTACCTCGGCTCCAAGATCATCAAAGCCTCTGAGCAGGTCCTCAGCCTAGTGGCCCAGTTTCAAAAGAAC
  AGCAAGCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208587 representing NM_012213
  Red=Cloning site Green=Tags(s)
  
  MRGFGPGLTARRLLPLRLPPRPPGPRLASGQAAGALERAMDELLRRAVPPTPAYELREKTPAPAEGQCAD
  FVSFYGGLAETAQRAELLGRLARGFGVDHGQVAEQSAGVLHLRQQQREAAVLLQAEDRLRYALVPRYRGL
  FHHISKLDGGVRFLVQLRADLLEAQALKLVEGPDVREMNGVLKGMLSEWFSSGFLNLERVTWHSPCEVLQ
  KISEAEAVHPVKNWMDMKRRVGPYRRCYFFSHCSTPGEPLVVLHVALTGDISSNIQAIVKEHPPSETEEK
  NKITAAIFYSISLTQQGLQGVELGTFLIKRVVKELQREFPHLGVFSSLSPIPGFTKWLLGLLNSQTKEHG
  RNELFTDSECKEISEITGGPINETLKLLLSSSEWVQSEKLVRALQTPLMRLCAWYLYGEKHRGYALNPVA
  NFHLQNGAVLWRINWMADVSLRGITGSCGLMANYRYFLEETGPNSTSYLGSKIIKASEQVLSLVAQFQKN
  SKL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012213
• ORF Size: 1479 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012213.3
• RefSeq Size: 2211 bp
• RefSeq ORF: 1482 bp
• Locus ID: 23417
• UniProt ID: O95822
• Cytogenetics: 16q23.3
• Domains: MCD
• Protein Families: Druggable Genome
• Protein Pathways: beta-Alanine metabolism, Metabolic pathways, Propanoate metabolism
• MW: 54.8 kDa
• Gene Summary: The product of this gene catalyzes the breakdown of malonyl-CoA to acetyl-CoA and carbon dioxide. Malonyl-CoA is an intermediate in fatty acid biosynthesis, and also inhibits the transport of fatty acyl CoAs into mitochondria. Consequently, the encoded protein acts to increase the rate of fatty acid oxidation. It is found in mitochondria, peroxisomes, and the cytoplasm. Mutations in this gene result in malonyl-CoA decarboyxlase deficiency. [provided by RefSeq, Jul 2008]